Biosecurity Molecular Screening Using eDNA Technology

The primary objective of this project is to trial portable sequencing devices to determine the applicability of eDNA for operational use across many different import pathways. The performance of the Oxford Nanopore MinION device will be compared to the standard Illumina MiSeq platform in the ability to identify the imported goods to species and priority pests and diseases of biosecurity concern. The project will develop a pathway DNA reference database (goods, pests and diseases) and expand the eDNA library to include priority biosecurity pests and diseases. It will also lay foundations for the development of a national reference laboratory capability in eDNA research and testing for biosecurity.