原位杂交技术在检测 HBV 核酸和 cccDNA 中的应用

Tong Xu; Xiaonan Zhang; Zhenghong Yuan

doi:10.3969/j.issn.1001-5256.2019.06.005

原位杂交技术在检测 HBV 核酸和 cccDNA 中的应用

Translated title of the contribution: Application of in situ hybridization in the detection of hepatitis B virus nucleic acids and cccDNA

Tong Xu, Xiaonan Zhang, Zhenghong Yuan

Research output: Contribution to journal › Review article › peer-review

Abstract

In situ hybridization (ISH)is a new technique which combines molecular biology, histochemistry, and cytology. It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research. ISH is of great significance for the detection of hepatitis B virus (HBV)nucleic acids (RNA and replicative intermediate DNA)and covalently closed circular DNA. This article reviews the development of ISH and its application in HBV research.

Translated title of the contribution	Application of in situ hybridization in the detection of hepatitis B virus nucleic acids and cccDNA
Original language	Chinese (Simplified)
Pages (from-to)	1197-1200
Number of pages	4
Journal	Journal of Clinical Hepatology
Volume	35
Issue number	6
DOIs	https://doi.org/10.3969/j.issn.1001-5256.2019.06.005
Publication status	Published - Jun 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3969/j.issn.1001-5256.2019.06.005

Cite this

@article{c2f4d10e75524b66a87d961bc708191c,

title = "原位杂交技术在检测 HBV 核酸和 cccDNA 中的应用",

abstract = "In situ hybridization (ISH)is a new technique which combines molecular biology, histochemistry, and cytology. It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research. ISH is of great significance for the detection of hepatitis B virus (HBV)nucleic acids (RNA and replicative intermediate DNA)and covalently closed circular DNA. This article reviews the development of ISH and its application in HBV research.",

keywords = "cccDNA, hepatitis B virus, in situ hybridization",

author = "Tong Xu and Xiaonan Zhang and Zhenghong Yuan",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s).",

year = "2019",

month = jun,

doi = "10.3969/j.issn.1001-5256.2019.06.005",

language = "Chinese (Simplified)",

volume = "35",

pages = "1197--1200",

journal = "Journal of Clinical Hepatology",

issn = "1001-5256",

number = "6",

}

TY - JOUR

T1 - 原位杂交技术在检测 HBV 核酸和 cccDNA 中的应用

AU - Xu, Tong

AU - Zhang, Xiaonan

AU - Yuan, Zhenghong

PY - 2019/6

Y1 - 2019/6

N2 - In situ hybridization (ISH)is a new technique which combines molecular biology, histochemistry, and cytology. It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research. ISH is of great significance for the detection of hepatitis B virus (HBV)nucleic acids (RNA and replicative intermediate DNA)and covalently closed circular DNA. This article reviews the development of ISH and its application in HBV research.

AB - In situ hybridization (ISH)is a new technique which combines molecular biology, histochemistry, and cytology. It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research. ISH is of great significance for the detection of hepatitis B virus (HBV)nucleic acids (RNA and replicative intermediate DNA)and covalently closed circular DNA. This article reviews the development of ISH and its application in HBV research.

KW - cccDNA

KW - hepatitis B virus

KW - in situ hybridization

UR - http://www.scopus.com/inward/record.url?scp=85149815355&partnerID=8YFLogxK

U2 - 10.3969/j.issn.1001-5256.2019.06.005

DO - 10.3969/j.issn.1001-5256.2019.06.005

M3 - Review article

AN - SCOPUS:85149815355

SN - 1001-5256

VL - 35

SP - 1197

EP - 1200

JO - Journal of Clinical Hepatology

JF - Journal of Clinical Hepatology

IS - 6

ER -

原位杂交技术在检测 HBV 核酸和 cccDNA 中的应用

Abstract

UN SDGs

Access to Document

Other files and links

Cite this