@inbook{320d0cf5d32e4f51b88f07a562012c40,
title = "A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue",
abstract = "Hepatitis B virus (HBV) developed highly intricates mechanisms exploiting host resources for its multiplication within a constrained genetic coding capacity. With the aid of a series of classical analytical methods such as ultrafiltration, and Southern and Northern blots, a general framework of HBV life cycle has been established. However, this picture still lacks many key histological contexts which involves pathophysiological changes of hepatocytes, non-parenchymal cells, infiltrated leukocytes, and associated extracellular matrix. Here, we describe a CISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in liver tissue of chronic hepatitis B patients. By coupling it with immunohistochemistry and other histological stains, much richer information regarding the HBV-induced pathological changes can be harvested.",
keywords = "cccDNA, CISH, HBV, Immunohistochemistry",
author = "Xiaonan Zhang and Wei Lu and Yanling Feng and Zhanqing Zhang and Zhenghong Yuan",
note = "Publisher Copyright: {\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. Funding Information: This work is supported by the Chinese National Natural Science Foundation (81873962, 32070152) and by Australian Centre for HIV and Hepatitis Virology Research (ACH2 ). Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-4027-2_12",
language = "English",
isbn = "9781071640265",
volume = "2837",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "137--148",
editor = "Haitao Guo and Andrea Cuconati",
booktitle = "Hepatitis B Virus",
address = "Netherlands",
edition = "2",
}
