@inbook{8d78f209221d4aa6a89350729a7f2629,
title = "A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture",
abstract = "Hepatitis B virus (HBV) is undoubtedly a master in exploiting host resources while evading host defense for its multiplication within a constrained genetic coding capacity. To further unravel these cunning strategies, a clear picture of virus-host interaction with key subcellular and molecular contexts is needed. Here, we describe a FISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in cell culture models (e.g., HepAD38, HepG2-NTCP). It can be coupled with immunofluorescence staining of viral or host proteins or other fluorescent tagging systems which could illuminate numerous aspects of virus-host interactions.",
keywords = "cccDNA, FISH, HBV",
author = "Xiaonan Zhang and Lei Yue and Chang Li and Zhenghong Yuan",
note = "Funding Information: This work is supported by the Chinese National Natural Science Foundation (81873962, 32070152) and by Australian Centre for HIV and Hepatitis Virology Research (ACH2 ). Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-4027-2_11",
language = "English",
isbn = "9781071640265",
volume = "2837",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "125--135",
editor = "Guo, {Haitao } and Cuconati, {Andrea }",
booktitle = "Hepatitis B Virus",
address = "Netherlands",
edition = "2",
}
