A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture

Xiaonan Zhang; Lei Yue; Chang Li; Zhenghong Yuan

doi:10.1007/978-1-0716-4027-2_11

A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture

Xiaonan Zhang
, Lei Yue
, Chang Li
, Zhenghong Yuan

Research output: A Conference proceeding or a Chapter in Book › Chapter › peer-review

Abstract

Hepatitis B virus (HBV) is undoubtedly a master in exploiting host resources while evading host defense for its multiplication within a constrained genetic coding capacity. To further unravel these cunning strategies, a clear picture of virus-host interaction with key subcellular and molecular contexts is needed. Here, we describe a FISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in cell culture models (e.g., HepAD38, HepG2-NTCP). It can be coupled with immunofluorescence staining of viral or host proteins or other fluorescent tagging systems which could illuminate numerous aspects of virus-host interactions.

Original language	English
Title of host publication	Hepatitis B Virus
Subtitle of host publication	Methods and Protocols
Editors	Haitao Guo, Andrea Cuconati
Publisher	Springer
Chapter	11
Pages	125-135
Number of pages	11
Volume	2837
Edition	2
ISBN (Electronic)	9781071640296
ISBN (Print)	9781071640265
DOIs	https://doi.org/10.1007/978-1-0716-4027-2_11
Publication status	Published - 2024

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1007/978-1-0716-4027-2_11

Cite this

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abstract = "Hepatitis B virus (HBV) is undoubtedly a master in exploiting host resources while evading host defense for its multiplication within a constrained genetic coding capacity. To further unravel these cunning strategies, a clear picture of virus-host interaction with key subcellular and molecular contexts is needed. Here, we describe a FISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in cell culture models (e.g., HepAD38, HepG2-NTCP). It can be coupled with immunofluorescence staining of viral or host proteins or other fluorescent tagging systems which could illuminate numerous aspects of virus-host interactions.",

keywords = "cccDNA, FISH, HBV",

author = "Xiaonan Zhang and Lei Yue and Chang Li and Zhenghong Yuan",

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A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture. / Zhang, Xiaonan; Yue, Lei; Li, Chang et al.
Hepatitis B Virus: Methods and Protocols. ed. / Haitao Guo; Andrea Cuconati. Vol. 2837 2. ed. Springer, 2024. p. 125-135 (Methods in molecular biology (Clifton, N.J.)).

Research output: A Conference proceeding or a Chapter in Book › Chapter › peer-review

TY - CHAP

T1 - A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture

AU - Zhang, Xiaonan

AU - Yue, Lei

AU - Li, Chang

AU - Yuan, Zhenghong

N1 - Funding Information: This work is supported by the Chinese National Natural Science Foundation (81873962, 32070152) and by Australian Centre for HIV and Hepatitis Virology Research (ACH2 ). Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

PY - 2024

Y1 - 2024

N2 - Hepatitis B virus (HBV) is undoubtedly a master in exploiting host resources while evading host defense for its multiplication within a constrained genetic coding capacity. To further unravel these cunning strategies, a clear picture of virus-host interaction with key subcellular and molecular contexts is needed. Here, we describe a FISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in cell culture models (e.g., HepAD38, HepG2-NTCP). It can be coupled with immunofluorescence staining of viral or host proteins or other fluorescent tagging systems which could illuminate numerous aspects of virus-host interactions.

AB - Hepatitis B virus (HBV) is undoubtedly a master in exploiting host resources while evading host defense for its multiplication within a constrained genetic coding capacity. To further unravel these cunning strategies, a clear picture of virus-host interaction with key subcellular and molecular contexts is needed. Here, we describe a FISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in cell culture models (e.g., HepAD38, HepG2-NTCP). It can be coupled with immunofluorescence staining of viral or host proteins or other fluorescent tagging systems which could illuminate numerous aspects of virus-host interactions.

KW - cccDNA

KW - FISH

KW - HBV

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DO - 10.1007/978-1-0716-4027-2_11

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AN - SCOPUS:85199340481

SN - 9781071640265

VL - 2837

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 125

EP - 135

BT - Hepatitis B Virus

A2 - Guo, Haitao

A2 - Cuconati, Andrea

PB - Springer

ER -

A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture

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