A framework for developing and validating taxon-specific primers for specimen identification from environmental DNA

Keyphrases

• Environmental DNA (eDNA) 100%
• Specimen Identification 100%
• Taxon-specific Primers 100%
• Ecological Study 40%
• DNA Testing 40%
• DNA Samples 20%
• False-negative Results 20%
• Laboratory Analysis 20%
• Wildlife 20%
• DNA Analysis 20%
• DNA Sequencing 20%
• Rigorous Evaluation 20%
• Primer Design 20%
• Error Sources 20%
• PCR Primers 20%
• Five Steps 20%
• Bandicoot 20%
• Database Construction 20%
• Risk of Error 20%
• In Silico Evaluation 20%
• Risk of Bias 20%
• Taxon-specific 20%
• Incremental Risk 20%
• Large-scale Implementation 20%
• Effective Application 20%
• Laboratory Evaluation 20%
• Mitochondrial ND2 Gene 20%
• Primer Evaluation 20%
• Primer Development 20%
• Primer Specificity 20%
• Laboratory Resources 20%

Biochemistry, Genetics and Molecular Biology

• Taxon 100%
• Ecological Study 50%
• DNA Sequence 25%
• DNA Analysis 25%
• Reference Database 25%
• Bandicoot 25%

Agricultural and Biological Sciences

• Ecological Study 100%
• Australians 50%