A micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in Atlantic Canada

B Hargrave, Lisa Doucette, Kats Haya, Faron Friars, Stephen Armstrong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A micro-dilution technique with changes in optical density (OD) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 mg OTC (oxytetracycline) ml -1 was applied to determine growth inhibition (GI) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. Growth of control cultures (Aeromonas salmonicida) was inhibited at all OTC concentrations. Some feed pellet samples and under-cage sediments from salmon aquaculture sites in the Bay of Fundy showed GI up to P160 mg OTC ml -1. Lower values (40 to 80 mg OTC ml -1) occurred in subsurface (5-18 cm) sediments, >100 m from salmon pens and adjacent to sewage outfalls in Halifax Harbour. GI values
Original languageEnglish
Pages (from-to)1439-1445
Number of pages7
JournalMarine Pollution Bulletin
Volume56
Issue number56 (2008) 1439â¿¿1445
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Aquaculture
oxytetracycline
marine sediments
Ports and harbors
Dilution
marine sediment
salmon
aquaculture
mussels
Bacteria
Sediments
harbor
dilution
Canada
growth retardation
bacterium
pelleted feeds
bacteria
sediments
Aeromonas salmonicida

Cite this

Hargrave, B ; Doucette, Lisa ; Haya, Kats ; Friars, Faron ; Armstrong, Stephen. / A micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in Atlantic Canada. In: Marine Pollution Bulletin. 2008 ; Vol. 56, No. 56 (2008) 1439â¿¿1445. pp. 1439-1445.
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abstract = "A micro-dilution technique with changes in optical density (OD) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 mg OTC (oxytetracycline) ml -1 was applied to determine growth inhibition (GI) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. Growth of control cultures (Aeromonas salmonicida) was inhibited at all OTC concentrations. Some feed pellet samples and under-cage sediments from salmon aquaculture sites in the Bay of Fundy showed GI up to P160 mg OTC ml -1. Lower values (40 to 80 mg OTC ml -1) occurred in subsurface (5-18 cm) sediments, >100 m from salmon pens and adjacent to sewage outfalls in Halifax Harbour. GI values",
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author = "B Hargrave and Lisa Doucette and Kats Haya and Faron Friars and Stephen Armstrong",
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A micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in Atlantic Canada. / Hargrave, B; Doucette, Lisa; Haya, Kats; Friars, Faron; Armstrong, Stephen.

In: Marine Pollution Bulletin, Vol. 56, No. 56 (2008) 1439â¿¿1445, 2008, p. 1439-1445.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in Atlantic Canada

AU - Hargrave, B

AU - Doucette, Lisa

AU - Haya, Kats

AU - Friars, Faron

AU - Armstrong, Stephen

PY - 2008

Y1 - 2008

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AB - A micro-dilution technique with changes in optical density (OD) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 mg OTC (oxytetracycline) ml -1 was applied to determine growth inhibition (GI) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. Growth of control cultures (Aeromonas salmonicida) was inhibited at all OTC concentrations. Some feed pellet samples and under-cage sediments from salmon aquaculture sites in the Bay of Fundy showed GI up to P160 mg OTC ml -1. Lower values (40 to 80 mg OTC ml -1) occurred in subsurface (5-18 cm) sediments, >100 m from salmon pens and adjacent to sewage outfalls in Halifax Harbour. GI values

KW - Antibiotics

KW - Terramycin AquaTM

KW - Microplate reader

KW - Aeromonas salmonicid

U2 - 10.1016/j.marpolbul.2008.04.044

DO - 10.1016/j.marpolbul.2008.04.044

M3 - Article

VL - 56

SP - 1439

EP - 1445

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

SN - 0025-326X

IS - 56 (2008) 1439â¿¿1445

ER -