A micro-wave assisted sequential extraction of water and dilute acid soluble arsemic species from marine plant and animal tissues

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    Abstract

    This paper describes the use of dilute nitric acid for the extraction and quantification of arsenic species. A number of extractants (e.g. water, 1.5 M orthophosphoric acid, methanol–water and dilute nitric acid) were tested for the extraction of arsenic from marine biological samples, such as plants that have proved difficult to quantitatively extract. Dilute 2% (v/v) nitric acid was found to give the highest recoveries of arsenic overall and was chosen for further optimisation. The optimal extraction conditions for arsenic were 2% (v/v) HNO3, 6 min−1, 90 °C. Arsenic species were found to be stable under the optimised conditions with the exception of the arsenoriboses which degraded to a product eluting at the same retention time as glycerol arsenoribose. Good agreement was found between the 2% (v/v) HNO3 extraction and the methanol–water extraction for the certified reference material DORM-2 (AB 17.1 and 16.2 μg g−1, respectively, and TETRA 0.27 and 0.25 μg g−1, respectively), which were in close agreement with the certified concentrations of AB 16.4 ± 1.1 μg g−1 and TETRA 0.248 ± 0.054 μg g−1. To preserve the integrity of arsenic species, a sequential extraction technique was developed where the previously methanol–water extracted pellet was further extracted with 2% (v/v) HNO3 under the optimised conditions. Increases in arsenic recoveries between 13% and 36% were found and speciation of this faction revealed that only inorganic and simple methylated species were extracted
    Original languageEnglish
    Pages (from-to)537-549
    Number of pages13
    JournalTalanta
    Volume71
    Issue number2
    DOIs
    Publication statusPublished - 2007

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    Arsenic
    Animals
    Tissue
    Acids
    Water
    Nitric Acid
    Recovery
    Glycerol

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    @article{7e9ba3f5b97549ec8d68d6d9341e3ede,
    title = "A micro-wave assisted sequential extraction of water and dilute acid soluble arsemic species from marine plant and animal tissues",
    abstract = "This paper describes the use of dilute nitric acid for the extraction and quantification of arsenic species. A number of extractants (e.g. water, 1.5 M orthophosphoric acid, methanol–water and dilute nitric acid) were tested for the extraction of arsenic from marine biological samples, such as plants that have proved difficult to quantitatively extract. Dilute 2{\%} (v/v) nitric acid was found to give the highest recoveries of arsenic overall and was chosen for further optimisation. The optimal extraction conditions for arsenic were 2{\%} (v/v) HNO3, 6 min−1, 90 °C. Arsenic species were found to be stable under the optimised conditions with the exception of the arsenoriboses which degraded to a product eluting at the same retention time as glycerol arsenoribose. Good agreement was found between the 2{\%} (v/v) HNO3 extraction and the methanol–water extraction for the certified reference material DORM-2 (AB 17.1 and 16.2 μg g−1, respectively, and TETRA 0.27 and 0.25 μg g−1, respectively), which were in close agreement with the certified concentrations of AB 16.4 ± 1.1 μg g−1 and TETRA 0.248 ± 0.054 μg g−1. To preserve the integrity of arsenic species, a sequential extraction technique was developed where the previously methanol–water extracted pellet was further extracted with 2{\%} (v/v) HNO3 under the optimised conditions. Increases in arsenic recoveries between 13{\%} and 36{\%} were found and speciation of this faction revealed that only inorganic and simple methylated species were extracted",
    author = "Simon Foster and Bill Maher and Simon Apte",
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    language = "English",
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    A micro-wave assisted sequential extraction of water and dilute acid soluble arsemic species from marine plant and animal tissues. / Foster, Simon; Maher, Bill; Apte, Simon.

    In: Talanta, Vol. 71, No. 2, 2007, p. 537-549.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A micro-wave assisted sequential extraction of water and dilute acid soluble arsemic species from marine plant and animal tissues

    AU - Foster, Simon

    AU - Maher, Bill

    AU - Apte, Simon

    PY - 2007

    Y1 - 2007

    N2 - This paper describes the use of dilute nitric acid for the extraction and quantification of arsenic species. A number of extractants (e.g. water, 1.5 M orthophosphoric acid, methanol–water and dilute nitric acid) were tested for the extraction of arsenic from marine biological samples, such as plants that have proved difficult to quantitatively extract. Dilute 2% (v/v) nitric acid was found to give the highest recoveries of arsenic overall and was chosen for further optimisation. The optimal extraction conditions for arsenic were 2% (v/v) HNO3, 6 min−1, 90 °C. Arsenic species were found to be stable under the optimised conditions with the exception of the arsenoriboses which degraded to a product eluting at the same retention time as glycerol arsenoribose. Good agreement was found between the 2% (v/v) HNO3 extraction and the methanol–water extraction for the certified reference material DORM-2 (AB 17.1 and 16.2 μg g−1, respectively, and TETRA 0.27 and 0.25 μg g−1, respectively), which were in close agreement with the certified concentrations of AB 16.4 ± 1.1 μg g−1 and TETRA 0.248 ± 0.054 μg g−1. To preserve the integrity of arsenic species, a sequential extraction technique was developed where the previously methanol–water extracted pellet was further extracted with 2% (v/v) HNO3 under the optimised conditions. Increases in arsenic recoveries between 13% and 36% were found and speciation of this faction revealed that only inorganic and simple methylated species were extracted

    AB - This paper describes the use of dilute nitric acid for the extraction and quantification of arsenic species. A number of extractants (e.g. water, 1.5 M orthophosphoric acid, methanol–water and dilute nitric acid) were tested for the extraction of arsenic from marine biological samples, such as plants that have proved difficult to quantitatively extract. Dilute 2% (v/v) nitric acid was found to give the highest recoveries of arsenic overall and was chosen for further optimisation. The optimal extraction conditions for arsenic were 2% (v/v) HNO3, 6 min−1, 90 °C. Arsenic species were found to be stable under the optimised conditions with the exception of the arsenoriboses which degraded to a product eluting at the same retention time as glycerol arsenoribose. Good agreement was found between the 2% (v/v) HNO3 extraction and the methanol–water extraction for the certified reference material DORM-2 (AB 17.1 and 16.2 μg g−1, respectively, and TETRA 0.27 and 0.25 μg g−1, respectively), which were in close agreement with the certified concentrations of AB 16.4 ± 1.1 μg g−1 and TETRA 0.248 ± 0.054 μg g−1. To preserve the integrity of arsenic species, a sequential extraction technique was developed where the previously methanol–water extracted pellet was further extracted with 2% (v/v) HNO3 under the optimised conditions. Increases in arsenic recoveries between 13% and 36% were found and speciation of this faction revealed that only inorganic and simple methylated species were extracted

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