A mouse partially protective glycoprotein antigen in Streptococcus agalactiae serotype Ia

Shawn Mark Somerset, G. H.G. Davis

Research output: Contribution to journalArticle

Abstract

The immunological properties of a glycoprotein antigen (antigen 2) of Streptococcus agalactiae serotype Ia were investigated. A specific antiserum was prepared by immunizing rabbits with antigen 2 immunoprecipitates excised from Crossed immunoelectrophoresis (Crossed IEP) gels. This antiserum produced a single peak representing antigen 2 when reacted with a Triton X-100 sonicate of heat-killed whole serotype Ia cells in Crossed IEP analysis. With polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and subsequent immunoelectroblotting, three strongly reacting polypeptides were detected at 60, 56, and 35 kilo-Daltons. Many faintly reacting polypeptides were detected between 67 and 30 kilodalton. The specific anti-antigen 2 serum used in Crossed immunoisoelectric focusing (XIEF) detected three immunoprecipitates, two with a pI of 8.4 and one with a pI of 6.7. Identification of the antigens detected in XIEF with the polypeptides detected by immunoelectroblotting was not attempted. The specific anti-antigen 2 serum partially protected mice against lethal serotype Ia infection.

LanguageEnglish
Pages213-216
Number of pages4
JournalCurrent Microbiology
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Jul 1986
Externally publishedYes

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Streptococcus agalactiae
Glycoproteins
Antigens
Two-Dimensional Immunoelectrophoresis
Immunoblotting
Peptides
Immune Sera
Octoxynol
Serogroup
Serum
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Hot Temperature
Gels
Rabbits
Infection

Cite this

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abstract = "The immunological properties of a glycoprotein antigen (antigen 2) of Streptococcus agalactiae serotype Ia were investigated. A specific antiserum was prepared by immunizing rabbits with antigen 2 immunoprecipitates excised from Crossed immunoelectrophoresis (Crossed IEP) gels. This antiserum produced a single peak representing antigen 2 when reacted with a Triton X-100 sonicate of heat-killed whole serotype Ia cells in Crossed IEP analysis. With polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and subsequent immunoelectroblotting, three strongly reacting polypeptides were detected at 60, 56, and 35 kilo-Daltons. Many faintly reacting polypeptides were detected between 67 and 30 kilodalton. The specific anti-antigen 2 serum used in Crossed immunoisoelectric focusing (XIEF) detected three immunoprecipitates, two with a pI of 8.4 and one with a pI of 6.7. Identification of the antigens detected in XIEF with the polypeptides detected by immunoelectroblotting was not attempted. The specific anti-antigen 2 serum partially protected mice against lethal serotype Ia infection.",
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A mouse partially protective glycoprotein antigen in Streptococcus agalactiae serotype Ia. / Somerset, Shawn Mark; Davis, G. H.G.

In: Current Microbiology, Vol. 14, No. 4, 01.07.1986, p. 213-216.

Research output: Contribution to journalArticle

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