A muscarinic cholinergic antagonist and a dopamine agonist rapidly increase ZENK mRNA expression in the form-deprived chicken retina

Regan Ashby, C. Siobhan McCarthy, Ryszard Maleszka, Pamela MEGAW, Ian G. Morgan

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    Increases in the expression of the immediate early gene ZENK in the retina, measured by changes in the levels of mRNA and protein immunoreactivity, are amongst the most rapid responses so far measured to conditions that decrease the rate of eye growth in chickens. Our aim was to determine whether atropine, a muscarinic cholinergic antagonist, and 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide, a dopamine agonist, which are known to block excessive eye growth, produce similar changes in ZENK expression. Form-deprivation resulted in significant down-regulation of the expression of retinal ZENK mRNA within 1 h of fitting the diffusers, whereas removal of the diffusers from the eyes of chickens that had developed form-deprivation myopia resulted in significant up-regulation of retinal ZENK expression within 1 h. When atropine (10 μL of 25 mM solution) and 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide (10 μL of a 10 mM solution) were injected intravitreally, just prior to fitting the diffusers, the down-regulation of retinal ZENK mRNA caused by form-deprivation was reversed. This resulted in levels of ZENK mRNA higher than in control or contralateral control eyes. The doses were chosen because they are known to block the excessive axial elongation induced by form-deprivation, without affecting the growth of control eyes. Neither agent had any effect on retinal ZENK expression within this time period when injected into control eyes. These results suggest that both muscarinic acetylcholine antagonists and dopamine agonists act early in the signal cascade controlling eye growth, possibly within the retina itself.

    Original languageEnglish
    Pages (from-to)15-22
    Number of pages8
    JournalExperimental Eye Research
    Volume85
    Issue number1
    DOIs
    Publication statusPublished - Jul 2007

    Fingerprint

    Muscarinic Antagonists
    Dopamine Agonists
    Retina
    Chickens
    Messenger RNA
    Growth
    Atropine
    Down-Regulation
    Cholinergic Agonists
    Immediate-Early Genes
    Myopia
    Cholinergic Antagonists
    Up-Regulation

    Cite this

    Ashby, Regan ; McCarthy, C. Siobhan ; Maleszka, Ryszard ; MEGAW, Pamela ; Morgan, Ian G. / A muscarinic cholinergic antagonist and a dopamine agonist rapidly increase ZENK mRNA expression in the form-deprived chicken retina. In: Experimental Eye Research. 2007 ; Vol. 85, No. 1. pp. 15-22.
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    A muscarinic cholinergic antagonist and a dopamine agonist rapidly increase ZENK mRNA expression in the form-deprived chicken retina. / Ashby, Regan; McCarthy, C. Siobhan; Maleszka, Ryszard; MEGAW, Pamela; Morgan, Ian G.

    In: Experimental Eye Research, Vol. 85, No. 1, 07.2007, p. 15-22.

    Research output: Contribution to journalArticle

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