A random PCR screening system for the identification of type 1 human herpes simplex virus

Xuelian Yu, Bisheng Shi, Yan Gong, Xiaonan Zhang, Silan Shen, Fangxing Qian, Shimin Gu, Yunwen Hu, Zhenghong Yuan

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases.

Original languageEnglish
Pages (from-to)91-7
Number of pages7
JournalJournal of Virological Methods
Volume161
Issue number1
DOIs
Publication statusPublished - Oct 2009

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