A random PCR screening system for the identification of type 1 human herpes simplex virus

Xuelian Yu; Bisheng Shi; Yan Gong; Xiaonan Zhang; Silan Shen; Fangxing Qian; Shimin Gu; Yunwen Hu; Zhenghong Yuan

doi:10.1016/j.jviromet.2009.05.023

A random PCR screening system for the identification of type 1 human herpes simplex virus

Xuelian Yu, Bisheng Shi, Yan Gong, Xiaonan Zhang, Silan Shen, Fangxing Qian, Shimin Gu, Yunwen Hu, Zhenghong Yuan

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases.

Original language	English
Pages (from-to)	91-7
Number of pages	7
Journal	Journal of Virological Methods
Volume	161
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2009.05.023
Publication status	Published - Oct 2009

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10.1016/j.jviromet.2009.05.023

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title = "A random PCR screening system for the identification of type 1 human herpes simplex virus",

abstract = "Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases.",

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author = "Xuelian Yu and Bisheng Shi and Yan Gong and Xiaonan Zhang and Silan Shen and Fangxing Qian and Shimin Gu and Yunwen Hu and Zhenghong Yuan",

year = "2009",

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doi = "10.1016/j.jviromet.2009.05.023",

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T1 - A random PCR screening system for the identification of type 1 human herpes simplex virus

AU - Yu, Xuelian

AU - Shi, Bisheng

AU - Gong, Yan

AU - Zhang, Xiaonan

AU - Shen, Silan

AU - Qian, Fangxing

AU - Gu, Shimin

AU - Hu, Yunwen

AU - Yuan, Zhenghong

PY - 2009/10

Y1 - 2009/10

N2 - Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases.

AB - Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases.

KW - Adult

KW - Female

KW - Herpes Simplex/diagnosis

KW - Herpesvirus 1, Human/isolation & purification

KW - Humans

KW - Male

KW - Mass Screening/methods

KW - Polymerase Chain Reaction/methods

KW - Young Adult

U2 - 10.1016/j.jviromet.2009.05.023

DO - 10.1016/j.jviromet.2009.05.023

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SP - 91

EP - 97

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

A random PCR screening system for the identification of type 1 human herpes simplex virus

Abstract

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