A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies

June Liu, P Kerr, Tanja Strive

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.
    Original languageEnglish
    Pages (from-to)1-5
    Number of pages5
    JournalVirology Journal
    Volume9
    DOIs
    Publication statusPublished - 2012

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    Enzyme-Linked Immunosorbent Assay
    Rabbit Haemorrhagic Disease Virus
    Rabbits
    Antibodies
    Viruses
    Serologic Tests
    Serum
    New Zealand
    Population
    Epidemiologic Studies
    Epidemiology
    Infection

    Cite this

    @article{52d2bd932ccb4024bb4680a329db9874,
    title = "A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies",
    abstract = "Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100{\%} sensitivity and no cross-reactivity with RHDV sera (100{\%} specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.",
    author = "June Liu and P Kerr and Tanja Strive",
    year = "2012",
    doi = "10.1186/1743-422X-9-182",
    language = "English",
    volume = "9",
    pages = "1--5",
    journal = "Virology Journal",
    issn = "1743-422X",
    publisher = "BioMed Central",

    }

    A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies. / Liu, June; Kerr, P; Strive, Tanja.

    In: Virology Journal, Vol. 9, 2012, p. 1-5.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies

    AU - Liu, June

    AU - Kerr, P

    AU - Strive, Tanja

    PY - 2012

    Y1 - 2012

    N2 - Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.

    AB - Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.

    U2 - 10.1186/1743-422X-9-182

    DO - 10.1186/1743-422X-9-182

    M3 - Article

    VL - 9

    SP - 1

    EP - 5

    JO - Virology Journal

    JF - Virology Journal

    SN - 1743-422X

    ER -