A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies

June Liu, P Kerr, Tanja Strive

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.
Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalVirology Journal
Volume9
DOIs
Publication statusPublished - 2012

Fingerprint

Enzyme-Linked Immunosorbent Assay
Rabbit Haemorrhagic Disease Virus
Rabbits
Antibodies
Viruses
Serologic Tests
Serum
New Zealand
Population
Epidemiologic Studies
Epidemiology
Infection

Cite this

@article{52d2bd932ccb4024bb4680a329db9874,
title = "A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies",
abstract = "Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100{\%} sensitivity and no cross-reactivity with RHDV sera (100{\%} specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.",
author = "June Liu and P Kerr and Tanja Strive",
year = "2012",
doi = "10.1186/1743-422X-9-182",
language = "English",
volume = "9",
pages = "1--5",
journal = "Virology Journal",
issn = "1743-422X",
publisher = "BioMed Central",

}

A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies. / Liu, June; Kerr, P; Strive, Tanja.

In: Virology Journal, Vol. 9, 2012, p. 1-5.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies

AU - Liu, June

AU - Kerr, P

AU - Strive, Tanja

PY - 2012

Y1 - 2012

N2 - Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.

AB - Background Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia. Results A highly sensitive and specific blocking ELISA was developed for the detection of RCV-A1 antibodies. When sera from rabbits with a known infection history for either RCV-A1 or RHDV were tested, this assay showed 100% sensitivity and no cross-reactivity with RHDV sera (100% specificity). Conclusions This new ELISA not only allows the detection of RCV-A1 at a population level, but also permits the serological status of individual rabbits to be determined more reliably than previously described methods. This robust and simple to perform assay is therefore the tool of choice for studying RCV-A1 epidemiology in Australian wild rabbit populations.

U2 - 10.1186/1743-422X-9-182

DO - 10.1186/1743-422X-9-182

M3 - Article

VL - 9

SP - 1

EP - 5

JO - Virology Journal

JF - Virology Journal

SN - 1743-422X

ER -