A simple method for in vivo protein labeling in intact rice plant anthers: Water stress effects on protein synthesis

Three methods for in vivo protein labelling of rice anthers were tested. The ‘wick’ method was found to incorporate 5 - and 9- fold higher amount of labelled 35S-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use with any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis for the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.