A simple method for in vivo protein labeling in intact rice plant anthers

Water stress effects on protein synthesis

Deep Saini, Inder S. Sheoran

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three methods for in vivo protein labelling of rice anthers were tested. The ‘wick’ method was found to incorporate 5 - and 9- fold higher amount of labelled 35S-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use with any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis for the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.
Original languageEnglish
Pages (from-to)47-50
Number of pages4
JournalJournal of Plant Biochemistry and Biotechnology
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1998
Externally publishedYes

Fingerprint

Dehydration
anthers
water stress
protein synthesis
rice
Proteins
Capillary Action
methodology
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
Methionine
reproducibility
polyacrylamide gel electrophoresis
methionine
proteins
Oryza
testing

Cite this

@article{dd465251ad4a45bfa6ca8a367752cab4,
title = "A simple method for in vivo protein labeling in intact rice plant anthers: Water stress effects on protein synthesis",
abstract = "Three methods for in vivo protein labelling of rice anthers were tested. The ‘wick’ method was found to incorporate 5 - and 9- fold higher amount of labelled 35S-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use with any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis for the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.",
keywords = "Anther, In vivo, Protein synthesis, Rice, Water stress",
author = "Deep Saini and Sheoran, {Inder S.}",
year = "1998",
month = "1",
doi = "10.1007/BF03263033",
language = "English",
volume = "7",
pages = "47--50",
journal = "Journal of Plant Biochemistry and Biotechnology",
issn = "0971-7811",
publisher = "Springer",
number = "1",

}

TY - JOUR

T1 - A simple method for in vivo protein labeling in intact rice plant anthers

T2 - Water stress effects on protein synthesis

AU - Saini, Deep

AU - Sheoran, Inder S.

PY - 1998/1

Y1 - 1998/1

N2 - Three methods for in vivo protein labelling of rice anthers were tested. The ‘wick’ method was found to incorporate 5 - and 9- fold higher amount of labelled 35S-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use with any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis for the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.

AB - Three methods for in vivo protein labelling of rice anthers were tested. The ‘wick’ method was found to incorporate 5 - and 9- fold higher amount of labelled 35S-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use with any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis for the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.

KW - Anther

KW - In vivo

KW - Protein synthesis

KW - Rice

KW - Water stress

UR - http://www.scopus.com/inward/record.url?scp=0031803691&partnerID=8YFLogxK

U2 - 10.1007/BF03263033

DO - 10.1007/BF03263033

M3 - Article

VL - 7

SP - 47

EP - 50

JO - Journal of Plant Biochemistry and Biotechnology

JF - Journal of Plant Biochemistry and Biotechnology

SN - 0971-7811

IS - 1

ER -