A Simple non-invasive protocol to establish primary cell lines from tail and toe implants for cytogenic studies in Australian dragon lizards (Squamata: Adamidae)

Tariq Ezaz, Denis O'Meally, Alex Quinn, Stephen Sarre, Arthur Georges, Jenny Marshall Graves

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    13 Citations (Scopus)

    Abstract

    Primary cell lines were established from cultures of tail and toe clips of five species of Australian dragon lizards: Tympanocryptis pinguicolla, Tympanocryptis sp., Ctenophorus fordi, Amphibolurus norrisi and Pogona vitticeps. The start of exponential cell growth ranged from 1 to 5 weeks. Cultures from all specimens had fibroblastic morphology. Cell lines were propagated continuously up to ten passages, cryopreserved and recovered successfully. We found no reduction in cell viability after short term (<6 months) storage at −80 °C. Mitotic metaphase chromosomes were harvested from these cell lines and used in differential staining, banding and fluorescent in situ hybridisation. Cell lines maintained normal diploidy in all species. This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice. The method is likely to be applicable to a range of species. Such cell lines provide a virtually unlimited source of material for cytogenetic, evolutionary and genomic studies
    Original languageEnglish
    Pages (from-to)135-139
    Number of pages5
    JournalEuropean Journal of Innovation Management
    Volume58
    Issue number3
    DOIs
    Publication statusPublished - 2008

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    Cells
    Cell culture
    Cell growth
    Chromosomes
    Cell line
    Implant

    Cite this

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    abstract = "Primary cell lines were established from cultures of tail and toe clips of five species of Australian dragon lizards: Tympanocryptis pinguicolla, Tympanocryptis sp., Ctenophorus fordi, Amphibolurus norrisi and Pogona vitticeps. The start of exponential cell growth ranged from 1 to 5 weeks. Cultures from all specimens had fibroblastic morphology. Cell lines were propagated continuously up to ten passages, cryopreserved and recovered successfully. We found no reduction in cell viability after short term (<6 months) storage at −80 °C. Mitotic metaphase chromosomes were harvested from these cell lines and used in differential staining, banding and fluorescent in situ hybridisation. Cell lines maintained normal diploidy in all species. This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice. The method is likely to be applicable to a range of species. Such cell lines provide a virtually unlimited source of material for cytogenetic, evolutionary and genomic studies",
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    AU - Ezaz, Tariq

    AU - O'Meally, Denis

    AU - Quinn, Alex

    AU - Sarre, Stephen

    AU - Georges, Arthur

    AU - Marshall Graves, Jenny

    PY - 2008

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