A unique T cell receptor discovered in marsupials

Z.E. Parra, M.L. Baker, R.S. Schwarz, J.E. Deakin, K. Lindblad-Toh, R.D. Miller

    Research output: Contribution to journalArticle

    63 Citations (Scopus)

    Abstract

    T cells recognize antigens by using T cell receptors (TCRs) encoded by gene segments, called variable (V), diversity (D), and joining (J), that undergo somatic recombination to create diverse binding specificities. Four TCR chains (α, β, γ, and δ) have been identified to date, and, as T cells develop in the thymus, they express exclusively either an αβTCR or a γδTCR heterodimer. Here, we show that marsupials have an additional TCR (TCRμ) that has V, D, and J that are either somatically recombined, as in conventional TCRs, or are already prejoined in the germ-line DNA in a manner consistent with their creation by retrotransposition. TCRμ, does not have a known homolog in eutherian mammals but has features analogous to a recently described TCRδ isoform in sharks. TCRμ is expressed in at least two mRNA isoforms that appear capable of encoding a full-length protein, both of which are transcribed in the thymus and spleen. One contains two variable domains: a somatically recombined V and a prejoined V. This appears to be the dominant isoform in peripheral lymphoid tissue. The other isoform contains only the prejoined V and is structurally more similar to conventional TCR chains, however invariant. Unlike other TCRs, TCRμ, uses prejoined gene segments and is likely present in all marsupials. Its similarity to a TCR isoform in sharks suggests that it, or something similar, may be present in other vertebrate lineages and, therefore, may represent an ancient receptor system.
    Original languageUndefined
    Pages (from-to)9776-9781
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume104
    Issue number23
    DOIs
    Publication statusPublished - 2007

    Cite this

    Parra, Z.E. ; Baker, M.L. ; Schwarz, R.S. ; Deakin, J.E. ; Lindblad-Toh, K. ; Miller, R.D. / A unique T cell receptor discovered in marsupials. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 23. pp. 9776-9781.
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    abstract = "T cells recognize antigens by using T cell receptors (TCRs) encoded by gene segments, called variable (V), diversity (D), and joining (J), that undergo somatic recombination to create diverse binding specificities. Four TCR chains (α, β, γ, and δ) have been identified to date, and, as T cells develop in the thymus, they express exclusively either an αβTCR or a γδTCR heterodimer. Here, we show that marsupials have an additional TCR (TCRμ) that has V, D, and J that are either somatically recombined, as in conventional TCRs, or are already prejoined in the germ-line DNA in a manner consistent with their creation by retrotransposition. TCRμ, does not have a known homolog in eutherian mammals but has features analogous to a recently described TCRδ isoform in sharks. TCRμ is expressed in at least two mRNA isoforms that appear capable of encoding a full-length protein, both of which are transcribed in the thymus and spleen. One contains two variable domains: a somatically recombined V and a prejoined V. This appears to be the dominant isoform in peripheral lymphoid tissue. The other isoform contains only the prejoined V and is structurally more similar to conventional TCR chains, however invariant. Unlike other TCRs, TCRμ, uses prejoined gene segments and is likely present in all marsupials. Its similarity to a TCR isoform in sharks suggests that it, or something similar, may be present in other vertebrate lineages and, therefore, may represent an ancient receptor system.",
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    A unique T cell receptor discovered in marsupials. / Parra, Z.E.; Baker, M.L.; Schwarz, R.S.; Deakin, J.E.; Lindblad-Toh, K.; Miller, R.D.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 23, 2007, p. 9776-9781.

    Research output: Contribution to journalArticle

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    AU - Parra, Z.E.

    AU - Baker, M.L.

    AU - Schwarz, R.S.

    AU - Deakin, J.E.

    AU - Lindblad-Toh, K.

    AU - Miller, R.D.

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