TY - JOUR
T1 - Against the mainstream
T2 - exceptional evolutionary stability of ZW sex chromosomes across the fish families Triportheidae and Gasteropelecidae (Teleostei: Characiformes)
AU - Yano, Cassia Fernanda
AU - Sember, Alexandr
AU - Kretschmer, Rafael
AU - Bertollo, Luiz Antônio Carlos
AU - Ezaz, Tariq
AU - Hatanaka, Terumi
AU - Liehr, Thomas
AU - Ráb, Petr
AU - Al-Rikabi, Ahmed
AU - Viana, Patrik Ferreira
AU - Feldberg, Eliana
AU - de Oliveira, Ezequiel Aguiar
AU - Toma, Gustavo Akira
AU - de Bello Cioffi, Marcelo
N1 - Funding Information:
Marcelo de Bello Cioffi was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Proc. No. 302449/2018–3) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Proc. No. 2020/11772–8). Thomas Liehr was supported by DAAD/ PROPASP/ FAPESP (Proc. No. 2019/04877–0). Rafael Kretschmer was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Proc. No. 2020/11669–2). Gustavo Akira Tomas was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Proc. No. 2018/14677–6). Alexandr Sember and Petr Ráb were supported by RVO: 67985904 of IAPG CAS, Liběchov.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2021/12
Y1 - 2021/12
N2 - Teleost fishes exhibit a breath-taking diversity of sex determination and differentiation mechanisms. They encompass at least nine sex chromosome systems with often low degree of differentiation, high rate of inter- and intra-specific variability, and frequent turnovers. Nevertheless, several mainly female heterogametic systems at an advanced stage of genetic differentiation and high evolutionary stability have been also found across teleosts, especially among Neotropical characiforms. In this study, we aim to characterize the ZZ/ZW sex chromosome system in representatives of the Triportheidae family (Triportheus auritus, Agoniates halecinus, and the basal-most species Lignobrycon myersi) and its sister clade Gasteropelecidae (Carnegiella strigata, Gasteropelecus levis, and Thoracocharax stellatus). We applied both conventional and molecular cytogenetic approaches including chromosomal mapping of 5S and 18S ribosomal DNA clusters, cross-species chromosome painting (Zoo-FISH) with sex chromosome-derived probes and comparative genomic hybridization (CGH). We identified the ZW sex chromosome system for the first time in A. halecinus and G. levis and also in C. strigata formerly reported to lack sex chromosomes. We also brought evidence for possible mechanisms underlying the sex chromosome differentiation, including inversions, repetitive DNA accumulation, and exchange of genetic material. Our Zoo-FISH experiments further strongly indicated that the ZW sex chromosomes of Triportheidae and Gasteropelecidae are homeologous, suggesting their origin before the split of these lineages (approx. 40–70 million years ago). Such extent of sex chromosome stability is almost exceptional in teleosts, and hence, these lineages afford a special opportunity to scrutinize unique evolutionary forces and pressures shaping sex chromosome evolution in fishes and vertebrates in general.
AB - Teleost fishes exhibit a breath-taking diversity of sex determination and differentiation mechanisms. They encompass at least nine sex chromosome systems with often low degree of differentiation, high rate of inter- and intra-specific variability, and frequent turnovers. Nevertheless, several mainly female heterogametic systems at an advanced stage of genetic differentiation and high evolutionary stability have been also found across teleosts, especially among Neotropical characiforms. In this study, we aim to characterize the ZZ/ZW sex chromosome system in representatives of the Triportheidae family (Triportheus auritus, Agoniates halecinus, and the basal-most species Lignobrycon myersi) and its sister clade Gasteropelecidae (Carnegiella strigata, Gasteropelecus levis, and Thoracocharax stellatus). We applied both conventional and molecular cytogenetic approaches including chromosomal mapping of 5S and 18S ribosomal DNA clusters, cross-species chromosome painting (Zoo-FISH) with sex chromosome-derived probes and comparative genomic hybridization (CGH). We identified the ZW sex chromosome system for the first time in A. halecinus and G. levis and also in C. strigata formerly reported to lack sex chromosomes. We also brought evidence for possible mechanisms underlying the sex chromosome differentiation, including inversions, repetitive DNA accumulation, and exchange of genetic material. Our Zoo-FISH experiments further strongly indicated that the ZW sex chromosomes of Triportheidae and Gasteropelecidae are homeologous, suggesting their origin before the split of these lineages (approx. 40–70 million years ago). Such extent of sex chromosome stability is almost exceptional in teleosts, and hence, these lineages afford a special opportunity to scrutinize unique evolutionary forces and pressures shaping sex chromosome evolution in fishes and vertebrates in general.
KW - CGH
KW - chromosome rearrangements
KW - rDNA
KW - sex chromosome evolution
KW - Zoo-FISH
UR - http://www.scopus.com/inward/record.url?scp=85117772089&partnerID=8YFLogxK
U2 - 10.1007/s10577-021-09674-1
DO - 10.1007/s10577-021-09674-1
M3 - Article
C2 - 34694531
AN - SCOPUS:85117772089
SN - 0967-3849
VL - 29
SP - 391
EP - 416
JO - Chromosome Research
JF - Chromosome Research
IS - 3-4
ER -