Alterations in acute-phase reactants (CRP, rheumatoid factor, complement, Factor B, and immune complexes) following an ultramarathon

SJ Semple, LL Smith, AJ Mckune, N Neveling, Ahmed Wadee

Research output: Contribution to journalArticle

Abstract

Objectives. The human body initiates an acute phase response (APR) in response to a wide range of homeostatic disturbances. This complex series of reactions serves to activate repair processes and prevent ongoing tissue damage. An important aspect of the APR is the de novo synthesis of acute phase proteins (APP), many of which have not been thoroughly investigated. Main outcome measures. Alterations in CRP (C-reactive protein), C1est, C3, C4, C6, rheumatoid factor (RF) and Factor B were determined before and after an ultramarathon. Data were analysed using a one-way analysis of variance comparing values to pre-exercise levels. Significance was set at p < 0.05. Design. Venepunctures were performed on athletes participating in an ultramarathon (90 km) 24 hours before, immediately post-exercise (IPE), and 3h, 24h and 72h after the race. Serum was stored at –80°C until analysed. CRP levels in serum were assessed using the N Latex CRP kit. The levels of circulating immune complexes (CIC) were determined using particle-enhanced nephelometry. Complement proteins C1est, C3, C4 and RF were measured using laser nephelometry. C6 and Factor B were determined by radial immunodiffusion. Results. CRP was significantly elevated IPE (58%), 3h post (77%), 24h post (87%) and 72h post (69%). Pre-race CRP levels were above the normative range (5.10 ± 3.08 mg/l), C6 was significantly elevated (p < 0.05) at 24h post (7.8%) and 72h post (8.8%) exercise. Factor B was significantly elevated (p < 0.05) at 72h post exercise (12.8%). RF was significantly elevated at 72h post exercise (6.7%). Conclusion. Significant increases in selected acutephase reactants occur several days after the exercise event. In addition, as indicated by elevated resting levels of CRP, the athletes began the race with some degree of inflammation, presumably as a result of the cumulative training and racing mileage in preparation for the ultramarathon.
Original languageEnglish
Pages (from-to)17, 21
JournalSouth African Journal of Sports Medicine
Volume16
Issue number2
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Complement Factor B
Acute-Phase Proteins
Rheumatoid Factor
Antigen-Antibody Complex
Exercise
Nephelometry and Turbidimetry
Athletes
Phlebotomy
Immunodiffusion
Serum
Human Body
Complement System Proteins
Lasers
Outcome Assessment (Health Care)

Cite this

@article{e13e8c9d8b3d419c8640dd88fd24e3d9,
title = "Alterations in acute-phase reactants (CRP, rheumatoid factor, complement, Factor B, and immune complexes) following an ultramarathon",
abstract = "Objectives. The human body initiates an acute phase response (APR) in response to a wide range of homeostatic disturbances. This complex series of reactions serves to activate repair processes and prevent ongoing tissue damage. An important aspect of the APR is the de novo synthesis of acute phase proteins (APP), many of which have not been thoroughly investigated. Main outcome measures. Alterations in CRP (C-reactive protein), C1est, C3, C4, C6, rheumatoid factor (RF) and Factor B were determined before and after an ultramarathon. Data were analysed using a one-way analysis of variance comparing values to pre-exercise levels. Significance was set at p < 0.05. Design. Venepunctures were performed on athletes participating in an ultramarathon (90 km) 24 hours before, immediately post-exercise (IPE), and 3h, 24h and 72h after the race. Serum was stored at –80°C until analysed. CRP levels in serum were assessed using the N Latex CRP kit. The levels of circulating immune complexes (CIC) were determined using particle-enhanced nephelometry. Complement proteins C1est, C3, C4 and RF were measured using laser nephelometry. C6 and Factor B were determined by radial immunodiffusion. Results. CRP was significantly elevated IPE (58{\%}), 3h post (77{\%}), 24h post (87{\%}) and 72h post (69{\%}). Pre-race CRP levels were above the normative range (5.10 ± 3.08 mg/l), C6 was significantly elevated (p < 0.05) at 24h post (7.8{\%}) and 72h post (8.8{\%}) exercise. Factor B was significantly elevated (p < 0.05) at 72h post exercise (12.8{\%}). RF was significantly elevated at 72h post exercise (6.7{\%}). Conclusion. Significant increases in selected acutephase reactants occur several days after the exercise event. In addition, as indicated by elevated resting levels of CRP, the athletes began the race with some degree of inflammation, presumably as a result of the cumulative training and racing mileage in preparation for the ultramarathon.",
author = "SJ Semple and LL Smith and AJ Mckune and N Neveling and Ahmed Wadee",
year = "2004",
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language = "English",
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pages = "17, 21",
journal = "South African Journal of Sports Medicine",
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}

Alterations in acute-phase reactants (CRP, rheumatoid factor, complement, Factor B, and immune complexes) following an ultramarathon. / Semple, SJ; Smith, LL; Mckune, AJ; Neveling, N; Wadee, Ahmed.

In: South African Journal of Sports Medicine, Vol. 16, No. 2, 2004, p. 17, 21.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alterations in acute-phase reactants (CRP, rheumatoid factor, complement, Factor B, and immune complexes) following an ultramarathon

AU - Semple, SJ

AU - Smith, LL

AU - Mckune, AJ

AU - Neveling, N

AU - Wadee, Ahmed

PY - 2004

Y1 - 2004

N2 - Objectives. The human body initiates an acute phase response (APR) in response to a wide range of homeostatic disturbances. This complex series of reactions serves to activate repair processes and prevent ongoing tissue damage. An important aspect of the APR is the de novo synthesis of acute phase proteins (APP), many of which have not been thoroughly investigated. Main outcome measures. Alterations in CRP (C-reactive protein), C1est, C3, C4, C6, rheumatoid factor (RF) and Factor B were determined before and after an ultramarathon. Data were analysed using a one-way analysis of variance comparing values to pre-exercise levels. Significance was set at p < 0.05. Design. Venepunctures were performed on athletes participating in an ultramarathon (90 km) 24 hours before, immediately post-exercise (IPE), and 3h, 24h and 72h after the race. Serum was stored at –80°C until analysed. CRP levels in serum were assessed using the N Latex CRP kit. The levels of circulating immune complexes (CIC) were determined using particle-enhanced nephelometry. Complement proteins C1est, C3, C4 and RF were measured using laser nephelometry. C6 and Factor B were determined by radial immunodiffusion. Results. CRP was significantly elevated IPE (58%), 3h post (77%), 24h post (87%) and 72h post (69%). Pre-race CRP levels were above the normative range (5.10 ± 3.08 mg/l), C6 was significantly elevated (p < 0.05) at 24h post (7.8%) and 72h post (8.8%) exercise. Factor B was significantly elevated (p < 0.05) at 72h post exercise (12.8%). RF was significantly elevated at 72h post exercise (6.7%). Conclusion. Significant increases in selected acutephase reactants occur several days after the exercise event. In addition, as indicated by elevated resting levels of CRP, the athletes began the race with some degree of inflammation, presumably as a result of the cumulative training and racing mileage in preparation for the ultramarathon.

AB - Objectives. The human body initiates an acute phase response (APR) in response to a wide range of homeostatic disturbances. This complex series of reactions serves to activate repair processes and prevent ongoing tissue damage. An important aspect of the APR is the de novo synthesis of acute phase proteins (APP), many of which have not been thoroughly investigated. Main outcome measures. Alterations in CRP (C-reactive protein), C1est, C3, C4, C6, rheumatoid factor (RF) and Factor B were determined before and after an ultramarathon. Data were analysed using a one-way analysis of variance comparing values to pre-exercise levels. Significance was set at p < 0.05. Design. Venepunctures were performed on athletes participating in an ultramarathon (90 km) 24 hours before, immediately post-exercise (IPE), and 3h, 24h and 72h after the race. Serum was stored at –80°C until analysed. CRP levels in serum were assessed using the N Latex CRP kit. The levels of circulating immune complexes (CIC) were determined using particle-enhanced nephelometry. Complement proteins C1est, C3, C4 and RF were measured using laser nephelometry. C6 and Factor B were determined by radial immunodiffusion. Results. CRP was significantly elevated IPE (58%), 3h post (77%), 24h post (87%) and 72h post (69%). Pre-race CRP levels were above the normative range (5.10 ± 3.08 mg/l), C6 was significantly elevated (p < 0.05) at 24h post (7.8%) and 72h post (8.8%) exercise. Factor B was significantly elevated (p < 0.05) at 72h post exercise (12.8%). RF was significantly elevated at 72h post exercise (6.7%). Conclusion. Significant increases in selected acutephase reactants occur several days after the exercise event. In addition, as indicated by elevated resting levels of CRP, the athletes began the race with some degree of inflammation, presumably as a result of the cumulative training and racing mileage in preparation for the ultramarathon.

U2 - 10.17159/2078-516X/2004/v16i2a183

DO - 10.17159/2078-516X/2004/v16i2a183

M3 - Article

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SP - 17, 21

JO - South African Journal of Sports Medicine

JF - South African Journal of Sports Medicine

SN - 1015-5163

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