An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats

Ross McEwing, Andrew Kitchener, Clare HOLLELEY, Kerry Kilshaw, Paul O’Donoghue

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Here we present an allelic discrimination assay designed to distinguish European wildcat mtDNA lineages from those of the domestic cat. Introgression between the native wildcat and introduced domestic cat has the potential to limit and reduce the recent recovery of remnant populations. Applied conservation genetic techniques can aid current conservation decisions on lethal control and neutering measures in hybrid zones. This real-time PCR technique offers a rapid, inexpensive and reliable assay to assess mtDNA introgression in the wild and has already identified hybrid individuals in the Scottish wildcat captive breeding programme.
Original languageEnglish
Pages (from-to)163-165
Number of pages3
JournalConservation Genetics Resources
Volume4
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

introgression
Mitochondrial DNA
Single Nucleotide Polymorphism
Cats
mitochondrial DNA
assay
cats
Genetic Techniques
captive breeding
conservation genetics
hybrid zone
assays
germplasm conservation
castration
lethal genes
Breeding
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
breeding
methodology

Cite this

McEwing, Ross ; Kitchener, Andrew ; HOLLELEY, Clare ; Kilshaw, Kerry ; O’Donoghue, Paul. / An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats. In: Conservation Genetics Resources. 2012 ; Vol. 4. pp. 163-165.
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An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats. / McEwing, Ross; Kitchener, Andrew; HOLLELEY, Clare; Kilshaw, Kerry; O’Donoghue, Paul.

In: Conservation Genetics Resources, Vol. 4, 2012, p. 163-165.

Research output: Contribution to journalArticle

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