An environmental DNA-based method for monitoring spawning activity

a case study, using the endangered Macquarie perch (Macquaria australasica)

Elise FURLAN, Christopher Hardy, Prudence McGuffie, Mark LINTERMANS, Dianne GLEESON

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    31 Citations (Scopus)
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    Abstract

    Determining the timing and location of reproductive events is critical for efficient management of species. However, methods currently used for aquatic species are costly, time intensive, biased and often require destructive or injurious sampling. Hence, developing a non-invasive sampling method to accurately determine the timing and location of reproduction for aquatic species would be extremely valuable. We conducted an experimental and field study to determine the influence of spawning, and the mass release of spermatozoa in particular, on environmental DNA (eDNA) concentrations. Using a quantitative PCR approach we monitored changes in nuclear and mitochondrial eDNA concentrations over time. The data from the experimental study and the field survey supported our hypothesis that spawning events are characterized by higher concentrations of nuclear relative to mitochondrial eDNA. Outside of the reproductive period, we find that nuclear and mitochondrial DNA fragments are equally abundant in environmental water samples. We have shown that changes in the relative abundance of nuclear and mitochondrial eDNA can be used to monitor spawning activity of the endangered Macquarie perch. Our method is likely to be transferrable to other aquatic species and can be particularly useful to increase our understanding of the spawning biology of cryptic, rare or threatened species as well as design and evaluate environmental management actions and determine species establishment.

    Original languageEnglish
    Pages (from-to)646-655
    Number of pages10
    JournalMethods in Ecology and Evolution
    Volume8
    Issue number5
    DOIs
    Publication statusPublished - 2017

    Fingerprint

    Macquaria australasica
    spawning
    case studies
    DNA
    monitoring
    environmental management
    experimental study
    threatened species
    methodology
    sampling
    nuclear genome
    quantitative polymerase chain reaction
    mitochondrial DNA
    spermatozoa
    Biological Sciences
    field survey
    method
    relative abundance
    water

    Cite this

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    AU - McGuffie, Prudence

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    AU - GLEESON, Dianne

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