An evaluation of the use of reptile dermal scutes as a non-invasive method to monitor mercury concentrations in the environment

Sam Eggins, Bill MAHER, Richard C. Vogt, Les Kinsley, Stephen Eggins, R Da Silveira

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Reptiles are ideal organisms for the non-invasive monitoring of mercury (Hg) contamination. We have investigated Hg bioaccumulation in tissue layers of reptile dermis as a basis for establishing a standardized collection method for Hg analysis. Tissue samples from freshwater turtle species Podocnemis unifilis and Podocnemis expansa and caiman species Melanosuchus niger and Caiman crocodilus, all from the Amazonian region, were analysed in this study. We first tested the relationships between Hg concentrations in keratin and bone to Hg concentrations in muscle to determine the best predictor of Hg concentration in muscle tissue. We then investigated the potential for measuring Hg concentrations across turtle carapace growth rings as an indicator of longer term changes in Hg concentration in the environment. Hg concentrations were significantly lower in bone (120ngg-1 caimans and 1ngg-1 turtles) than keratin (3600ngg-1 caimans and 2200ngg-1 turtles). Keratin was found to be a better predictor of exposure to Hg than muscle and bone tissues for both turtles and caimans and also to be a reliable non-invasive tissue for Hg analysis in turtles. Measurement of Hg in carapace growth rings has significant potential for estimating Hg bioaccumulation by turtles over time, but full quantification awaits development and use of a matrix-matched reference material for laser ablation ICPMS analysis of Hg concentrations in keratin. Realising this potential would make a valuable advance to the study of the history of contamination in mining and industrial sites, which have until now relied on the analysis of Hg concentrations in sediments.
    Original languageEnglish
    Pages (from-to)163-170
    Number of pages8
    JournalChemosphere
    Volume119
    Issue number9
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Turtles
    Reptiles
    Mercury
    reptile
    turtle
    Alligators and Crocodiles
    Tissue
    Skin
    Keratins
    Muscle
    Animal Shells
    Bioaccumulation
    Bone
    bone
    muscle
    Contamination
    Keratin-1
    Bone and Bones
    Muscles
    bioaccumulation

    Cite this

    Eggins, Sam ; MAHER, Bill ; Vogt, Richard C. ; Kinsley, Les ; Eggins, Stephen ; Da Silveira, R. / An evaluation of the use of reptile dermal scutes as a non-invasive method to monitor mercury concentrations in the environment. In: Chemosphere. 2015 ; Vol. 119, No. 9. pp. 163-170.
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    abstract = "Reptiles are ideal organisms for the non-invasive monitoring of mercury (Hg) contamination. We have investigated Hg bioaccumulation in tissue layers of reptile dermis as a basis for establishing a standardized collection method for Hg analysis. Tissue samples from freshwater turtle species Podocnemis unifilis and Podocnemis expansa and caiman species Melanosuchus niger and Caiman crocodilus, all from the Amazonian region, were analysed in this study. We first tested the relationships between Hg concentrations in keratin and bone to Hg concentrations in muscle to determine the best predictor of Hg concentration in muscle tissue. We then investigated the potential for measuring Hg concentrations across turtle carapace growth rings as an indicator of longer term changes in Hg concentration in the environment. Hg concentrations were significantly lower in bone (120ngg-1 caimans and 1ngg-1 turtles) than keratin (3600ngg-1 caimans and 2200ngg-1 turtles). Keratin was found to be a better predictor of exposure to Hg than muscle and bone tissues for both turtles and caimans and also to be a reliable non-invasive tissue for Hg analysis in turtles. Measurement of Hg in carapace growth rings has significant potential for estimating Hg bioaccumulation by turtles over time, but full quantification awaits development and use of a matrix-matched reference material for laser ablation ICPMS analysis of Hg concentrations in keratin. Realising this potential would make a valuable advance to the study of the history of contamination in mining and industrial sites, which have until now relied on the analysis of Hg concentrations in sediments.",
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    An evaluation of the use of reptile dermal scutes as a non-invasive method to monitor mercury concentrations in the environment. / Eggins, Sam; MAHER, Bill; Vogt, Richard C.; Kinsley, Les; Eggins, Stephen; Da Silveira, R.

    In: Chemosphere, Vol. 119, No. 9, 2015, p. 163-170.

    Research output: Contribution to journalArticle

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    AU - MAHER, Bill

    AU - Vogt, Richard C.

    AU - Kinsley, Les

    AU - Eggins, Stephen

    AU - Da Silveira, R

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