An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus

Robyn Hall, Lorenzo Capucci, Markus Matthaei, Simona Esposito, Peter Kerr, Michael FRESE, Tanja Strive

Research output: Contribution to journalArticle

3 Citations (Scopus)
6 Downloads (Pure)

Abstract

The calicivirus Rabbit haemorrhagic disease virus (RHDV) is widely used in Australia as a biocontrol agent to manage wild European rabbit (Oryctolagus cuniculus) populations. However, widespread herd immunity limits the effectiveness of the currently used strain, CAPM V-351. To overcome this, we developed an experimental platform for the selection and characterisation of novel RHDV strains. As RHDV does not replicate in cell culture, variant viruses were selected by serially passaging a highly virulent RHDV field isolate in immunologically naïve laboratory rabbits that were passively immunised 18-24 hours post-challenge with a neutralising monoclonal antibody. After seven passages, two amino acid substitutions in the P2 domain of the capsid protein became fixed within the virus population. Furthermore, a synonymous substitution within the coding sequence of the viral polymerase appeared and was also maintained in all subsequent passages. These findings demonstrate proof-of-concept that RHDV evolution can be experimentally manipulated to select for virus variants with altered phenotypes, in this case partial immune escape.
Original languageEnglish
Article numbere0173727
Pages (from-to)1-15
Number of pages15
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 2017

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Rabbit Haemorrhagic Disease Virus
Rabbit hemorrhagic disease virus
Viruses
Rabbits
viruses
Vesivirus
rabbits
Herd Immunity
Oryctolagus cuniculus
Capsid Proteins
amino acid substitution
Amino Acid Substitution
Neutralizing Antibodies
coat proteins
Substitution reactions
neutralizing antibodies
Biocontrol
Population
biological control agents
monoclonal antibodies

Cite this

Hall, R., Capucci, L., Matthaei, M., Esposito, S., Kerr, P., FRESE, M., & Strive, T. (2017). An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus. PLoS One, 12(3), 1-15. [e0173727]. https://doi.org/10.1371/journal.pone.0173727
Hall, Robyn ; Capucci, Lorenzo ; Matthaei, Markus ; Esposito, Simona ; Kerr, Peter ; FRESE, Michael ; Strive, Tanja. / An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus. In: PLoS One. 2017 ; Vol. 12, No. 3. pp. 1-15.
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Hall, R, Capucci, L, Matthaei, M, Esposito, S, Kerr, P, FRESE, M & Strive, T 2017, 'An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus', PLoS One, vol. 12, no. 3, e0173727, pp. 1-15. https://doi.org/10.1371/journal.pone.0173727

An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus. / Hall, Robyn; Capucci, Lorenzo; Matthaei, Markus; Esposito, Simona; Kerr, Peter ; FRESE, Michael; Strive, Tanja.

In: PLoS One, Vol. 12, No. 3, e0173727, 03.2017, p. 1-15.

Research output: Contribution to journalArticle

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AU - Capucci, Lorenzo

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AU - Strive, Tanja

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