Analysis of lipid peroxidation in human spermatozoa using BODIPY C11

R. John Aitken, Jordana K. Wingate, Geoffry N. De Iuliis, Eileen A. McLaughlin

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Lipid peroxidation is known to be a major factor in the aetiology of defective sperm function. Although biochemical assays for this process exist, they are relatively insensitive and require large numbers of spermatozoa; a condition that cannot be met with many infertility specimens. Recently, a new approach for monitoring peroxidative damage has been introduced, involving the probe BODIPY (581/591) C11, which readily incorporates into cells and undergoes a spectral emission shift when attacked by reactive oxygen metabolites. We have examined the applicability of this probe as an indicator of oxidative stress in human sperm populations using flow cytometry as an end point. The measurement of peroxidation with BODIPY C11 demonstrated significant dependence on the presence of a ferrous ion promoter (P < 0.001), which was significantly enhanced in sperm recovered from low-density Percoll fractions (P < 0.05) and was particularly damaging to the sperm midpiece. Iron-induced radical formation was suppressed by ascorbate in a dose-dependent manner (P < 0.001) and could only be promoted by Fe(II) and Cu(II); nickel, zinc and Fe(III) were ineffective. The Fe(II)-promoted BODIPY C11 signal was significantly correlated with the measurement of reactive oxygen species generation with dihydroethidium. We conclude that BODIPY C11 is an extremely useful probe for indexing peroxidative damage in human spermatozoa.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalMolecular Human Reproduction
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 2007
Externally publishedYes

Fingerprint

Lipid Peroxidation
Spermatozoa
Sperm Midpiece
Biochemical Phenomena
Nickel
Infertility
Zinc
Reactive Oxygen Species
Flow Cytometry
Oxidative Stress
Iron
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Ions
Oxygen
Population

Cite this

Aitken, R. John ; Wingate, Jordana K. ; De Iuliis, Geoffry N. ; McLaughlin, Eileen A. / Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. In: Molecular Human Reproduction. 2007 ; Vol. 13, No. 4. pp. 203-211.
@article{4ebf34ef0e7b4f2a94d434be38de68a2,
title = "Analysis of lipid peroxidation in human spermatozoa using BODIPY C11",
abstract = "Lipid peroxidation is known to be a major factor in the aetiology of defective sperm function. Although biochemical assays for this process exist, they are relatively insensitive and require large numbers of spermatozoa; a condition that cannot be met with many infertility specimens. Recently, a new approach for monitoring peroxidative damage has been introduced, involving the probe BODIPY (581/591) C11, which readily incorporates into cells and undergoes a spectral emission shift when attacked by reactive oxygen metabolites. We have examined the applicability of this probe as an indicator of oxidative stress in human sperm populations using flow cytometry as an end point. The measurement of peroxidation with BODIPY C11 demonstrated significant dependence on the presence of a ferrous ion promoter (P < 0.001), which was significantly enhanced in sperm recovered from low-density Percoll fractions (P < 0.05) and was particularly damaging to the sperm midpiece. Iron-induced radical formation was suppressed by ascorbate in a dose-dependent manner (P < 0.001) and could only be promoted by Fe(II) and Cu(II); nickel, zinc and Fe(III) were ineffective. The Fe(II)-promoted BODIPY C11 signal was significantly correlated with the measurement of reactive oxygen species generation with dihydroethidium. We conclude that BODIPY C11 is an extremely useful probe for indexing peroxidative damage in human spermatozoa.",
keywords = "BODIPY C, Human spermatozoa, Lipid peroxidation, Oxidative stress",
author = "Aitken, {R. John} and Wingate, {Jordana K.} and {De Iuliis}, {Geoffry N.} and McLaughlin, {Eileen A.}",
year = "2007",
month = "4",
doi = "10.1093/molehr/gal119",
language = "English",
volume = "13",
pages = "203--211",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "4",

}

Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. / Aitken, R. John; Wingate, Jordana K.; De Iuliis, Geoffry N.; McLaughlin, Eileen A.

In: Molecular Human Reproduction, Vol. 13, No. 4, 04.2007, p. 203-211.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of lipid peroxidation in human spermatozoa using BODIPY C11

AU - Aitken, R. John

AU - Wingate, Jordana K.

AU - De Iuliis, Geoffry N.

AU - McLaughlin, Eileen A.

PY - 2007/4

Y1 - 2007/4

N2 - Lipid peroxidation is known to be a major factor in the aetiology of defective sperm function. Although biochemical assays for this process exist, they are relatively insensitive and require large numbers of spermatozoa; a condition that cannot be met with many infertility specimens. Recently, a new approach for monitoring peroxidative damage has been introduced, involving the probe BODIPY (581/591) C11, which readily incorporates into cells and undergoes a spectral emission shift when attacked by reactive oxygen metabolites. We have examined the applicability of this probe as an indicator of oxidative stress in human sperm populations using flow cytometry as an end point. The measurement of peroxidation with BODIPY C11 demonstrated significant dependence on the presence of a ferrous ion promoter (P < 0.001), which was significantly enhanced in sperm recovered from low-density Percoll fractions (P < 0.05) and was particularly damaging to the sperm midpiece. Iron-induced radical formation was suppressed by ascorbate in a dose-dependent manner (P < 0.001) and could only be promoted by Fe(II) and Cu(II); nickel, zinc and Fe(III) were ineffective. The Fe(II)-promoted BODIPY C11 signal was significantly correlated with the measurement of reactive oxygen species generation with dihydroethidium. We conclude that BODIPY C11 is an extremely useful probe for indexing peroxidative damage in human spermatozoa.

AB - Lipid peroxidation is known to be a major factor in the aetiology of defective sperm function. Although biochemical assays for this process exist, they are relatively insensitive and require large numbers of spermatozoa; a condition that cannot be met with many infertility specimens. Recently, a new approach for monitoring peroxidative damage has been introduced, involving the probe BODIPY (581/591) C11, which readily incorporates into cells and undergoes a spectral emission shift when attacked by reactive oxygen metabolites. We have examined the applicability of this probe as an indicator of oxidative stress in human sperm populations using flow cytometry as an end point. The measurement of peroxidation with BODIPY C11 demonstrated significant dependence on the presence of a ferrous ion promoter (P < 0.001), which was significantly enhanced in sperm recovered from low-density Percoll fractions (P < 0.05) and was particularly damaging to the sperm midpiece. Iron-induced radical formation was suppressed by ascorbate in a dose-dependent manner (P < 0.001) and could only be promoted by Fe(II) and Cu(II); nickel, zinc and Fe(III) were ineffective. The Fe(II)-promoted BODIPY C11 signal was significantly correlated with the measurement of reactive oxygen species generation with dihydroethidium. We conclude that BODIPY C11 is an extremely useful probe for indexing peroxidative damage in human spermatozoa.

KW - BODIPY C

KW - Human spermatozoa

KW - Lipid peroxidation

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=34147121187&partnerID=8YFLogxK

U2 - 10.1093/molehr/gal119

DO - 10.1093/molehr/gal119

M3 - Article

VL - 13

SP - 203

EP - 211

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 4

ER -