Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus

Simon Harvey, Chuta Boomphakdee, R. Campos-Ramos, Tariq Ezaz, D. K. Griffin, N. R. Bromage, P. Penman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In the Nile tilapia, Oreochromis niloticus, sex determination is primarily genetic, with XX females and XY males. While the X and Y chromosomes (the largest pair) cannot be distinguished in mitotic chromosome spreads, analysis of comparative hybridization of X and Y chromosome derived probes (produced, by microdissection and DOP-PCR, from XX and YY genotypes, respectively) to different genotypes (XX, XY and YY) has demonstrated that sequence differences exist between the sex chromosomes. Here we report the characterization of these probes, showing that a significant proportion of the amplified sequences represent various transposable elements. We further demonstrate that concentrations of a number of these individual elements are found on the sex chromosomes and that the distribution of two such elements differs between the X and Y chromosomes. These findings are discussed in relation to sex chromosome differentiation in O. niloticus and to the changes expected during the early stages of sex chromosome evolution
Original languageEnglish
Pages (from-to)314-319
Number of pages6
JournalCytogenetic and Genome Research
Volume101
Issue number3-4
DOIs
Publication statusPublished - 2003
Externally publishedYes

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Cichlids
Sex Chromosomes
Nucleic Acid Repetitive Sequences
Y Chromosome
X Chromosome
DNA
Genotype
Microdissection
Sex Differentiation
Sex Distribution
DNA Transposable Elements
Chromosomes
Polymerase Chain Reaction

Cite this

Harvey, S., Boomphakdee, C., Campos-Ramos, R., Ezaz, T., Griffin, D. K., Bromage, N. R., & Penman, P. (2003). Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. Cytogenetic and Genome Research, 101(3-4), 314-319. https://doi.org/10.1159/000074355
Harvey, Simon ; Boomphakdee, Chuta ; Campos-Ramos, R. ; Ezaz, Tariq ; Griffin, D. K. ; Bromage, N. R. ; Penman, P. / Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. In: Cytogenetic and Genome Research. 2003 ; Vol. 101, No. 3-4. pp. 314-319.
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Harvey, S, Boomphakdee, C, Campos-Ramos, R, Ezaz, T, Griffin, DK, Bromage, NR & Penman, P 2003, 'Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus', Cytogenetic and Genome Research, vol. 101, no. 3-4, pp. 314-319. https://doi.org/10.1159/000074355

Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. / Harvey, Simon; Boomphakdee, Chuta; Campos-Ramos, R.; Ezaz, Tariq; Griffin, D. K.; Bromage, N. R.; Penman, P.

In: Cytogenetic and Genome Research, Vol. 101, No. 3-4, 2003, p. 314-319.

Research output: Contribution to journalArticle

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AU - Boomphakdee, Chuta

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AB - In the Nile tilapia, Oreochromis niloticus, sex determination is primarily genetic, with XX females and XY males. While the X and Y chromosomes (the largest pair) cannot be distinguished in mitotic chromosome spreads, analysis of comparative hybridization of X and Y chromosome derived probes (produced, by microdissection and DOP-PCR, from XX and YY genotypes, respectively) to different genotypes (XX, XY and YY) has demonstrated that sequence differences exist between the sex chromosomes. Here we report the characterization of these probes, showing that a significant proportion of the amplified sequences represent various transposable elements. We further demonstrate that concentrations of a number of these individual elements are found on the sex chromosomes and that the distribution of two such elements differs between the X and Y chromosomes. These findings are discussed in relation to sex chromosome differentiation in O. niloticus and to the changes expected during the early stages of sex chromosome evolution

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