Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo

Christian J. Cook, Carrick E. Devine

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.

Original languageEnglish
Pages (from-to)1108-1111
Number of pages4
JournalElectroanalysis
Volume10
Issue number16
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Antibodies
Neurotransmitter Agents
Hormones
Electrodes
Animals
Immunosensors
Oxytocin
Antigens
Time measurement
Prolactin
Brain
Blood
Tissue
Sampling
Monitoring
Sensors
Microdialysis

Cite this

Cook, Christian J. ; Devine, Carrick E. / Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo. In: Electroanalysis. 1998 ; Vol. 10, No. 16. pp. 1108-1111.
@article{0a7e89f58e3242d391256d3e8817ea3b,
title = "Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo",
abstract = "Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.",
keywords = "Antibody based electrodes, Hormonal and neurotransmitter measurements, Oxytocin, Prolactin",
author = "Cook, {Christian J.} and Devine, {Carrick E.}",
year = "1998",
doi = "10.1002/(SICI)1521-4109(199811)10:16<1108::AID-ELAN1108>3.0.CO;2-1",
language = "English",
volume = "10",
pages = "1108--1111",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "16",

}

Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo. / Cook, Christian J.; Devine, Carrick E.

In: Electroanalysis, Vol. 10, No. 16, 1998, p. 1108-1111.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo

AU - Cook, Christian J.

AU - Devine, Carrick E.

PY - 1998

Y1 - 1998

N2 - Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.

AB - Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.

KW - Antibody based electrodes

KW - Hormonal and neurotransmitter measurements

KW - Oxytocin

KW - Prolactin

UR - http://www.scopus.com/inward/record.url?scp=0003907128&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1521-4109(199811)10:16<1108::AID-ELAN1108>3.0.CO;2-1

DO - 10.1002/(SICI)1521-4109(199811)10:16<1108::AID-ELAN1108>3.0.CO;2-1

M3 - Article

VL - 10

SP - 1108

EP - 1111

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 16

ER -