Antibody-Based Electrodes for Hormonal and Neurotransmitter Measurements in Vivo

Christian J. Cook, Carrick E. Devine

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.

Original languageEnglish
Pages (from-to)1108-1111
Number of pages4
Issue number16
Publication statusPublished - 1998
Externally publishedYes


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