Immunosensors, in which the interaction between an antigen of interest and an antibody can be used for detection purposes, have found multiple uses in biological sciences. Microdialysis techniques provide a format for sampling, with low invasiveness, from circulation and tissues of conscious animals. We have combined these two designs to construct antibody-based electrodes housed within microdialysis probes that allow 'relative' real-time measurements of several hormones and putative neurotransmitters in vivo. Measurements of two hormones, prolactin and oxytocin, were made from areas of the brain, by using a combination imummunosensor microdialysis techniques, in behaving sheep. Sensors were viable for between 200 and 400 sequential measures and for at least 6 hours in vivo. Detection sensitivity was between I and 1 pg/mL. The results suggest the possibility of monitoring these nuerohumoral factors within the blood, or organ systems, of conscious behaving animals.
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|Published - 1998