Arsenic species determination in biological tissues by HPLC-ICP-MS and HPLC-HG-ICP-MS

Jason Kirby, Bill Maher, Michael Ellwood, Frank Krikowa

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    Abstract

    The use of high-pressure liquid chromatography coupled directly or by a hydride generation system to an inductively coupled plasma mass spectrometer for the unambiguous measurement of 13 arsenic species in marine biological extracts is described. The use of two chromatography systems; a Supelcosil LC-SCX cation-exchange column eluted with a 20 mM pyridine mobile phase adjusted to pH 2.2 and 2.6 with formic acid, with a flow rate of 1.5 mL min-1 at 40°C, and a Hamilton PRP-X100 anion-exchange column eluted with 20 mM NH4H2PO4 buffer at pH 5.6, with a flow rate of 1.5 mL min-1 at 40°C, was required to separate and quantify cation and anion arsenic species. Under these conditions, arsenous acid could not be separated from other arsenic species and required the use of an additional hydride generation step. Arsenic species concentrations in a locally available Tasmanian kelp (Durvillea potatorum), a certified reference material (DORM-2), and a range of commercially available macroalgae supplements and sushi seaweeds have been measured and are provided for use as in-house quality control samples to assess the effectiveness of sample preparation, extraction, and measurement techniques
    Original languageEnglish
    Pages (from-to)957-966
    Number of pages10
    JournalAustralian Journal of Chemistry: an international journal for chemical science
    Volume57
    Issue number10
    DOIs
    Publication statusPublished - 2004

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    Arsenic
    Tissue
    formic acid
    Hydrides
    Anions
    Cations
    Flow rate
    High pressure liquid chromatography
    Seaweed
    Inductively coupled plasma
    Mass spectrometers
    Chromatography
    Quality control
    Buffers

    Cite this

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    title = "Arsenic species determination in biological tissues by HPLC-ICP-MS and HPLC-HG-ICP-MS",
    abstract = "The use of high-pressure liquid chromatography coupled directly or by a hydride generation system to an inductively coupled plasma mass spectrometer for the unambiguous measurement of 13 arsenic species in marine biological extracts is described. The use of two chromatography systems; a Supelcosil LC-SCX cation-exchange column eluted with a 20 mM pyridine mobile phase adjusted to pH 2.2 and 2.6 with formic acid, with a flow rate of 1.5 mL min-1 at 40°C, and a Hamilton PRP-X100 anion-exchange column eluted with 20 mM NH4H2PO4 buffer at pH 5.6, with a flow rate of 1.5 mL min-1 at 40°C, was required to separate and quantify cation and anion arsenic species. Under these conditions, arsenous acid could not be separated from other arsenic species and required the use of an additional hydride generation step. Arsenic species concentrations in a locally available Tasmanian kelp (Durvillea potatorum), a certified reference material (DORM-2), and a range of commercially available macroalgae supplements and sushi seaweeds have been measured and are provided for use as in-house quality control samples to assess the effectiveness of sample preparation, extraction, and measurement techniques",
    author = "Jason Kirby and Bill Maher and Michael Ellwood and Frank Krikowa",
    year = "2004",
    doi = "10.1071/CH04094",
    language = "English",
    volume = "57",
    pages = "957--966",
    journal = "Australian Journal of Chemistry",
    issn = "0004-9425",
    publisher = "CSIRO",
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    TY - JOUR

    T1 - Arsenic species determination in biological tissues by HPLC-ICP-MS and HPLC-HG-ICP-MS

    AU - Kirby, Jason

    AU - Maher, Bill

    AU - Ellwood, Michael

    AU - Krikowa, Frank

    PY - 2004

    Y1 - 2004

    N2 - The use of high-pressure liquid chromatography coupled directly or by a hydride generation system to an inductively coupled plasma mass spectrometer for the unambiguous measurement of 13 arsenic species in marine biological extracts is described. The use of two chromatography systems; a Supelcosil LC-SCX cation-exchange column eluted with a 20 mM pyridine mobile phase adjusted to pH 2.2 and 2.6 with formic acid, with a flow rate of 1.5 mL min-1 at 40°C, and a Hamilton PRP-X100 anion-exchange column eluted with 20 mM NH4H2PO4 buffer at pH 5.6, with a flow rate of 1.5 mL min-1 at 40°C, was required to separate and quantify cation and anion arsenic species. Under these conditions, arsenous acid could not be separated from other arsenic species and required the use of an additional hydride generation step. Arsenic species concentrations in a locally available Tasmanian kelp (Durvillea potatorum), a certified reference material (DORM-2), and a range of commercially available macroalgae supplements and sushi seaweeds have been measured and are provided for use as in-house quality control samples to assess the effectiveness of sample preparation, extraction, and measurement techniques

    AB - The use of high-pressure liquid chromatography coupled directly or by a hydride generation system to an inductively coupled plasma mass spectrometer for the unambiguous measurement of 13 arsenic species in marine biological extracts is described. The use of two chromatography systems; a Supelcosil LC-SCX cation-exchange column eluted with a 20 mM pyridine mobile phase adjusted to pH 2.2 and 2.6 with formic acid, with a flow rate of 1.5 mL min-1 at 40°C, and a Hamilton PRP-X100 anion-exchange column eluted with 20 mM NH4H2PO4 buffer at pH 5.6, with a flow rate of 1.5 mL min-1 at 40°C, was required to separate and quantify cation and anion arsenic species. Under these conditions, arsenous acid could not be separated from other arsenic species and required the use of an additional hydride generation step. Arsenic species concentrations in a locally available Tasmanian kelp (Durvillea potatorum), a certified reference material (DORM-2), and a range of commercially available macroalgae supplements and sushi seaweeds have been measured and are provided for use as in-house quality control samples to assess the effectiveness of sample preparation, extraction, and measurement techniques

    U2 - 10.1071/CH04094

    DO - 10.1071/CH04094

    M3 - Article

    VL - 57

    SP - 957

    EP - 966

    JO - Australian Journal of Chemistry

    JF - Australian Journal of Chemistry

    SN - 0004-9425

    IS - 10

    ER -