Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments

Ronald Glabonjat, Josef Ehgartner, Elliott Duncan, Georg Rabner, Kenneth Jensen, Frank Krikowa, Bill MAHER, Kevin Francesconi

Research output: Contribution to journalArticle

Abstract

The influence of arsenate and phosphate levels in water on the formation of arsenic-containing lipids (arsenolipids) and water-soluble arsenicals by a unicellular marine alga was investigated by exposing Dunaliella tertiolecta to five regimes of arsenic and phosphate, and determining the biosynthesized organoarsenicals with HPLC/mass spectrometry. Under all conditions, the major arsenolipid produced by D. tertiolecta was the novel phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside (AsSugPhytol546) representing ca. 35–65% of total arsenolipids. The new compound contains a phytol aglycone and a methoxy group replacing a sugar hydroxyl – two structural features not previously observed for arsenolipids. Minor arsenolipids were several previously reported arsenosugar phospholipids (AsSugPLs, in particular AsSugPL958 and the previously unknown AsSugPL978), the relative quantities of which increased with increasing phosphate exposure, and an arsenic-containing hydrocarbon (AsHC360), which remained unaffected by the different treatments. The relative amount of total arsenolipids produced by D. tertiolecta remained remarkably constant (ca. 45% of total As) and independent of the culture conditions. In contrast, with rising As-concentrations we observed an increase of hydrophilic arsenicals, which were dominated by arsenate and arsenosugars. The results highlight a possible major difference in arsenic biochemistry between macroalgae and unicellular algae with potential implications for how various algae handle their natural arsenic exposure in the world's oceans.
LanguageEnglish
Pages145-153
Number of pages9
JournalMetallomics
Volume10
Issue number1
DOIs
StatePublished - 2018

Fingerprint

Biosynthesis
Arsenic
Algae
Lipids
Experiments
Arsenicals
Phosphates
Phytol
Seaweed
Biochemistry
Water
Phospholipids
Hydrocarbons
Oceans and Seas
Sugars
Hydroxyl Radical
Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography

Cite this

Glabonjat, R., Ehgartner, J., Duncan, E., Rabner, G., Jensen, K., Krikowa, F., ... Francesconi, K. (2018). Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments. Metallomics, 10(1), 145-153. DOI: 10.1039/C7MT00249A
Glabonjat, Ronald ; Ehgartner, Josef ; Duncan, Elliott ; Rabner, Georg ; Jensen, Kenneth ; Krikowa, Frank ; MAHER, Bill ; Francesconi, Kevin. / Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments. In: Metallomics. 2018 ; Vol. 10, No. 1. pp. 145-153
@article{651cf22215a740ecaec177447d5dd885,
title = "Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments",
abstract = "The influence of arsenate and phosphate levels in water on the formation of arsenic-containing lipids (arsenolipids) and water-soluble arsenicals by a unicellular marine alga was investigated by exposing Dunaliella tertiolecta to five regimes of arsenic and phosphate, and determining the biosynthesized organoarsenicals with HPLC/mass spectrometry. Under all conditions, the major arsenolipid produced by D. tertiolecta was the novel phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside (AsSugPhytol546) representing ca. 35–65{\%} of total arsenolipids. The new compound contains a phytol aglycone and a methoxy group replacing a sugar hydroxyl – two structural features not previously observed for arsenolipids. Minor arsenolipids were several previously reported arsenosugar phospholipids (AsSugPLs, in particular AsSugPL958 and the previously unknown AsSugPL978), the relative quantities of which increased with increasing phosphate exposure, and an arsenic-containing hydrocarbon (AsHC360), which remained unaffected by the different treatments. The relative amount of total arsenolipids produced by D. tertiolecta remained remarkably constant (ca. 45{\%} of total As) and independent of the culture conditions. In contrast, with rising As-concentrations we observed an increase of hydrophilic arsenicals, which were dominated by arsenate and arsenosugars. The results highlight a possible major difference in arsenic biochemistry between macroalgae and unicellular algae with potential implications for how various algae handle their natural arsenic exposure in the world's oceans.",
author = "Ronald Glabonjat and Josef Ehgartner and Elliott Duncan and Georg Rabner and Kenneth Jensen and Frank Krikowa and Bill MAHER and Kevin Francesconi",
year = "2018",
doi = "10.1039/C7MT00249A",
language = "English",
volume = "10",
pages = "145--153",
journal = "Metallomics",
issn = "1756-5901",
publisher = "Royal Society of Chemistry",
number = "1",

}

Glabonjat, R, Ehgartner, J, Duncan, E, Rabner, G, Jensen, K, Krikowa, F, MAHER, B & Francesconi, K 2018, 'Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments' Metallomics, vol. 10, no. 1, pp. 145-153. DOI: 10.1039/C7MT00249A

Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments. / Glabonjat, Ronald; Ehgartner, Josef; Duncan, Elliott; Rabner, Georg; Jensen, Kenneth; Krikowa, Frank; MAHER, Bill; Francesconi, Kevin.

In: Metallomics, Vol. 10, No. 1, 2018, p. 145-153.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Arsenolipid biosynthesis by the unicellular alga Dunaliella tertiolecta is influenced by As/P ratio in culture experiments

AU - Glabonjat,Ronald

AU - Ehgartner,Josef

AU - Duncan,Elliott

AU - Rabner,Georg

AU - Jensen,Kenneth

AU - Krikowa,Frank

AU - MAHER,Bill

AU - Francesconi,Kevin

PY - 2018

Y1 - 2018

N2 - The influence of arsenate and phosphate levels in water on the formation of arsenic-containing lipids (arsenolipids) and water-soluble arsenicals by a unicellular marine alga was investigated by exposing Dunaliella tertiolecta to five regimes of arsenic and phosphate, and determining the biosynthesized organoarsenicals with HPLC/mass spectrometry. Under all conditions, the major arsenolipid produced by D. tertiolecta was the novel phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside (AsSugPhytol546) representing ca. 35–65% of total arsenolipids. The new compound contains a phytol aglycone and a methoxy group replacing a sugar hydroxyl – two structural features not previously observed for arsenolipids. Minor arsenolipids were several previously reported arsenosugar phospholipids (AsSugPLs, in particular AsSugPL958 and the previously unknown AsSugPL978), the relative quantities of which increased with increasing phosphate exposure, and an arsenic-containing hydrocarbon (AsHC360), which remained unaffected by the different treatments. The relative amount of total arsenolipids produced by D. tertiolecta remained remarkably constant (ca. 45% of total As) and independent of the culture conditions. In contrast, with rising As-concentrations we observed an increase of hydrophilic arsenicals, which were dominated by arsenate and arsenosugars. The results highlight a possible major difference in arsenic biochemistry between macroalgae and unicellular algae with potential implications for how various algae handle their natural arsenic exposure in the world's oceans.

AB - The influence of arsenate and phosphate levels in water on the formation of arsenic-containing lipids (arsenolipids) and water-soluble arsenicals by a unicellular marine alga was investigated by exposing Dunaliella tertiolecta to five regimes of arsenic and phosphate, and determining the biosynthesized organoarsenicals with HPLC/mass spectrometry. Under all conditions, the major arsenolipid produced by D. tertiolecta was the novel phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside (AsSugPhytol546) representing ca. 35–65% of total arsenolipids. The new compound contains a phytol aglycone and a methoxy group replacing a sugar hydroxyl – two structural features not previously observed for arsenolipids. Minor arsenolipids were several previously reported arsenosugar phospholipids (AsSugPLs, in particular AsSugPL958 and the previously unknown AsSugPL978), the relative quantities of which increased with increasing phosphate exposure, and an arsenic-containing hydrocarbon (AsHC360), which remained unaffected by the different treatments. The relative amount of total arsenolipids produced by D. tertiolecta remained remarkably constant (ca. 45% of total As) and independent of the culture conditions. In contrast, with rising As-concentrations we observed an increase of hydrophilic arsenicals, which were dominated by arsenate and arsenosugars. The results highlight a possible major difference in arsenic biochemistry between macroalgae and unicellular algae with potential implications for how various algae handle their natural arsenic exposure in the world's oceans.

U2 - 10.1039/C7MT00249A

DO - 10.1039/C7MT00249A

M3 - Article

VL - 10

SP - 145

EP - 153

JO - Metallomics

T2 - Metallomics

JF - Metallomics

SN - 1756-5901

IS - 1

ER -