Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position

S M Heffernan, Liam P Kilduff, R M Erskine, S H Day, J S McPhee, G E McMahon, G K Stebbings, J P H Neale, S J Lockey, W J Ribbans, C J Cook, B Vance, S M Raleigh, C Roberts, M A Bennett, Guoxiu Wang, M Collins, Y P Pitsiladis, A G Williams

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Abstract

We aimed to quantify the ACE I/D and ACTN3 R577X (rs1815739) genetic variants in elite rugby athletes (rugby union and league) and compare genotype frequencies to controls and between playing positions. The rugby athlete cohort consisted of 507 Caucasian men, including 431 rugby union athletes that for some analyses were divided into backs and forwards and into specific positional groups: front five, back row, half backs, centers, and back three. Controls were 710 Caucasian men and women. Real-time PCR of genomic DNA was used to determine genotypes using TaqMan probes and groups were compared using χ(2) and odds ratio (OR) statistics. Correction of P values for multiple comparisons was according to Benjamini-Hochberg. There was no difference in ACE I/D genotype between groups. ACTN3 XX genotype tended to be underrepresented in rugby union backs (15.7%) compared with forwards (24.8%, P = 0.06). Interestingly, the 69 back three players (wings and full backs) in rugby union included only six XX genotype individuals (8.7%), with the R allele more common in the back three (68.8%) than controls (58.0%; χ(2) = 6.672, P = 0.04; OR = 1.60) and forwards (47.5%; χ(2) = 11.768, P = 0.01; OR = 2.00). Association of ACTN3 R577X with playing position in elite rugby union athletes suggests inherited fatigue resistance is more prevalent in forwards, while inherited sprint ability is more prevalent in backs, especially wings and full backs. These results also demonstrate the advantage of focusing genetic studies on a large cohort within a single sport, especially when intrasport positional differences exist, instead of combining several sports with varied demands and athlete characteristics.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalPhysiological Genomics
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2016
Externally publishedYes

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Football
Athletes
Genotype
Genes
Odds Ratio
Sports
Fatigue
Real-Time Polymerase Chain Reaction
Alleles
DNA

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Heffernan, S. M., Kilduff, L. P., Erskine, R. M., Day, S. H., McPhee, J. S., McMahon, G. E., ... Williams, A. G. (2016). Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position. Physiological Genomics, 48(3), 196-201. https://doi.org/10.1152/physiolgenomics.00107.2015
Heffernan, S M ; Kilduff, Liam P ; Erskine, R M ; Day, S H ; McPhee, J S ; McMahon, G E ; Stebbings, G K ; Neale, J P H ; Lockey, S J ; Ribbans, W J ; Cook, C J ; Vance, B ; Raleigh, S M ; Roberts, C ; Bennett, M A ; Wang, Guoxiu ; Collins, M ; Pitsiladis, Y P ; Williams, A G. / Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position. In: Physiological Genomics. 2016 ; Vol. 48, No. 3. pp. 196-201.
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abstract = "We aimed to quantify the ACE I/D and ACTN3 R577X (rs1815739) genetic variants in elite rugby athletes (rugby union and league) and compare genotype frequencies to controls and between playing positions. The rugby athlete cohort consisted of 507 Caucasian men, including 431 rugby union athletes that for some analyses were divided into backs and forwards and into specific positional groups: front five, back row, half backs, centers, and back three. Controls were 710 Caucasian men and women. Real-time PCR of genomic DNA was used to determine genotypes using TaqMan probes and groups were compared using χ(2) and odds ratio (OR) statistics. Correction of P values for multiple comparisons was according to Benjamini-Hochberg. There was no difference in ACE I/D genotype between groups. ACTN3 XX genotype tended to be underrepresented in rugby union backs (15.7{\%}) compared with forwards (24.8{\%}, P = 0.06). Interestingly, the 69 back three players (wings and full backs) in rugby union included only six XX genotype individuals (8.7{\%}), with the R allele more common in the back three (68.8{\%}) than controls (58.0{\%}; χ(2) = 6.672, P = 0.04; OR = 1.60) and forwards (47.5{\%}; χ(2) = 11.768, P = 0.01; OR = 2.00). Association of ACTN3 R577X with playing position in elite rugby union athletes suggests inherited fatigue resistance is more prevalent in forwards, while inherited sprint ability is more prevalent in backs, especially wings and full backs. These results also demonstrate the advantage of focusing genetic studies on a large cohort within a single sport, especially when intrasport positional differences exist, instead of combining several sports with varied demands and athlete characteristics.",
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Heffernan, SM, Kilduff, LP, Erskine, RM, Day, SH, McPhee, JS, McMahon, GE, Stebbings, GK, Neale, JPH, Lockey, SJ, Ribbans, WJ, Cook, CJ, Vance, B, Raleigh, SM, Roberts, C, Bennett, MA, Wang, G, Collins, M, Pitsiladis, YP & Williams, AG 2016, 'Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position', Physiological Genomics, vol. 48, no. 3, pp. 196-201. https://doi.org/10.1152/physiolgenomics.00107.2015

Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position. / Heffernan, S M; Kilduff, Liam P; Erskine, R M; Day, S H; McPhee, J S; McMahon, G E; Stebbings, G K; Neale, J P H; Lockey, S J; Ribbans, W J; Cook, C J; Vance, B; Raleigh, S M; Roberts, C; Bennett, M A; Wang, Guoxiu; Collins, M; Pitsiladis, Y P; Williams, A G.

In: Physiological Genomics, Vol. 48, No. 3, 03.2016, p. 196-201.

Research output: Contribution to journalArticle

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AU - Kilduff, Liam P

AU - Erskine, R M

AU - Day, S H

AU - McPhee, J S

AU - McMahon, G E

AU - Stebbings, G K

AU - Neale, J P H

AU - Lockey, S J

AU - Ribbans, W J

AU - Cook, C J

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