Association of matrix protein of respiratory syncytial virus with the host cell membrane of infected cells

A Marty, J Meanger, J Mills, B Shields, R Ghildyal

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The matrix protein of paramyxoviruses plays an important role in virus assembly through its interactions with cell membrane, virus envelope and virus nucleocapsid. In the present study, we investigated the possible association of respiratory syncytial virus (RSV) matrix (M) protein with the plasma membrane of infected cells. Using confocal microscopy we found that M was present at the cytoplasmic side of the plasma membrane. We used flotation gradients to purify membranes from RSV infected cells and treated them with cold Triton X-100 to obtain lipid rafts in the insoluble fraction. Western blot of the lipid raft fraction with specific antibodies showed that it contained M, as well as G (attachment) and N (nucleocapsid) proteins. We also found that RSV purified on sucrose gradients contained lipid raft markers. Together, our data suggest that RSV uses lipid rafts for assembly and budding.

Original languageEnglish
Pages (from-to)199-210
Number of pages12
JournalArchives of Virology
Volume149
Issue number1
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Respiratory Syncytial Viruses
Cell Membrane
Lipids
Proteins
Viruses
Nucleocapsid Proteins
Virus Assembly
Nucleocapsid
Octoxynol
Confocal Microscopy
Sucrose
Western Blotting
Membranes
Antibodies

Cite this

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title = "Association of matrix protein of respiratory syncytial virus with the host cell membrane of infected cells",
abstract = "The matrix protein of paramyxoviruses plays an important role in virus assembly through its interactions with cell membrane, virus envelope and virus nucleocapsid. In the present study, we investigated the possible association of respiratory syncytial virus (RSV) matrix (M) protein with the plasma membrane of infected cells. Using confocal microscopy we found that M was present at the cytoplasmic side of the plasma membrane. We used flotation gradients to purify membranes from RSV infected cells and treated them with cold Triton X-100 to obtain lipid rafts in the insoluble fraction. Western blot of the lipid raft fraction with specific antibodies showed that it contained M, as well as G (attachment) and N (nucleocapsid) proteins. We also found that RSV purified on sucrose gradients contained lipid raft markers. Together, our data suggest that RSV uses lipid rafts for assembly and budding.",
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Association of matrix protein of respiratory syncytial virus with the host cell membrane of infected cells. / Marty, A; Meanger, J; Mills, J; Shields, B; Ghildyal, R.

In: Archives of Virology, Vol. 149, No. 1, 2004, p. 199-210.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of matrix protein of respiratory syncytial virus with the host cell membrane of infected cells

AU - Marty, A

AU - Meanger, J

AU - Mills, J

AU - Shields, B

AU - Ghildyal, R

PY - 2004

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AB - The matrix protein of paramyxoviruses plays an important role in virus assembly through its interactions with cell membrane, virus envelope and virus nucleocapsid. In the present study, we investigated the possible association of respiratory syncytial virus (RSV) matrix (M) protein with the plasma membrane of infected cells. Using confocal microscopy we found that M was present at the cytoplasmic side of the plasma membrane. We used flotation gradients to purify membranes from RSV infected cells and treated them with cold Triton X-100 to obtain lipid rafts in the insoluble fraction. Western blot of the lipid raft fraction with specific antibodies showed that it contained M, as well as G (attachment) and N (nucleocapsid) proteins. We also found that RSV purified on sucrose gradients contained lipid raft markers. Together, our data suggest that RSV uses lipid rafts for assembly and budding.

KW - Cell Line

KW - Cell Membrane

KW - Humans

KW - Membrane Microdomains

KW - Respiratory Syncytial Viruses

KW - Viral Matrix Proteins

KW - Viral Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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DO - 10.1007/s00705-003-0183-9

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