Australian marsupial species identification

Linzi Wilson-Wilde, Janette Norman, James Robertson, Stephen Sarre, Arthur Georges

Research output: A Conference proceeding or a Chapter in BookConference contribution

1 Citation (Scopus)

Abstract

Wildlife crime, the illegal trade in animals and animal products, is a growing concern and valued at up to US$20 billion globally per year. Australia is often targeted for its unique fauna, proximity to South East Asia and porous borders. Marsupials of the order Diprotodontia (including koala, wombats, possums, gliders, kangaroos) are sometimes targeted for their skin, meat and for the pet trade. However, species identification for forensic purposes must be underpinned by robust phylogenetic information. A Diprotodont phylogeny containing a large number of taxa generated from nuclear and mitochondrial data has not yet been constructed. Here the mitochondrial (COI and ND2) and nuclear markers (APOB, IRBP and GAPD) are combined to create a more robust phylogeny to underpin a species identification method for the marsupial order Diprotodontia. Mitochondrial markers were combined with nuclear markers to amplify 27 genera of Diprotodontia. Data was analysed using a likelihood method. The combined data set resolved two suborders: Vombatiformes and Phalangeriformes. Phalangeriformes was subsequently split into two clades. The first clade contained the Macropodiformes and Burramyidae. The second clade contained Petauridae, grouping with Phalangeroidea. Of the markers tested, ND2 provided the greatest level of diagnostic accuracy and could be used as a forensic species identification tool for Diprotodonts, with appropriate validation.
Original languageEnglish
Title of host publicationForensic Science International
Subtitle of host publicationGenetics Supplement Series
Place of PublicationNetherlands
PublisherElsevier
Pages543-544
Number of pages2
Volume3
DOIs
Publication statusPublished - 2011
EventWorld Congress of the International Society for Forensic Genetics - University of Vienna, Vienna, Austria
Duration: 29 Aug 20113 Sep 2011
https://www.isfg.org/files/ISFG2011_program.pdf (Conference Program)

Publication series

NameGenetics supplement series
PublisherElsevier Science
Number1
Volume3
ISSN (Print)1875-175X

Conference

ConferenceWorld Congress of the International Society for Forensic Genetics
Abbreviated titleISFG
CountryAustria
CityVienna
Period29/08/113/09/11
Internet address

Fingerprint

Metatheria
phylogeny
crime
Macropodidae
animal products
skin (animal)
pets
wildlife
meat
fauna
Diprotodontia
species identification
animals
forensic sciences
methodology

Cite this

Wilson-Wilde, L., Norman, J., Robertson, J., Sarre, S., & Georges, A. (2011). Australian marsupial species identification. In Forensic Science International: Genetics Supplement Series (Vol. 3, pp. 543-544). (Genetics supplement series; Vol. 3, No. 1). Netherlands: Elsevier. https://doi.org/10.1016/j.fsigss.2011.10.014
Wilson-Wilde, Linzi ; Norman, Janette ; Robertson, James ; Sarre, Stephen ; Georges, Arthur. / Australian marsupial species identification. Forensic Science International: Genetics Supplement Series. Vol. 3 Netherlands : Elsevier, 2011. pp. 543-544 (Genetics supplement series; 1).
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Wilson-Wilde, L, Norman, J, Robertson, J, Sarre, S & Georges, A 2011, Australian marsupial species identification. in Forensic Science International: Genetics Supplement Series. vol. 3, Genetics supplement series, no. 1, vol. 3, Elsevier, Netherlands, pp. 543-544, World Congress of the International Society for Forensic Genetics, Vienna, Austria, 29/08/11. https://doi.org/10.1016/j.fsigss.2011.10.014

Australian marsupial species identification. / Wilson-Wilde, Linzi; Norman, Janette; Robertson, James; Sarre, Stephen; Georges, Arthur.

Forensic Science International: Genetics Supplement Series. Vol. 3 Netherlands : Elsevier, 2011. p. 543-544 (Genetics supplement series; Vol. 3, No. 1).

Research output: A Conference proceeding or a Chapter in BookConference contribution

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T1 - Australian marsupial species identification

AU - Wilson-Wilde, Linzi

AU - Norman, Janette

AU - Robertson, James

AU - Sarre, Stephen

AU - Georges, Arthur

PY - 2011

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N2 - Wildlife crime, the illegal trade in animals and animal products, is a growing concern and valued at up to US$20 billion globally per year. Australia is often targeted for its unique fauna, proximity to South East Asia and porous borders. Marsupials of the order Diprotodontia (including koala, wombats, possums, gliders, kangaroos) are sometimes targeted for their skin, meat and for the pet trade. However, species identification for forensic purposes must be underpinned by robust phylogenetic information. A Diprotodont phylogeny containing a large number of taxa generated from nuclear and mitochondrial data has not yet been constructed. Here the mitochondrial (COI and ND2) and nuclear markers (APOB, IRBP and GAPD) are combined to create a more robust phylogeny to underpin a species identification method for the marsupial order Diprotodontia. Mitochondrial markers were combined with nuclear markers to amplify 27 genera of Diprotodontia. Data was analysed using a likelihood method. The combined data set resolved two suborders: Vombatiformes and Phalangeriformes. Phalangeriformes was subsequently split into two clades. The first clade contained the Macropodiformes and Burramyidae. The second clade contained Petauridae, grouping with Phalangeroidea. Of the markers tested, ND2 provided the greatest level of diagnostic accuracy and could be used as a forensic species identification tool for Diprotodonts, with appropriate validation.

AB - Wildlife crime, the illegal trade in animals and animal products, is a growing concern and valued at up to US$20 billion globally per year. Australia is often targeted for its unique fauna, proximity to South East Asia and porous borders. Marsupials of the order Diprotodontia (including koala, wombats, possums, gliders, kangaroos) are sometimes targeted for their skin, meat and for the pet trade. However, species identification for forensic purposes must be underpinned by robust phylogenetic information. A Diprotodont phylogeny containing a large number of taxa generated from nuclear and mitochondrial data has not yet been constructed. Here the mitochondrial (COI and ND2) and nuclear markers (APOB, IRBP and GAPD) are combined to create a more robust phylogeny to underpin a species identification method for the marsupial order Diprotodontia. Mitochondrial markers were combined with nuclear markers to amplify 27 genera of Diprotodontia. Data was analysed using a likelihood method. The combined data set resolved two suborders: Vombatiformes and Phalangeriformes. Phalangeriformes was subsequently split into two clades. The first clade contained the Macropodiformes and Burramyidae. The second clade contained Petauridae, grouping with Phalangeroidea. Of the markers tested, ND2 provided the greatest level of diagnostic accuracy and could be used as a forensic species identification tool for Diprotodonts, with appropriate validation.

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KW - Forensic

KW - DNA

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KW - Barcoding

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U2 - 10.1016/j.fsigss.2011.10.014

DO - 10.1016/j.fsigss.2011.10.014

M3 - Conference contribution

VL - 3

T3 - Genetics supplement series

SP - 543

EP - 544

BT - Forensic Science International

PB - Elsevier

CY - Netherlands

ER -

Wilson-Wilde L, Norman J, Robertson J, Sarre S, Georges A. Australian marsupial species identification. In Forensic Science International: Genetics Supplement Series. Vol. 3. Netherlands: Elsevier. 2011. p. 543-544. (Genetics supplement series; 1). https://doi.org/10.1016/j.fsigss.2011.10.014