Bioavailability and toxicity of zinc from contaminated freshwater sediments: Linking exposure-dose-response relationships of the freshwater bivalve Hyridella australis to zinc-spiked sediments

Chamani Marasinghe Wadige, Anne TAYLOR, Bill MAHER

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    Abstract

    To evaluate the use of the freshwater bivalve Hyridella australis as a potential biomonitor for zinc con-tamination in freshwater sediments, the bioavailability and toxicity of zinc contaminated sediments (low44 ± 5, medium 526 ± 41, high 961 ± 38 g/g dry mass) were investigated in laboratory microcosms for28 days by examining H. australis exposure-dose–response relationships. Zinc concentrations in sedi-ments and surface waters were measured as zinc exposure. Zinc in whole organism soft body tissuesand five individual tissues were measured as organism zinc dose. Sub-cellular localisation of zinc in hep-atopancreas tissues was investigated to further understand the zinc handling strategies and tolerance ofH. australis. Total antioxidant capacity, lipid peroxidation and lysosomal membrane stability were measured in hepatopancreas tissues as zinc induced biomarker responses. Accumulated zinc concentrations in whole body tissues of H. australis reflected the zinc exposure and exhibited exposure dependent zincaccumulation at day 28. Gills accumulated significantly higher zinc concentrations than other tissues,however, no significant differences in zinc accumulation between treatments were detected for any of the individual tissues analysed. Analysis of individual tissue zinc concentrations, therefore, may not offer any advantages for monitoring bioavailable zinc in freshwater environments with this organism. Relationships between tissue zinc and calcium concentration suggest accumulation of zinc by H. australis may have occurred as an analogue of calcium which is a major constituent in shell and granules of unionid bivalves. A high percentage of accumulated zinc in the hepatopancreas tissues was detoxified and stored in metallothionein like proteins and metal rich granules. Of the zinc accumulated in the biologicallyactive metal pool, 59–70% was stored in the lysosome + microsome fraction. At the concentrations tested,increasing zinc exposure resulted in decreasing total antioxidant capacity and measurable increases in the sublethal effects, lipid peroxidation and lysosomal membrane destabilisation, were observed. Based on exposure-dose analysis, H. australis partially regulates zinc uptake and weakly exhibits bioavailability of zinc in freshwater environments, however, exposure-response analysis shows zinc induced toxicological effects, suggesting the potential of this organism as a biomonitor for zinc in heavily contaminated freshwater environments.
    Original languageEnglish
    Pages (from-to)179-190
    Number of pages12
    JournalAcquayic Toxicology
    Volume156
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    freshwater sediment
    Bivalvia
    dose-response relationship
    Fresh Water
    Biological Availability
    bioavailability
    dose response
    bivalve
    Zinc
    zinc
    toxicity
    sediments
    sediment
    freshwater environment
    exposure
    Hepatopancreas
    Environmental Monitoring
    organisms
    hepatopancreas
    antioxidant

    Cite this

    @article{42d6561151874ba8b1b7ff213f6aa152,
    title = "Bioavailability and toxicity of zinc from contaminated freshwater sediments: Linking exposure-dose-response relationships of the freshwater bivalve Hyridella australis to zinc-spiked sediments",
    abstract = "To evaluate the use of the freshwater bivalve Hyridella australis as a potential biomonitor for zinc con-tamination in freshwater sediments, the bioavailability and toxicity of zinc contaminated sediments (low44 ± 5, medium 526 ± 41, high 961 ± 38 g/g dry mass) were investigated in laboratory microcosms for28 days by examining H. australis exposure-dose–response relationships. Zinc concentrations in sedi-ments and surface waters were measured as zinc exposure. Zinc in whole organism soft body tissuesand five individual tissues were measured as organism zinc dose. Sub-cellular localisation of zinc in hep-atopancreas tissues was investigated to further understand the zinc handling strategies and tolerance ofH. australis. Total antioxidant capacity, lipid peroxidation and lysosomal membrane stability were measured in hepatopancreas tissues as zinc induced biomarker responses. Accumulated zinc concentrations in whole body tissues of H. australis reflected the zinc exposure and exhibited exposure dependent zincaccumulation at day 28. Gills accumulated significantly higher zinc concentrations than other tissues,however, no significant differences in zinc accumulation between treatments were detected for any of the individual tissues analysed. Analysis of individual tissue zinc concentrations, therefore, may not offer any advantages for monitoring bioavailable zinc in freshwater environments with this organism. Relationships between tissue zinc and calcium concentration suggest accumulation of zinc by H. australis may have occurred as an analogue of calcium which is a major constituent in shell and granules of unionid bivalves. A high percentage of accumulated zinc in the hepatopancreas tissues was detoxified and stored in metallothionein like proteins and metal rich granules. Of the zinc accumulated in the biologicallyactive metal pool, 59–70{\%} was stored in the lysosome + microsome fraction. At the concentrations tested,increasing zinc exposure resulted in decreasing total antioxidant capacity and measurable increases in the sublethal effects, lipid peroxidation and lysosomal membrane destabilisation, were observed. Based on exposure-dose analysis, H. australis partially regulates zinc uptake and weakly exhibits bioavailability of zinc in freshwater environments, however, exposure-response analysis shows zinc induced toxicological effects, suggesting the potential of this organism as a biomonitor for zinc in heavily contaminated freshwater environments.",
    keywords = "Biologically active zinc, Biologically detoxified zinc, Biomarkers, Freshwater bivalve, Oxidative stress, Sub-cellular partitioning",
    author = "{Marasinghe Wadige}, Chamani and Anne TAYLOR and Bill MAHER",
    year = "2014",
    doi = "10.1016/j.aquatox.2014.08.012",
    language = "English",
    volume = "156",
    pages = "179--190",
    journal = "Acquayic Toxicology",
    issn = "0166-445X",
    publisher = "Elsevier",

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    TY - JOUR

    T1 - Bioavailability and toxicity of zinc from contaminated freshwater sediments: Linking exposure-dose-response relationships of the freshwater bivalve Hyridella australis to zinc-spiked sediments

    AU - Marasinghe Wadige, Chamani

    AU - TAYLOR, Anne

    AU - MAHER, Bill

    PY - 2014

    Y1 - 2014

    N2 - To evaluate the use of the freshwater bivalve Hyridella australis as a potential biomonitor for zinc con-tamination in freshwater sediments, the bioavailability and toxicity of zinc contaminated sediments (low44 ± 5, medium 526 ± 41, high 961 ± 38 g/g dry mass) were investigated in laboratory microcosms for28 days by examining H. australis exposure-dose–response relationships. Zinc concentrations in sedi-ments and surface waters were measured as zinc exposure. Zinc in whole organism soft body tissuesand five individual tissues were measured as organism zinc dose. Sub-cellular localisation of zinc in hep-atopancreas tissues was investigated to further understand the zinc handling strategies and tolerance ofH. australis. Total antioxidant capacity, lipid peroxidation and lysosomal membrane stability were measured in hepatopancreas tissues as zinc induced biomarker responses. Accumulated zinc concentrations in whole body tissues of H. australis reflected the zinc exposure and exhibited exposure dependent zincaccumulation at day 28. Gills accumulated significantly higher zinc concentrations than other tissues,however, no significant differences in zinc accumulation between treatments were detected for any of the individual tissues analysed. Analysis of individual tissue zinc concentrations, therefore, may not offer any advantages for monitoring bioavailable zinc in freshwater environments with this organism. Relationships between tissue zinc and calcium concentration suggest accumulation of zinc by H. australis may have occurred as an analogue of calcium which is a major constituent in shell and granules of unionid bivalves. A high percentage of accumulated zinc in the hepatopancreas tissues was detoxified and stored in metallothionein like proteins and metal rich granules. Of the zinc accumulated in the biologicallyactive metal pool, 59–70% was stored in the lysosome + microsome fraction. At the concentrations tested,increasing zinc exposure resulted in decreasing total antioxidant capacity and measurable increases in the sublethal effects, lipid peroxidation and lysosomal membrane destabilisation, were observed. Based on exposure-dose analysis, H. australis partially regulates zinc uptake and weakly exhibits bioavailability of zinc in freshwater environments, however, exposure-response analysis shows zinc induced toxicological effects, suggesting the potential of this organism as a biomonitor for zinc in heavily contaminated freshwater environments.

    AB - To evaluate the use of the freshwater bivalve Hyridella australis as a potential biomonitor for zinc con-tamination in freshwater sediments, the bioavailability and toxicity of zinc contaminated sediments (low44 ± 5, medium 526 ± 41, high 961 ± 38 g/g dry mass) were investigated in laboratory microcosms for28 days by examining H. australis exposure-dose–response relationships. Zinc concentrations in sedi-ments and surface waters were measured as zinc exposure. Zinc in whole organism soft body tissuesand five individual tissues were measured as organism zinc dose. Sub-cellular localisation of zinc in hep-atopancreas tissues was investigated to further understand the zinc handling strategies and tolerance ofH. australis. Total antioxidant capacity, lipid peroxidation and lysosomal membrane stability were measured in hepatopancreas tissues as zinc induced biomarker responses. Accumulated zinc concentrations in whole body tissues of H. australis reflected the zinc exposure and exhibited exposure dependent zincaccumulation at day 28. Gills accumulated significantly higher zinc concentrations than other tissues,however, no significant differences in zinc accumulation between treatments were detected for any of the individual tissues analysed. Analysis of individual tissue zinc concentrations, therefore, may not offer any advantages for monitoring bioavailable zinc in freshwater environments with this organism. Relationships between tissue zinc and calcium concentration suggest accumulation of zinc by H. australis may have occurred as an analogue of calcium which is a major constituent in shell and granules of unionid bivalves. A high percentage of accumulated zinc in the hepatopancreas tissues was detoxified and stored in metallothionein like proteins and metal rich granules. Of the zinc accumulated in the biologicallyactive metal pool, 59–70% was stored in the lysosome + microsome fraction. At the concentrations tested,increasing zinc exposure resulted in decreasing total antioxidant capacity and measurable increases in the sublethal effects, lipid peroxidation and lysosomal membrane destabilisation, were observed. Based on exposure-dose analysis, H. australis partially regulates zinc uptake and weakly exhibits bioavailability of zinc in freshwater environments, however, exposure-response analysis shows zinc induced toxicological effects, suggesting the potential of this organism as a biomonitor for zinc in heavily contaminated freshwater environments.

    KW - Biologically active zinc

    KW - Biologically detoxified zinc

    KW - Biomarkers

    KW - Freshwater bivalve

    KW - Oxidative stress

    KW - Sub-cellular partitioning

    U2 - 10.1016/j.aquatox.2014.08.012

    DO - 10.1016/j.aquatox.2014.08.012

    M3 - Article

    VL - 156

    SP - 179

    EP - 190

    JO - Acquayic Toxicology

    JF - Acquayic Toxicology

    SN - 0166-445X

    ER -