Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals

Nicholas P. West, Claus T. Christophersen, David B. Pyne, Allan W. Cripps, Michael A. Conlon, David L. Topping, Seungha Kang, Chris S. McSweeney, Peter A. Fricker, Daniel C. Aguirre-Acevedo, Julie M. Clarke

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

BACKGROUND: Butyrate delivery to the large bowel may positively modulate commensal microbiota and enhance immunity. OBJECTIVE: To determine the effects of increasing large bowel butyrate concentration through ingestion of butyrylated high amylose maize starch (HAMSB) on faecal biochemistry and microbiota, and markers of immunity in healthy active individuals. DESIGN: Male and female volunteers were assigned randomly to consume either two doses of 20 g HAMSB (n = 23; age 37.9 +/- 7.8 y; mean +/- SD) or a low amylose maize starch (LAMS) (n = 18; age 36.9 = 9.5 y) twice daily for 28 days. Samples were collected on days 0, 10 and 28 for assessment of faecal bacterial groups, faecal biochemistry, serum cytokines and salivary antimicrobial proteins. RESULTS: HAMSB led to relative increases in faecal free (45%; 12-86%; mean; 90% confidence interval; P = 0.02), bound (950%; 563-1564%; P < 0.01) and total butyrate (260%; 174-373%; P < 0.01) and faecal propionate (41%; 12-77%; P = 0.02) from day 0 to day 28 compared to LAMS. HAMSB was also associated with a relative 1.6-fold (1.2- to 2.0-fold; P < 0.01) and 2.5-fold (1.4- to 4.4-fold; P = 0.01) increase in plasma IL-10 and TNF-alpha but did not alter other indices of immunity. There were relative greater increases in faecal P. distasonis (81-fold (28- to 237-fold; P < 0.01) and F. prausnitzii (5.1-fold (2.1- to 12-fold; P < 0.01) in the HAMSB group. CONCLUSIONS: HAMSB supplementation in healthy active individuals promotes the growth of bacteria that may improve bowel health and has only limited effects on plasma cytokines.
Original languageEnglish
Pages (from-to)102-119
Number of pages18
JournalExercise Immunology Review
Volume19
Publication statusPublished - 2013
Externally publishedYes

Cite this

West, N. P., Christophersen, C. T., Pyne, D. B., Cripps, A. W., Conlon, M. A., Topping, D. L., ... Clarke, J. M. (2013). Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals. Exercise Immunology Review, 19, 102-119.
West, Nicholas P. ; Christophersen, Claus T. ; Pyne, David B. ; Cripps, Allan W. ; Conlon, Michael A. ; Topping, David L. ; Kang, Seungha ; McSweeney, Chris S. ; Fricker, Peter A. ; Aguirre-Acevedo, Daniel C. ; Clarke, Julie M. / Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals. In: Exercise Immunology Review. 2013 ; Vol. 19. pp. 102-119.
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title = "Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals",
abstract = "BACKGROUND: Butyrate delivery to the large bowel may positively modulate commensal microbiota and enhance immunity. OBJECTIVE: To determine the effects of increasing large bowel butyrate concentration through ingestion of butyrylated high amylose maize starch (HAMSB) on faecal biochemistry and microbiota, and markers of immunity in healthy active individuals. DESIGN: Male and female volunteers were assigned randomly to consume either two doses of 20 g HAMSB (n = 23; age 37.9 +/- 7.8 y; mean +/- SD) or a low amylose maize starch (LAMS) (n = 18; age 36.9 = 9.5 y) twice daily for 28 days. Samples were collected on days 0, 10 and 28 for assessment of faecal bacterial groups, faecal biochemistry, serum cytokines and salivary antimicrobial proteins. RESULTS: HAMSB led to relative increases in faecal free (45{\%}; 12-86{\%}; mean; 90{\%} confidence interval; P = 0.02), bound (950{\%}; 563-1564{\%}; P < 0.01) and total butyrate (260{\%}; 174-373{\%}; P < 0.01) and faecal propionate (41{\%}; 12-77{\%}; P = 0.02) from day 0 to day 28 compared to LAMS. HAMSB was also associated with a relative 1.6-fold (1.2- to 2.0-fold; P < 0.01) and 2.5-fold (1.4- to 4.4-fold; P = 0.01) increase in plasma IL-10 and TNF-alpha but did not alter other indices of immunity. There were relative greater increases in faecal P. distasonis (81-fold (28- to 237-fold; P < 0.01) and F. prausnitzii (5.1-fold (2.1- to 12-fold; P < 0.01) in the HAMSB group. CONCLUSIONS: HAMSB supplementation in healthy active individuals promotes the growth of bacteria that may improve bowel health and has only limited effects on plasma cytokines.",
keywords = "Butyrylated starch, immunity, exercise, short chain fatty acids, faecal microbiota",
author = "West, {Nicholas P.} and Christophersen, {Claus T.} and Pyne, {David B.} and Cripps, {Allan W.} and Conlon, {Michael A.} and Topping, {David L.} and Seungha Kang and McSweeney, {Chris S.} and Fricker, {Peter A.} and Aguirre-Acevedo, {Daniel C.} and Clarke, {Julie M.}",
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West, NP, Christophersen, CT, Pyne, DB, Cripps, AW, Conlon, MA, Topping, DL, Kang, S, McSweeney, CS, Fricker, PA, Aguirre-Acevedo, DC & Clarke, JM 2013, 'Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals', Exercise Immunology Review, vol. 19, pp. 102-119.

Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals. / West, Nicholas P.; Christophersen, Claus T.; Pyne, David B.; Cripps, Allan W.; Conlon, Michael A.; Topping, David L.; Kang, Seungha; McSweeney, Chris S.; Fricker, Peter A.; Aguirre-Acevedo, Daniel C.; Clarke, Julie M.

In: Exercise Immunology Review, Vol. 19, 2013, p. 102-119.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Butyrylated starch increases colonic butyrate concentration but has limited effects on immunity in healthy physically active individuals

AU - West, Nicholas P.

AU - Christophersen, Claus T.

AU - Pyne, David B.

AU - Cripps, Allan W.

AU - Conlon, Michael A.

AU - Topping, David L.

AU - Kang, Seungha

AU - McSweeney, Chris S.

AU - Fricker, Peter A.

AU - Aguirre-Acevedo, Daniel C.

AU - Clarke, Julie M.

PY - 2013

Y1 - 2013

N2 - BACKGROUND: Butyrate delivery to the large bowel may positively modulate commensal microbiota and enhance immunity. OBJECTIVE: To determine the effects of increasing large bowel butyrate concentration through ingestion of butyrylated high amylose maize starch (HAMSB) on faecal biochemistry and microbiota, and markers of immunity in healthy active individuals. DESIGN: Male and female volunteers were assigned randomly to consume either two doses of 20 g HAMSB (n = 23; age 37.9 +/- 7.8 y; mean +/- SD) or a low amylose maize starch (LAMS) (n = 18; age 36.9 = 9.5 y) twice daily for 28 days. Samples were collected on days 0, 10 and 28 for assessment of faecal bacterial groups, faecal biochemistry, serum cytokines and salivary antimicrobial proteins. RESULTS: HAMSB led to relative increases in faecal free (45%; 12-86%; mean; 90% confidence interval; P = 0.02), bound (950%; 563-1564%; P < 0.01) and total butyrate (260%; 174-373%; P < 0.01) and faecal propionate (41%; 12-77%; P = 0.02) from day 0 to day 28 compared to LAMS. HAMSB was also associated with a relative 1.6-fold (1.2- to 2.0-fold; P < 0.01) and 2.5-fold (1.4- to 4.4-fold; P = 0.01) increase in plasma IL-10 and TNF-alpha but did not alter other indices of immunity. There were relative greater increases in faecal P. distasonis (81-fold (28- to 237-fold; P < 0.01) and F. prausnitzii (5.1-fold (2.1- to 12-fold; P < 0.01) in the HAMSB group. CONCLUSIONS: HAMSB supplementation in healthy active individuals promotes the growth of bacteria that may improve bowel health and has only limited effects on plasma cytokines.

AB - BACKGROUND: Butyrate delivery to the large bowel may positively modulate commensal microbiota and enhance immunity. OBJECTIVE: To determine the effects of increasing large bowel butyrate concentration through ingestion of butyrylated high amylose maize starch (HAMSB) on faecal biochemistry and microbiota, and markers of immunity in healthy active individuals. DESIGN: Male and female volunteers were assigned randomly to consume either two doses of 20 g HAMSB (n = 23; age 37.9 +/- 7.8 y; mean +/- SD) or a low amylose maize starch (LAMS) (n = 18; age 36.9 = 9.5 y) twice daily for 28 days. Samples were collected on days 0, 10 and 28 for assessment of faecal bacterial groups, faecal biochemistry, serum cytokines and salivary antimicrobial proteins. RESULTS: HAMSB led to relative increases in faecal free (45%; 12-86%; mean; 90% confidence interval; P = 0.02), bound (950%; 563-1564%; P < 0.01) and total butyrate (260%; 174-373%; P < 0.01) and faecal propionate (41%; 12-77%; P = 0.02) from day 0 to day 28 compared to LAMS. HAMSB was also associated with a relative 1.6-fold (1.2- to 2.0-fold; P < 0.01) and 2.5-fold (1.4- to 4.4-fold; P = 0.01) increase in plasma IL-10 and TNF-alpha but did not alter other indices of immunity. There were relative greater increases in faecal P. distasonis (81-fold (28- to 237-fold; P < 0.01) and F. prausnitzii (5.1-fold (2.1- to 12-fold; P < 0.01) in the HAMSB group. CONCLUSIONS: HAMSB supplementation in healthy active individuals promotes the growth of bacteria that may improve bowel health and has only limited effects on plasma cytokines.

KW - Butyrylated starch

KW - immunity

KW - exercise

KW - short chain fatty acids

KW - faecal microbiota

M3 - Article

VL - 19

SP - 102

EP - 119

JO - Exercise Immunology Review

JF - Exercise Immunology Review

SN - 1077-5552

ER -