Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing

Laura Garvican, Caroline Burge, Amanda Cox, Sally A. Clark, David Martin, Christopher Gore

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min ‘Schmidt’ and 40 min ‘Burge’ methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (tmix), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hbmass). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after tmix, and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min−1 in the Schmidt and 0.25 ± 0.16 ml min−1 in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min−1 (P= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten tmix or influence rates of CO wash-out and flux to Mb. The calculated Hbmass depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.
Original languageEnglish
Pages (from-to)1156-1166
Number of pages11
JournalExperimental Physiology
Volume95
Issue number12
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Carbon Monoxide
Myoglobin
Hemoglobins
Exhalation
Carboxyhemoglobin
Cardiovascular System
Inhalation
Healthy Volunteers
Air

Cite this

Garvican, Laura ; Burge, Caroline ; Cox, Amanda ; Clark, Sally A. ; Martin, David ; Gore, Christopher. / Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing. In: Experimental Physiology. 2010 ; Vol. 95, No. 12. pp. 1156-1166.
@article{3641c19451144a6ead6b35a47d80afd9,
title = "Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing",
abstract = "The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min ‘Schmidt’ and 40 min ‘Burge’ methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (tmix), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hbmass). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after tmix, and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min−1 in the Schmidt and 0.25 ± 0.16 ml min−1 in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min−1 (P= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten tmix or influence rates of CO wash-out and flux to Mb. The calculated Hbmass depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.",
author = "Laura Garvican and Caroline Burge and Amanda Cox and Clark, {Sally A.} and David Martin and Christopher Gore",
year = "2010",
doi = "10.1113/expphysiol.2010.054031",
language = "English",
volume = "95",
pages = "1156--1166",
journal = "Quarterly Journal of Experimental Physiology",
issn = "0958-0670",
publisher = "Wiley-Blackwell",
number = "12",

}

Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing. / Garvican, Laura; Burge, Caroline; Cox, Amanda; Clark, Sally A.; Martin, David; Gore, Christopher.

In: Experimental Physiology, Vol. 95, No. 12, 2010, p. 1156-1166.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing

AU - Garvican, Laura

AU - Burge, Caroline

AU - Cox, Amanda

AU - Clark, Sally A.

AU - Martin, David

AU - Gore, Christopher

PY - 2010

Y1 - 2010

N2 - The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min ‘Schmidt’ and 40 min ‘Burge’ methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (tmix), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hbmass). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after tmix, and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min−1 in the Schmidt and 0.25 ± 0.16 ml min−1 in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min−1 (P= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten tmix or influence rates of CO wash-out and flux to Mb. The calculated Hbmass depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.

AB - The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min ‘Schmidt’ and 40 min ‘Burge’ methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (tmix), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hbmass). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after tmix, and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min−1 in the Schmidt and 0.25 ± 0.16 ml min−1 in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min−1 (P= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten tmix or influence rates of CO wash-out and flux to Mb. The calculated Hbmass depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.

U2 - 10.1113/expphysiol.2010.054031

DO - 10.1113/expphysiol.2010.054031

M3 - Article

VL - 95

SP - 1156

EP - 1166

JO - Quarterly Journal of Experimental Physiology

JF - Quarterly Journal of Experimental Physiology

SN - 0958-0670

IS - 12

ER -