Cellular energy allocation analysis of multiple marine bivalves using near infrared spectroscopy

Jill BARTLETT, Bill MAHER, M.B.J. Purss

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This paper proposes an alternative procedure for calculating Cellular Energy Allocation (CEA) in marine bivalve species. CEA is a measure of the difference in total energy available and energy consumption and is used to assess the health of organism fitness for growth and reproduction. In this study near infra-red spectral (NIRS) quantitative data-driven modelling techniques were applied to total energy available (total protein, lipid and glycogen content) and energy consumption (electron transport system activity (ETS)) of five marine bivalve species (Saccostrea glomerata, Ostrea angasi, Crassostrea gigas, Mytilus galloprovincialis and Anadara trapezia) to produce models for measuring each bioenergetic component and calculating CEA, a biomarker of energetic stress response. Comparison of predicted to measured data showed low prediction errors (RMSEP) and high ratio of predictive error to inter-quartile distance (RPIQ) values. RMSEP for protein, lipid, glycogen and ETS models were 8.7%, 6.8%, 7.6% and 12% of the dynamic range of the validation datasets respectively while RPIQ were 3.6, 5.2, 4 and 3.7 respectively. Conversion of model outputs to energetic equivalents and calculation of total energy stores as per the CEA method demonstrate a close relationship between measured and modelled results (105%±3%). These metrics indicate that the four energy models are accurate, robust and reliable for quantitative application to all five species within the sample variation of the energetic storage measures. Applying NIRS quantitative models using freeze dried tissue eliminates water interference in NIRS capture and provides improved sample homogeneity for both spectral capture and chemical analysis. This technique enables simultaneous analyses of protein, lipid, glycogen and ETS activity to be achieved with a much higher throughput of samples (100–200 samples daily) than conventional energetic storage analysis techniques. In addition, the technique is non-destructive to tissue samples and freeze dried samples can be stored for additional analyses. The value of CEA as a response measure in S. glomerata are included, with examples of changes in CEA over four seasons and the response of oysters to a known metal contamination gradient.
Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalEcological Indicators
Volume90
DOIs
Publication statusPublished - Jul 2018

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near-infrared spectroscopy
infrared spectroscopy
bivalve
Bivalvia
near infrared
energy
energetics
Saccostrea glomerata
electron transfer
glycogen
lipid
sampling
electron
protein
lipids
Anadara
allocation
analysis
Energy
Infrared

Cite this

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title = "Cellular energy allocation analysis of multiple marine bivalves using near infrared spectroscopy",
abstract = "This paper proposes an alternative procedure for calculating Cellular Energy Allocation (CEA) in marine bivalve species. CEA is a measure of the difference in total energy available and energy consumption and is used to assess the health of organism fitness for growth and reproduction. In this study near infra-red spectral (NIRS) quantitative data-driven modelling techniques were applied to total energy available (total protein, lipid and glycogen content) and energy consumption (electron transport system activity (ETS)) of five marine bivalve species (Saccostrea glomerata, Ostrea angasi, Crassostrea gigas, Mytilus galloprovincialis and Anadara trapezia) to produce models for measuring each bioenergetic component and calculating CEA, a biomarker of energetic stress response. Comparison of predicted to measured data showed low prediction errors (RMSEP) and high ratio of predictive error to inter-quartile distance (RPIQ) values. RMSEP for protein, lipid, glycogen and ETS models were 8.7{\%}, 6.8{\%}, 7.6{\%} and 12{\%} of the dynamic range of the validation datasets respectively while RPIQ were 3.6, 5.2, 4 and 3.7 respectively. Conversion of model outputs to energetic equivalents and calculation of total energy stores as per the CEA method demonstrate a close relationship between measured and modelled results (105{\%}±3{\%}). These metrics indicate that the four energy models are accurate, robust and reliable for quantitative application to all five species within the sample variation of the energetic storage measures. Applying NIRS quantitative models using freeze dried tissue eliminates water interference in NIRS capture and provides improved sample homogeneity for both spectral capture and chemical analysis. This technique enables simultaneous analyses of protein, lipid, glycogen and ETS activity to be achieved with a much higher throughput of samples (100–200 samples daily) than conventional energetic storage analysis techniques. In addition, the technique is non-destructive to tissue samples and freeze dried samples can be stored for additional analyses. The value of CEA as a response measure in S. glomerata are included, with examples of changes in CEA over four seasons and the response of oysters to a known metal contamination gradient.",
keywords = "near infrared spectroscopy, Bivalve, Cellular energy allocation, Protein content, Lipid content, Glycogen content, electron transport system activity",
author = "Jill BARTLETT and Bill MAHER and M.B.J. Purss",
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Cellular energy allocation analysis of multiple marine bivalves using near infrared spectroscopy. / BARTLETT, Jill; MAHER, Bill; Purss, M.B.J.

In: Ecological Indicators, Vol. 90, 07.2018, p. 247-256.

Research output: Contribution to journalArticle

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AB - This paper proposes an alternative procedure for calculating Cellular Energy Allocation (CEA) in marine bivalve species. CEA is a measure of the difference in total energy available and energy consumption and is used to assess the health of organism fitness for growth and reproduction. In this study near infra-red spectral (NIRS) quantitative data-driven modelling techniques were applied to total energy available (total protein, lipid and glycogen content) and energy consumption (electron transport system activity (ETS)) of five marine bivalve species (Saccostrea glomerata, Ostrea angasi, Crassostrea gigas, Mytilus galloprovincialis and Anadara trapezia) to produce models for measuring each bioenergetic component and calculating CEA, a biomarker of energetic stress response. Comparison of predicted to measured data showed low prediction errors (RMSEP) and high ratio of predictive error to inter-quartile distance (RPIQ) values. RMSEP for protein, lipid, glycogen and ETS models were 8.7%, 6.8%, 7.6% and 12% of the dynamic range of the validation datasets respectively while RPIQ were 3.6, 5.2, 4 and 3.7 respectively. Conversion of model outputs to energetic equivalents and calculation of total energy stores as per the CEA method demonstrate a close relationship between measured and modelled results (105%±3%). These metrics indicate that the four energy models are accurate, robust and reliable for quantitative application to all five species within the sample variation of the energetic storage measures. Applying NIRS quantitative models using freeze dried tissue eliminates water interference in NIRS capture and provides improved sample homogeneity for both spectral capture and chemical analysis. This technique enables simultaneous analyses of protein, lipid, glycogen and ETS activity to be achieved with a much higher throughput of samples (100–200 samples daily) than conventional energetic storage analysis techniques. In addition, the technique is non-destructive to tissue samples and freeze dried samples can be stored for additional analyses. The value of CEA as a response measure in S. glomerata are included, with examples of changes in CEA over four seasons and the response of oysters to a known metal contamination gradient.

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KW - Cellular energy allocation

KW - Protein content

KW - Lipid content

KW - Glycogen content

KW - electron transport system activity

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