Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

S.J. Wells; W. Ji; S.M. Baillie; Dianne GLEESON

doi:10.1007/s12686-012-9745-z

Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

S.J. Wells, W. Ji, S.M. Baillie, Dianne GLEESON

Science & Technology Office

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2 Citations (Scopus)

Abstract

We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	Conservation Genetics Resources
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/s12686-012-9745-z
Publication status	Published - 2013

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@article{68a5a4f1f1c54f8195b38ca7452274c1,

title = "Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae",

abstract = "We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.",

keywords = "Microsatellite, Tui, Prosthemadera, novaeseelandiae, Honeyeater, Meliphagidae, Bellbird, Anthornis melanura, New Zealand.",

author = "S.J. Wells and W. Ji and S.M. Baillie and Dianne GLEESON",

year = "2013",

doi = "10.1007/s12686-012-9745-z",

language = "English",

volume = "5",

pages = "113--116",

journal = "Conservation Genetics Resoures",

issn = "1877-7252",

publisher = "Springer",

number = "1",

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TY - JOUR

T1 - Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

AU - Wells, S.J.

AU - Ji, W.

AU - Baillie, S.M.

AU - GLEESON, Dianne

PY - 2013

Y1 - 2013

N2 - We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

AB - We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

KW - Microsatellite

KW - Tui

KW - Prosthemadera

KW - novaeseelandiae

KW - Honeyeater

KW - Meliphagidae

KW - Bellbird

KW - Anthornis melanura

KW - New Zealand.

U2 - 10.1007/s12686-012-9745-z

DO - 10.1007/s12686-012-9745-z

M3 - Article

VL - 5

SP - 113

EP - 116

JO - Conservation Genetics Resoures

JF - Conservation Genetics Resoures

SN - 1877-7252

IS - 1

ER -

Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

Abstract

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