Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

S.J. Wells, W. Ji, S.M. Baillie, Dianne GLEESON

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.
    Original languageEnglish
    Pages (from-to)113-116
    Number of pages4
    JournalConservation Genetics Resources
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - 2013

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