Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

S.J. Wells, W. Ji, S.M. Baillie, Dianne GLEESON

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.
    Original languageEnglish
    Pages (from-to)113-116
    Number of pages4
    JournalConservation Genetics Resources
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    New Zealand
    disequilibrium
    heterozygosity
    Microsatellite Repeats
    amplification
    allele
    microsatellite repeats
    loci
    Linkage Disequilibrium
    linkage disequilibrium
    Alleles
    alleles
    marker
    Meliphagidae
    sampling

    Cite this

    @article{68a5a4f1f1c54f8195b38ca7452274c1,
    title = "Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae",
    abstract = "We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.",
    keywords = "Microsatellite, Tui, Prosthemadera, novaeseelandiae, Honeyeater, Meliphagidae, Bellbird, Anthornis melanura, New Zealand.",
    author = "S.J. Wells and W. Ji and S.M. Baillie and Dianne GLEESON",
    year = "2013",
    doi = "10.1007/s12686-012-9745-z",
    language = "English",
    volume = "5",
    pages = "113--116",
    journal = "Conservation Genetics Resoures",
    issn = "1877-7252",
    publisher = "Springer Verlag",
    number = "1",

    }

    TY - JOUR

    T1 - Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

    AU - Wells, S.J.

    AU - Ji, W.

    AU - Baillie, S.M.

    AU - GLEESON, Dianne

    PY - 2013

    Y1 - 2013

    N2 - We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

    AB - We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the crossamplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of longrepeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.

    KW - Microsatellite

    KW - Tui

    KW - Prosthemadera

    KW - novaeseelandiae

    KW - Honeyeater

    KW - Meliphagidae

    KW - Bellbird

    KW - Anthornis melanura

    KW - New Zealand.

    U2 - 10.1007/s12686-012-9745-z

    DO - 10.1007/s12686-012-9745-z

    M3 - Article

    VL - 5

    SP - 113

    EP - 116

    JO - Conservation Genetics Resoures

    JF - Conservation Genetics Resoures

    SN - 1877-7252

    IS - 1

    ER -