Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri

Chris Clay; Dianne Gleeson; Robyn Howitt; Hayley Lawrence; Jawad Abdelkrim; Neil Gemmell

doi:10.1007/s12686-010-9211-8

Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri

Chris Clay, Dianne Gleeson, Robyn Howitt, Hayley Lawrence, Jawad Abdelkrim, Neil Gemmell

Science

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Abstract

Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.

Original language	English
Pages (from-to)	301-303
Number of pages	3
Journal	Conservation Genetics Resources
Volume	2
DOIs	https://doi.org/10.1007/s12686-010-9211-8
Publication status	Published - 2010

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abstract = "Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.",

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AU - Clay, Chris

AU - Gleeson, Dianne

AU - Howitt, Robyn

AU - Lawrence, Hayley

AU - Abdelkrim, Jawad

AU - Gemmell, Neil

PY - 2010

Y1 - 2010

N2 - Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.

AB - Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.

KW - Microsatellites

KW - Leiopelma

KW - Endemic

KW - Genomic sequencing

KW - New Zealand.

U2 - 10.1007/s12686-010-9211-8

DO - 10.1007/s12686-010-9211-8

M3 - Article

SN - 1877-7252

VL - 2

SP - 301

EP - 303

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

ER -

Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri

Abstract

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