Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri

Chris Clay, Dianne Gleeson, Robyn Howitt, Hayley Lawrence, Jawad Abdelkrim, Neil Gemmell

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.
    Original languageEnglish
    Pages (from-to)301-303
    Number of pages3
    JournalConservation Genetics Resources
    Volume2
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    New Zealand
    frog
    Anura
    Microsatellite Repeats
    frogs
    microsatellite repeats
    amplification
    genomics
    new species
    Technology
    genetic variation
    loci
    marker

    Cite this

    Clay, Chris ; Gleeson, Dianne ; Howitt, Robyn ; Lawrence, Hayley ; Abdelkrim, Jawad ; Gemmell, Neil. / Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri. In: Conservation Genetics Resources. 2010 ; Vol. 2. pp. 301-303.
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    abstract = "Hochstetters frog (Leiopelma hochstetteri) is one of only four endemic frog species within New Zealand. Here we describe the first 11 polymorphic microsatellite loci for this species developed using high-throughput genomic sequencing technology. Genetic diversity was assessed in 34 individuals sampled from a single location. We also tested these in the critically threatened L. hamiltoni, which may number fewer than 150 frogs, with six markers showing amplification.",
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    author = "Chris Clay and Dianne Gleeson and Robyn Howitt and Hayley Lawrence and Jawad Abdelkrim and Neil Gemmell",
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    doi = "10.1007/s12686-010-9211-8",
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    Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri. / Clay, Chris; Gleeson, Dianne; Howitt, Robyn; Lawrence, Hayley; Abdelkrim, Jawad; Gemmell, Neil.

    In: Conservation Genetics Resources, Vol. 2, 2010, p. 301-303.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Characterisation of microsatellite markers for the primitive New Zealand frog, Leiopelma hochstetteri

    AU - Clay, Chris

    AU - Gleeson, Dianne

    AU - Howitt, Robyn

    AU - Lawrence, Hayley

    AU - Abdelkrim, Jawad

    AU - Gemmell, Neil

    PY - 2010

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    KW - Microsatellites

    KW - Leiopelma

    KW - Endemic

    KW - Genomic sequencing

    KW - New Zealand.

    U2 - 10.1007/s12686-010-9211-8

    DO - 10.1007/s12686-010-9211-8

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