Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

A Bhattacharya; R Ghildyal; S Bhattacharya; L S Diamond

Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

A Bhattacharya, R Ghildyal, S Bhattacharya, L S Diamond

Research output: Contribution to journal › Article › peer-review

Abstract

Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

Original language	English
Pages (from-to)	3458-3461
Number of pages	4
Journal	Infection and Immunity
Volume	58
Issue number	10
Publication status	Published - Oct 1990
Externally published	Yes

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title = "Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates",

abstract = "Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.",

keywords = "Animals, Antibodies, Bacterial, Antibodies, Monoclonal, Antibody Specificity, Antigens, Protozoan, Blotting, Western, Carbohydrates, Entamoeba histolytica, Enzyme-Linked Immunosorbent Assay, Epitopes, Mice, Mice, Inbred BALB C, Journal Article, Research Support, Non-U.S. Gov't",

author = "A Bhattacharya and R Ghildyal and S Bhattacharya and Diamond, {L S}",

year = "1990",

month = oct,

language = "English",

volume = "58",

pages = "3458--3461",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "10",

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TY - JOUR

T1 - Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

AU - Bhattacharya, A

AU - Ghildyal, R

AU - Bhattacharya, S

AU - Diamond, L S

PY - 1990/10

Y1 - 1990/10

N2 - Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

AB - Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

KW - Animals

KW - Antibodies, Bacterial

KW - Antibodies, Monoclonal

KW - Antibody Specificity

KW - Antigens, Protozoan

KW - Blotting, Western

KW - Carbohydrates

KW - Entamoeba histolytica

KW - Enzyme-Linked Immunosorbent Assay

KW - Epitopes

KW - Mice

KW - Mice, Inbred BALB C

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 1698182

SN - 0019-9567

VL - 58

SP - 3458

EP - 3461

JO - Infection and Immunity

JF - Infection and Immunity

IS - 10

ER -

Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

Abstract

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