Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

A Bhattacharya, R Ghildyal, S Bhattacharya, L S Diamond

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

Original languageEnglish
Pages (from-to)3458-3461
Number of pages4
JournalInfection and Immunity
Volume58
Issue number10
Publication statusPublished - Oct 1990
Externally publishedYes

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Entamoeba histolytica
Monoclonal Antibodies
Antigens
Western Blotting
Entamoeba
Trypsin
Peptide Hydrolases
Carbohydrates

Cite this

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title = "Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates",
abstract = "Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.",
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author = "A Bhattacharya and R Ghildyal and S Bhattacharya and Diamond, {L S}",
year = "1990",
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language = "English",
volume = "58",
pages = "3458--3461",
journal = "Infection and Immunity",
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Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates. / Bhattacharya, A; Ghildyal, R; Bhattacharya, S; Diamond, L S.

In: Infection and Immunity, Vol. 58, No. 10, 10.1990, p. 3458-3461.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates

AU - Bhattacharya, A

AU - Ghildyal, R

AU - Bhattacharya, S

AU - Diamond, L S

PY - 1990/10

Y1 - 1990/10

N2 - Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

AB - Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.

KW - Animals

KW - Antibodies, Bacterial

KW - Antibodies, Monoclonal

KW - Antibody Specificity

KW - Antigens, Protozoan

KW - Blotting, Western

KW - Carbohydrates

KW - Entamoeba histolytica

KW - Enzyme-Linked Immunosorbent Assay

KW - Epitopes

KW - Mice

KW - Mice, Inbred BALB C

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 58

SP - 3458

EP - 3461

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 10

ER -