Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis

Luana Bontempo; Matteo Perini; Silvia Pianezze; Micha Horacek; Andreas Roßmann; Simon D. Kelly; Freddy Thomas; Katharina Heinrich; Claus Schlicht; Antje Schellenberg; Jurian Hoogewerff; Gerhard Heiss; Bernhard Wimmer; Federica Camin

doi:10.3390/molecules28062856

Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis

Luana Bontempo, Matteo Perini, Silvia Pianezze, Micha Horacek, Andreas Roßmann, Simon D. Kelly, Freddy Thomas, Katharina Heinrich, Claus Schlicht, Antje Schellenberg, Jurian Hoogewerff, Gerhard Heiss, Bernhard Wimmer, Federica Camin

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Abstract

The need to guarantee the geographical origin of food samples has become imperative in recent years due to the increasing amount of food fraud. Stable isotope ratio analysis permits the characterization and origin control of foodstuffs, thanks to its capability to discriminate between products having different geographical origins and derived from different production systems. The Framework 6 EU-project “TRACE” generated hydrogen (²H/¹H), carbon (¹³C/¹²C), nitrogen (¹⁵N/¹⁴N), and sulphur (³⁴S/³²S) isotope ratio data from 227 authentic beef samples. These samples were collected from a total of 13 sites in eight countries. The stable isotope analysis was completed by combining IRMS with a thermal conversion elemental analyzer (TC/EA) for the analysis of δ(²H) and an elemental analyzer (EA) for the determination of δ(¹³C), δ(¹⁵N), and δ(³⁴S). The results show the potential of this technique to detect clustering of samples due to specific environmental conditions in the areas where the beef cattle were reared. Stable isotope measurements highlighted statistical differences between coastal and inland regions, production sites at different latitudes, regions with different geology, and different farming systems related to the diet the animals were consuming (primarily C3- or C4-based or a mixed one).

Original language	English
Article number	2856
Pages (from-to)	1-13
Number of pages	13
Journal	Molecules
Volume	28
Issue number	6
DOIs	https://doi.org/10.3390/molecules28062856
Publication status	Published - 22 Mar 2023

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Bontempo, L., Perini, M., Pianezze, S., Horacek, M., Roßmann, A., Kelly, S. D., Thomas, F., Heinrich, K., Schlicht, C., Schellenberg, A., Hoogewerff, J., Heiss, G., Wimmer, B., & Camin, F. (2023). Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis. Molecules, 28(6), 1-13. Article 2856. https://doi.org/10.3390/molecules28062856

@article{e921e30d34214dbbb3987b27b11f3947,

title = "Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis",

abstract = "The need to guarantee the geographical origin of food samples has become imperative in recent years due to the increasing amount of food fraud. Stable isotope ratio analysis permits the characterization and origin control of foodstuffs, thanks to its capability to discriminate between products having different geographical origins and derived from different production systems. The Framework 6 EU-project “TRACE” generated hydrogen (2H/1H), carbon (13C/12C), nitrogen (15N/14N), and sulphur (34S/32S) isotope ratio data from 227 authentic beef samples. These samples were collected from a total of 13 sites in eight countries. The stable isotope analysis was completed by combining IRMS with a thermal conversion elemental analyzer (TC/EA) for the analysis of δ(2H) and an elemental analyzer (EA) for the determination of δ(13C), δ(15N), and δ(34S). The results show the potential of this technique to detect clustering of samples due to specific environmental conditions in the areas where the beef cattle were reared. Stable isotope measurements highlighted statistical differences between coastal and inland regions, production sites at different latitudes, regions with different geology, and different farming systems related to the diet the animals were consuming (primarily C3- or C4-based or a mixed one).",

keywords = "authenticity, beef, food fraud, geographical origin, meat, stable isotope ratio analysis",

author = "Luana Bontempo and Matteo Perini and Silvia Pianezze and Micha Horacek and Andreas Ro{\ss}mann and Kelly, {Simon D.} and Freddy Thomas and Katharina Heinrich and Claus Schlicht and Antje Schellenberg and Jurian Hoogewerff and Gerhard Heiss and Bernhard Wimmer and Federica Camin",

note = "Funding Information: This work was funded wholly or in part by the European Commission, under the FP6 Food Quality and Safety Priority, within the framework of the Integrated Project TRACE—006942—entitled “Tracing Food Commodities in Europe”. The information contained in this paper reflects the authors{\textquoteright} views; the European Commission is not liable for any use of the information contained therein. Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = mar,

day = "22",

doi = "10.3390/molecules28062856",

language = "English",

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journal = "Molecules",

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Bontempo, L, Perini, M, Pianezze, S, Horacek, M, Roßmann, A, Kelly, SD, Thomas, F, Heinrich, K, Schlicht, C, Schellenberg, A, Hoogewerff, J, Heiss, G, Wimmer, B & Camin, F 2023, 'Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis', Molecules, vol. 28, no. 6, 2856, pp. 1-13. https://doi.org/10.3390/molecules28062856

TY - JOUR

T1 - Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis

AU - Bontempo, Luana

AU - Perini, Matteo

AU - Pianezze, Silvia

AU - Horacek, Micha

AU - Roßmann, Andreas

AU - Kelly, Simon D.

AU - Thomas, Freddy

AU - Heinrich, Katharina

AU - Schlicht, Claus

AU - Schellenberg, Antje

AU - Hoogewerff, Jurian

AU - Heiss, Gerhard

AU - Wimmer, Bernhard

AU - Camin, Federica

N1 - Funding Information: This work was funded wholly or in part by the European Commission, under the FP6 Food Quality and Safety Priority, within the framework of the Integrated Project TRACE—006942—entitled “Tracing Food Commodities in Europe”. The information contained in this paper reflects the authors’ views; the European Commission is not liable for any use of the information contained therein. Publisher Copyright: © 2023 by the authors.

PY - 2023/3/22

Y1 - 2023/3/22

N2 - The need to guarantee the geographical origin of food samples has become imperative in recent years due to the increasing amount of food fraud. Stable isotope ratio analysis permits the characterization and origin control of foodstuffs, thanks to its capability to discriminate between products having different geographical origins and derived from different production systems. The Framework 6 EU-project “TRACE” generated hydrogen (2H/1H), carbon (13C/12C), nitrogen (15N/14N), and sulphur (34S/32S) isotope ratio data from 227 authentic beef samples. These samples were collected from a total of 13 sites in eight countries. The stable isotope analysis was completed by combining IRMS with a thermal conversion elemental analyzer (TC/EA) for the analysis of δ(2H) and an elemental analyzer (EA) for the determination of δ(13C), δ(15N), and δ(34S). The results show the potential of this technique to detect clustering of samples due to specific environmental conditions in the areas where the beef cattle were reared. Stable isotope measurements highlighted statistical differences between coastal and inland regions, production sites at different latitudes, regions with different geology, and different farming systems related to the diet the animals were consuming (primarily C3- or C4-based or a mixed one).

AB - The need to guarantee the geographical origin of food samples has become imperative in recent years due to the increasing amount of food fraud. Stable isotope ratio analysis permits the characterization and origin control of foodstuffs, thanks to its capability to discriminate between products having different geographical origins and derived from different production systems. The Framework 6 EU-project “TRACE” generated hydrogen (2H/1H), carbon (13C/12C), nitrogen (15N/14N), and sulphur (34S/32S) isotope ratio data from 227 authentic beef samples. These samples were collected from a total of 13 sites in eight countries. The stable isotope analysis was completed by combining IRMS with a thermal conversion elemental analyzer (TC/EA) for the analysis of δ(2H) and an elemental analyzer (EA) for the determination of δ(13C), δ(15N), and δ(34S). The results show the potential of this technique to detect clustering of samples due to specific environmental conditions in the areas where the beef cattle were reared. Stable isotope measurements highlighted statistical differences between coastal and inland regions, production sites at different latitudes, regions with different geology, and different farming systems related to the diet the animals were consuming (primarily C3- or C4-based or a mixed one).

KW - authenticity

KW - beef

KW - food fraud

KW - geographical origin

KW - meat

KW - stable isotope ratio analysis

UR - http://www.scopus.com/inward/record.url?scp=85151112515&partnerID=8YFLogxK

U2 - 10.3390/molecules28062856

DO - 10.3390/molecules28062856

M3 - Article

C2 - 36985828

AN - SCOPUS:85151112515

SN - 1420-3049

VL - 28

SP - 1

EP - 13

JO - Molecules

JF - Molecules

IS - 6

M1 - 2856

ER -

Characterization of Beef Coming from Different European Countries through Stable Isotope (H, C, N, and S) Ratio Analysis

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