Characterization of Ross River virus tropism and virus-induced inflammation in a mouse model of viral arthritis and myositis

Tom Morrison, Alan Whitmore, Reed Shabman, Brett Lidbury, Suresh Mahalingam, Mark Heise

    Research output: Contribution to journalArticle

    121 Citations (Scopus)

    Abstract

    Mosquito-borne alphaviruses are a significant cause of both encephalitic and arthritic disease in humans worldwide. In contrast to the encephalitic alphaviruses, the pathogenesis of alphavirus-induced arthritic disease is not well understood. Utilizing a mouse model of Ross River virus (RRV) disease, we found that the primary targets of RRV infection are bone, joint, and skeletal muscle tissues of the hind limbs in both outbred CD-1 mice and adult C57BL/6J mice. Moreover, histological analyses demonstrated that RRV infection resulted in severe inflammation of these tissues. Characterization of the inflammatory infiltrate within the skeletal muscle tissue identified inflammatory macrophages, NK cells, and CD4+ and CD8+ T lymphocytes. To determine the contribution of the adaptive immune system, the outcome of RRV-induced disease was examined in C57BL/6J RAG-1(-/-) mice, which lack functional T and B lymphocytes. RAG-1(-/-) and wild-type mice developed similar disease signs, infiltration of inflammatory macrophages and NK cells, and muscle pathology, suggesting that the adaptive immune response does not play a critical role in the development of disease. These results establish the mouse model of RRV disease as a useful system for the identification of viral and host factors that contribute to alphavirus-induced arthritis and myositis.
    Original languageEnglish
    Pages (from-to)737-749
    Number of pages13
    JournalJournal of Virology
    Volume80
    Issue number2
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Ross River virus
    myositis
    tropisms
    Infectious Arthritis
    Myositis
    Tropism
    Alphavirus
    arthritis
    Virus Diseases
    inflammation
    animal models
    Viruses
    Inflammation
    viruses
    Arthritis
    mice
    natural killer cells
    muscle tissues
    Natural Killer Cells
    Muscles

    Cite this

    Morrison, Tom ; Whitmore, Alan ; Shabman, Reed ; Lidbury, Brett ; Mahalingam, Suresh ; Heise, Mark. / Characterization of Ross River virus tropism and virus-induced inflammation in a mouse model of viral arthritis and myositis. In: Journal of Virology. 2006 ; Vol. 80, No. 2. pp. 737-749.
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    abstract = "Mosquito-borne alphaviruses are a significant cause of both encephalitic and arthritic disease in humans worldwide. In contrast to the encephalitic alphaviruses, the pathogenesis of alphavirus-induced arthritic disease is not well understood. Utilizing a mouse model of Ross River virus (RRV) disease, we found that the primary targets of RRV infection are bone, joint, and skeletal muscle tissues of the hind limbs in both outbred CD-1 mice and adult C57BL/6J mice. Moreover, histological analyses demonstrated that RRV infection resulted in severe inflammation of these tissues. Characterization of the inflammatory infiltrate within the skeletal muscle tissue identified inflammatory macrophages, NK cells, and CD4+ and CD8+ T lymphocytes. To determine the contribution of the adaptive immune system, the outcome of RRV-induced disease was examined in C57BL/6J RAG-1(-/-) mice, which lack functional T and B lymphocytes. RAG-1(-/-) and wild-type mice developed similar disease signs, infiltration of inflammatory macrophages and NK cells, and muscle pathology, suggesting that the adaptive immune response does not play a critical role in the development of disease. These results establish the mouse model of RRV disease as a useful system for the identification of viral and host factors that contribute to alphavirus-induced arthritis and myositis.",
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    Characterization of Ross River virus tropism and virus-induced inflammation in a mouse model of viral arthritis and myositis. / Morrison, Tom; Whitmore, Alan; Shabman, Reed; Lidbury, Brett; Mahalingam, Suresh; Heise, Mark.

    In: Journal of Virology, Vol. 80, No. 2, 2006, p. 737-749.

    Research output: Contribution to journalArticle

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