Characterization of the karyotype and accumulation of repetitive sequences in Australian Darling hardyhead Craterocephalus amniculus (Atheriniformes, Teleostei)

Zuzana Majtánová, Karl G. Moy, Peter J. Unmack, Petr Ráb, Tariq Ezaz

Research output: Contribution to journalArticle

Abstract

Belonging to the order Atheriniformes, Craterocephalus is one of the most widespread genera of freshwater fishes in Australia, spanning along the northern coast from central Western Australia to central New South Wales and across the Murray-Darling and Lake Eyre basins. In this study, both conventional cytogenetic techniques (Giemsa, C-banding, CMA 3 /DAPI staining), and fluorescence in situ hybridization (FISH) with telomeric DNA and rDNA probes were used to examine the karyotypes and other chromosomal characteristics of Darling hardyhead ( Craterocephalus amniculus ) from New South Wales, Australia. We identified a diploid chromosome number 2 n  = 48 ( NF  = 58) in all studied individuals. FISH with rDNA probes showed a nonsyntenic pattern, with signals on one pair of subtelocentric chromosomes for 5S rDNA and one pair of submetacentric chromosomes for 28S rDNA. C-banding displayed the accumulation of constitutive heterochromatin in the centromeric regions of approximately 40 chromosomes. CMA 3 /DAPI fluorescence staining revealed extremely GC-rich signals in the pericentromeric region of one submetacentric chromosomal pair with size polymorphism. We detected telomeric signals at the end of all chromosomes and no interstitial signals.
Original languageEnglish
Article numbere7347
Pages (from-to)1-9
Number of pages9
JournalPEERJ
Volume7
DOIs
Publication statusPublished - 30 Jul 2019

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Atheriniformes
repetitive sequences
Nucleic Acid Repetitive Sequences
Chromosomes
Ribosomal DNA
Karyotype
karyotyping
chromosome banding
fluorescence in situ hybridization
chromosomes
New South Wales
Fluorescence In Situ Hybridization
Fluorescence
submetacentric chromosomes
heterochromatin
Staining and Labeling
cytogenetics
freshwater fish
Western Australia
South Australia

Cite this

@article{68288c2fd1854f19bf45704c633e2ec5,
title = "Characterization of the karyotype and accumulation of repetitive sequences in Australian Darling hardyhead Craterocephalus amniculus (Atheriniformes, Teleostei)",
abstract = "Belonging to the order Atheriniformes, Craterocephalus is one of the most widespread genera of freshwater fishes in Australia, spanning along the northern coast from central Western Australia to central New South Wales and across the Murray-Darling and Lake Eyre basins. In this study, both conventional cytogenetic techniques (Giemsa, C-banding, CMA 3 /DAPI staining), and fluorescence in situ hybridization (FISH) with telomeric DNA and rDNA probes were used to examine the karyotypes and other chromosomal characteristics of Darling hardyhead ( Craterocephalus amniculus ) from New South Wales, Australia. We identified a diploid chromosome number 2 n  = 48 ( NF  = 58) in all studied individuals. FISH with rDNA probes showed a nonsyntenic pattern, with signals on one pair of subtelocentric chromosomes for 5S rDNA and one pair of submetacentric chromosomes for 28S rDNA. C-banding displayed the accumulation of constitutive heterochromatin in the centromeric regions of approximately 40 chromosomes. CMA 3 /DAPI fluorescence staining revealed extremely GC-rich signals in the pericentromeric region of one submetacentric chromosomal pair with size polymorphism. We detected telomeric signals at the end of all chromosomes and no interstitial signals.",
keywords = "Fish cytotaxonomy, Karyotype, FISH, rDNA, Chromosomal markers, Atherinidae, Heterochromatin, Telomeres, Chromosomes",
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year = "2019",
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language = "English",
volume = "7",
pages = "1--9",
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Characterization of the karyotype and accumulation of repetitive sequences in Australian Darling hardyhead Craterocephalus amniculus (Atheriniformes, Teleostei). / Majtánová, Zuzana; Moy, Karl G.; Unmack, Peter J.; Ráb, Petr; Ezaz, Tariq.

In: PEERJ, Vol. 7, e7347, 30.07.2019, p. 1-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of the karyotype and accumulation of repetitive sequences in Australian Darling hardyhead Craterocephalus amniculus (Atheriniformes, Teleostei)

AU - Majtánová, Zuzana

AU - Moy, Karl G.

AU - Unmack, Peter J.

AU - Ráb, Petr

AU - Ezaz, Tariq

PY - 2019/7/30

Y1 - 2019/7/30

N2 - Belonging to the order Atheriniformes, Craterocephalus is one of the most widespread genera of freshwater fishes in Australia, spanning along the northern coast from central Western Australia to central New South Wales and across the Murray-Darling and Lake Eyre basins. In this study, both conventional cytogenetic techniques (Giemsa, C-banding, CMA 3 /DAPI staining), and fluorescence in situ hybridization (FISH) with telomeric DNA and rDNA probes were used to examine the karyotypes and other chromosomal characteristics of Darling hardyhead ( Craterocephalus amniculus ) from New South Wales, Australia. We identified a diploid chromosome number 2 n  = 48 ( NF  = 58) in all studied individuals. FISH with rDNA probes showed a nonsyntenic pattern, with signals on one pair of subtelocentric chromosomes for 5S rDNA and one pair of submetacentric chromosomes for 28S rDNA. C-banding displayed the accumulation of constitutive heterochromatin in the centromeric regions of approximately 40 chromosomes. CMA 3 /DAPI fluorescence staining revealed extremely GC-rich signals in the pericentromeric region of one submetacentric chromosomal pair with size polymorphism. We detected telomeric signals at the end of all chromosomes and no interstitial signals.

AB - Belonging to the order Atheriniformes, Craterocephalus is one of the most widespread genera of freshwater fishes in Australia, spanning along the northern coast from central Western Australia to central New South Wales and across the Murray-Darling and Lake Eyre basins. In this study, both conventional cytogenetic techniques (Giemsa, C-banding, CMA 3 /DAPI staining), and fluorescence in situ hybridization (FISH) with telomeric DNA and rDNA probes were used to examine the karyotypes and other chromosomal characteristics of Darling hardyhead ( Craterocephalus amniculus ) from New South Wales, Australia. We identified a diploid chromosome number 2 n  = 48 ( NF  = 58) in all studied individuals. FISH with rDNA probes showed a nonsyntenic pattern, with signals on one pair of subtelocentric chromosomes for 5S rDNA and one pair of submetacentric chromosomes for 28S rDNA. C-banding displayed the accumulation of constitutive heterochromatin in the centromeric regions of approximately 40 chromosomes. CMA 3 /DAPI fluorescence staining revealed extremely GC-rich signals in the pericentromeric region of one submetacentric chromosomal pair with size polymorphism. We detected telomeric signals at the end of all chromosomes and no interstitial signals.

KW - Fish cytotaxonomy

KW - Karyotype

KW - FISH

KW - rDNA

KW - Chromosomal markers

KW - Atherinidae

KW - Heterochromatin

KW - Telomeres

KW - Chromosomes

UR - http://www.mendeley.com/research/characterization-karyotype-accumulation-repetitive-sequences-australian-darling-hardyhead-craterocep

U2 - 10.7717/peerj.7347

DO - 10.7717/peerj.7347

M3 - Article

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SP - 1

EP - 9

JO - PEERJ

JF - PEERJ

SN - 2167-8359

M1 - e7347

ER -