Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial

T Urashima, Tomoko Yamamoto, K Hirayama, K Fukuda, T Nakamura, T Saito, Keith Newgrain, J Merchant, Brian Green, M Messer

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    Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by 1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–3)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Neu5Ac(α2–3) Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with an α(2–3)Neu5Ac linked to β(1–4)Gal residue of either branch of Gal(β1–4)GlcNAc(β1–6) units, and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with a β(1–3) linked Gal and an α(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–4)Glc and their α(2–3) linked Neu5Ac derivatives.

    Original languageEnglish
    Pages (from-to)797-807
    Number of pages11
    JournalGlycoconjugate Journal
    Issue number5
    Publication statusPublished - 1 Oct 2016


    Cite this

    Urashima, T., Yamamoto, T., Hirayama, K., Fukuda, K., Nakamura, T., Saito, T., ... Messer, M. (2016). Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial. Glycoconjugate Journal, 33(5), 797-807.