### Abstract

Original language | English |
---|---|

Pages (from-to) | 797-807 |

Number of pages | 11 |

Journal | Glycoconjugate Journal |

Volume | 33 |

Issue number | 5 |

DOIs | |

Publication status | Published - 2016 |

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*Glycoconjugate Journal*,

*33*(5), 797-807. https://doi.org/10.1007/s10719-016-9675-1

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*Glycoconjugate Journal*, vol. 33, no. 5, pp. 797-807. https://doi.org/10.1007/s10719-016-9675-1

**Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial.** / Urashima, T; Yamamoto, Tomoko; Hirayama, K; Fukuda, K; Nakamura, T; Saito, T; Newgrain, Keith; Merchant, J; Green, Brian; Messer, M.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial

AU - Urashima, T

AU - Yamamoto, Tomoko

AU - Hirayama, K

AU - Fukuda, K

AU - Nakamura, T

AU - Saito, T

AU - Newgrain, Keith

AU - Merchant, J

AU - Green, Brian

AU - Messer, M

PY - 2016

Y1 - 2016

N2 - Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by 1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–3)Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Neu5Ac(a2–3) Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc with an a(2–3)Neu5Ac linked to ß(1–4)Gal residue of either branch of Gal(ß1–4)GlcNAc(ß1–6) units, and Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc with a ß(1–3) linked Gal and an a(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(ß1–3){Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)GlcNAc(ß1–6)}Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc and Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3){Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)GlcNAc(ß1–6)}Gal(ß1–4)Glc and their a(2–3) linked Neu5Ac derivatives.

AB - Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by 1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–3)Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Neu5Ac(a2–3) Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc, Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc with an a(2–3)Neu5Ac linked to ß(1–4)Gal residue of either branch of Gal(ß1–4)GlcNAc(ß1–6) units, and Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc with a ß(1–3) linked Gal and an a(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(ß1–3){Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)GlcNAc(ß1–6)}Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)Glc and Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–3){Gal(ß1–3)[Gal(ß1–4)GlcNAc(ß1–6)]Gal(ß1–4)GlcNAc(ß1–6)}Gal(ß1–4)Glc and their a(2–3) linked Neu5Ac derivatives.

KW - Marsupials

KW - Milk oligosaccharides

KW - Tiger quoll

U2 - 10.1007/s10719-016-9675-1

DO - 10.1007/s10719-016-9675-1

M3 - Article

VL - 33

SP - 797

EP - 807

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

IS - 5

ER -