TY - JOUR
T1 - Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial
AU - Urashima, T
AU - Yamamoto, Tomoko
AU - Hirayama, K
AU - Fukuda, K
AU - Nakamura, T
AU - Saito, T
AU - Newgrain, Keith
AU - Merchant, J
AU - Green, Brian
AU - Messer, M
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by
1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–3)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Neu5Ac(α2–3) Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with an α(2–3)Neu5Ac linked to β(1–4)Gal residue of either branch of Gal(β1–4)GlcNAc(β1–6) units, and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with a β(1–3) linked Gal and an α(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–4)Glc and their α(2–3) linked Neu5Ac derivatives.
AB - Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by
1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–3)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Neu5Ac(α2–3) Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with an α(2–3)Neu5Ac linked to β(1–4)Gal residue of either branch of Gal(β1–4)GlcNAc(β1–6) units, and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with a β(1–3) linked Gal and an α(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–4)Glc and their α(2–3) linked Neu5Ac derivatives.
KW - Marsupials
KW - Milk oligosaccharides
KW - Tiger quoll
UR - http://www.scopus.com/inward/record.url?scp=84969963092&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/chemical-characterization-milk-oligosaccharides-tiger-quoll-dasyurus-maculatus-marsupial
U2 - 10.1007/s10719-016-9675-1
DO - 10.1007/s10719-016-9675-1
M3 - Article
SN - 0282-0080
VL - 33
SP - 797
EP - 807
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 5
ER -