Saffron, the dried red stigmas of Crocus sativus L. is the world's most expensive spice. The expense of Saffron has led to greater incidences of fraudulent practice within this market. The importation and rebranding of cheaper growing regions to be passed off as regions of higher quality is one such practice. Analytical techniques have been reported in literature for rapid detection of these fraud issues. These techniques can be affected by post harvest processing, therefore more confirmatory techniques with direct links to the environment should also be considered. Trace element concentrations and stable isotopic ratios are investigated in this study to differentiate the geographic origins of Saffron grown in Khorasan Province, Iran and the La Mancha region of Spain. Statistical analysis of the data using Linear Discriminate Analysis (LDA) resulted in a classification model which could determine saffron from each origin, suggesting this is a feasible confirmatory approach for origin verification of Saffron.