Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

Olivia Leitch, Alexie Papanicolaou, Chris Lennard, Paul Kirkbride, Alisha Anderson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results: Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions: This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.
Original languageEnglish
Article number255
Pages (from-to)1-17
Number of pages17
JournalBMC Genomics
Volume16
Issue number1
DOIs
Publication statusPublished - 31 Mar 2015

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Smell
Transcriptome
Odorant Receptors
Genes
Research
Insecticide Resistance
Forensic Sciences
Pest Control
Proteins
Forensic Medicine
Aptitude
Essential Genes
Sensory Receptor Cells
Esterases
Cytochromes
Agriculture
Diptera
Larva
Insects
Membrane Proteins

Cite this

Leitch, O., Papanicolaou, A., Lennard, C., Kirkbride, P., & Anderson, A. (2015). Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia. BMC Genomics, 16(1), 1-17. [255]. https://doi.org/10.1186/s12864-015-1466-8
Leitch, Olivia ; Papanicolaou, Alexie ; Lennard, Chris ; Kirkbride, Paul ; Anderson, Alisha. / Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia. In: BMC Genomics. 2015 ; Vol. 16, No. 1. pp. 1-17.
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Leitch, O, Papanicolaou, A, Lennard, C, Kirkbride, P & Anderson, A 2015, 'Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia', BMC Genomics, vol. 16, no. 1, 255, pp. 1-17. https://doi.org/10.1186/s12864-015-1466-8

Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia. / Leitch, Olivia; Papanicolaou, Alexie; Lennard, Chris; Kirkbride, Paul; Anderson, Alisha.

In: BMC Genomics, Vol. 16, No. 1, 255, 31.03.2015, p. 1-17.

Research output: Contribution to journalArticle

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T1 - Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

AU - Leitch, Olivia

AU - Papanicolaou, Alexie

AU - Lennard, Chris

AU - Kirkbride, Paul

AU - Anderson, Alisha

PY - 2015/3/31

Y1 - 2015/3/31

N2 - Background: Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results: Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions: This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.

AB - Background: Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results: Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions: This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.

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KW - Chemosensory proteins

KW - Odorant receptors

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