Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata).

Kornsorn Srikulnath, Yoshinobu Uno, Kazumi Matsubara, Amara Thongpan, Saowanee Suputtitada, Somsak Apisitwanich, Chizuko Nishida, Yoichi Matsuda

    Research output: Contribution to journalArticle

    Abstract

    Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)
    Original languageEnglish
    Pages (from-to)583-586
    Number of pages4
    JournalGenetics and Molecular Biology
    Volume34
    Issue number4
    DOIs
    Publication statusPublished - 2011

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    Butterflies
    Lizards
    rRNA Genes
    Chromosomes, Human, Pair 1
    Telomere
    Constriction
    Chromosomes
    Chromosomes, Human, Pair 6
    Protein Sorting Signals
    Karyotype

    Cite this

    Srikulnath, Kornsorn ; Uno, Yoshinobu ; Matsubara, Kazumi ; Thongpan, Amara ; Suputtitada, Saowanee ; Apisitwanich, Somsak ; Nishida, Chizuko ; Matsuda, Yoichi. / Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata). In: Genetics and Molecular Biology. 2011 ; Vol. 34, No. 4. pp. 583-586.
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    abstract = "Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)",
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    author = "Kornsorn Srikulnath and Yoshinobu Uno and Kazumi Matsubara and Amara Thongpan and Saowanee Suputtitada and Somsak Apisitwanich and Chizuko Nishida and Yoichi Matsuda",
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    Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata). / Srikulnath, Kornsorn; Uno, Yoshinobu; Matsubara, Kazumi; Thongpan, Amara; Suputtitada, Saowanee; Apisitwanich, Somsak; Nishida, Chizuko; Matsuda, Yoichi.

    In: Genetics and Molecular Biology, Vol. 34, No. 4, 2011, p. 583-586.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata).

    AU - Srikulnath, Kornsorn

    AU - Uno, Yoshinobu

    AU - Matsubara, Kazumi

    AU - Thongpan, Amara

    AU - Suputtitada, Saowanee

    AU - Apisitwanich, Somsak

    AU - Nishida, Chizuko

    AU - Matsuda, Yoichi

    PY - 2011

    Y1 - 2011

    N2 - Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)

    AB - Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)

    KW - chromosomal mapping

    KW - FISH

    KW - interstitial telomeric site

    KW - Leiolepidinae

    KW - ribosomal RNA gene

    KW - telomeric sequence.

    U2 - 10.1590%2FS1415-47572011005000042

    DO - 10.1590%2FS1415-47572011005000042

    M3 - Article

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    EP - 586

    JO - Revista Brasileira de Genetica

    JF - Revista Brasileira de Genetica

    SN - 0100-8455

    IS - 4

    ER -