TY - JOUR
T1 - Comparing and combining distance-based and character-based approaches for barcoding turtles
AU - Reid, B
AU - Le, M
AU - McCord, William
AU - Iverson, John
AU - Georges, Arthur
AU - Bergmann, T
AU - Amato, George
AU - DeSalle, R
AU - Naro-Maciel, Eugenia
PY - 2011
Y1 - 2011
N2 - Molecular barcoding can serve as a powerful tool in wildlife forensics and may prove to be a vital aid in conserving
organisms that are threatened by illegal wildlife trade, such as turtles (Order Testudines). We produced cytochrome oxidase
subunit one (COI) sequences (650 bp) for 174 turtle species and combined these with publicly available sequences for 50
species to produce a data set representative of the breadth of the order. Variability within the barcode region was assessed,
and the utility of both distance-based and character-based methods for species identification was evaluated. For species in
which genetic material from more than one individual was available (n = 69), intraspecific divergences were 1.3% on average,
although divergences greater than the customary 2% barcode threshold occurred within 15 species. High intraspecific
divergences could indicate species with a high degree of internal genetic structure or possibly even cryptic species,
although introgression is also probable in some of these taxa. Divergences between species of the same genus were 6.4%
on average; however, 49 species were
AB - Molecular barcoding can serve as a powerful tool in wildlife forensics and may prove to be a vital aid in conserving
organisms that are threatened by illegal wildlife trade, such as turtles (Order Testudines). We produced cytochrome oxidase
subunit one (COI) sequences (650 bp) for 174 turtle species and combined these with publicly available sequences for 50
species to produce a data set representative of the breadth of the order. Variability within the barcode region was assessed,
and the utility of both distance-based and character-based methods for species identification was evaluated. For species in
which genetic material from more than one individual was available (n = 69), intraspecific divergences were 1.3% on average,
although divergences greater than the customary 2% barcode threshold occurred within 15 species. High intraspecific
divergences could indicate species with a high degree of internal genetic structure or possibly even cryptic species,
although introgression is also probable in some of these taxa. Divergences between species of the same genus were 6.4%
on average; however, 49 species were
KW - barcoding
KW - Characteristic Attribute Organization System
KW - species identification
KW - turtles
U2 - 10.1111/j.1755-0998.2011.03032.x
DO - 10.1111/j.1755-0998.2011.03032.x
M3 - Article
C2 - 21635698
VL - 11
SP - 956
EP - 967
JO - Molecular Ecology Notes
JF - Molecular Ecology Notes
SN - 1755-098X
ER -