Comparing and combining distance-based and character-based approaches for barcoding turtles

B Reid, M Le, William McCord, John Iverson, Arthur Georges, T Bergmann, George Amato, R DeSalle, Eugenia Naro-Maciel

    Research output: Contribution to journalArticle

    54 Citations (Scopus)

    Abstract

    Molecular barcoding can serve as a powerful tool in wildlife forensics and may prove to be a vital aid in conserving organisms that are threatened by illegal wildlife trade, such as turtles (Order Testudines). We produced cytochrome oxidase subunit one (COI) sequences (650 bp) for 174 turtle species and combined these with publicly available sequences for 50 species to produce a data set representative of the breadth of the order. Variability within the barcode region was assessed, and the utility of both distance-based and character-based methods for species identification was evaluated. For species in which genetic material from more than one individual was available (n = 69), intraspecific divergences were 1.3% on average, although divergences greater than the customary 2% barcode threshold occurred within 15 species. High intraspecific divergences could indicate species with a high degree of internal genetic structure or possibly even cryptic species, although introgression is also probable in some of these taxa. Divergences between species of the same genus were 6.4% on average; however, 49 species were
    Original languageEnglish
    Pages (from-to)956-967
    Number of pages12
    JournalMolecular Ecology Resources
    Volume11
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Turtles
    barcoding
    turtle
    turtles
    wildlife
    Testudines
    Genetic Structures
    DNA barcoding
    Cytochromes
    cytochromes
    introgression
    divergence
    Genes
    methodology
    genetic structure
    cytochrome
    forensic sciences
    species identification
    Datasets

    Cite this

    Reid, B ; Le, M ; McCord, William ; Iverson, John ; Georges, Arthur ; Bergmann, T ; Amato, George ; DeSalle, R ; Naro-Maciel, Eugenia. / Comparing and combining distance-based and character-based approaches for barcoding turtles. In: Molecular Ecology Resources. 2011 ; Vol. 11. pp. 956-967.
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    abstract = "Molecular barcoding can serve as a powerful tool in wildlife forensics and may prove to be a vital aid in conserving organisms that are threatened by illegal wildlife trade, such as turtles (Order Testudines). We produced cytochrome oxidase subunit one (COI) sequences (650 bp) for 174 turtle species and combined these with publicly available sequences for 50 species to produce a data set representative of the breadth of the order. Variability within the barcode region was assessed, and the utility of both distance-based and character-based methods for species identification was evaluated. For species in which genetic material from more than one individual was available (n = 69), intraspecific divergences were 1.3{\%} on average, although divergences greater than the customary 2{\%} barcode threshold occurred within 15 species. High intraspecific divergences could indicate species with a high degree of internal genetic structure or possibly even cryptic species, although introgression is also probable in some of these taxa. Divergences between species of the same genus were 6.4{\%} on average; however, 49 species were",
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    Reid, B, Le, M, McCord, W, Iverson, J, Georges, A, Bergmann, T, Amato, G, DeSalle, R & Naro-Maciel, E 2011, 'Comparing and combining distance-based and character-based approaches for barcoding turtles', Molecular Ecology Resources, vol. 11, pp. 956-967. https://doi.org/10.1111/j.1755-0998.2011.03032.x

    Comparing and combining distance-based and character-based approaches for barcoding turtles. / Reid, B; Le, M; McCord, William; Iverson, John; Georges, Arthur; Bergmann, T; Amato, George; DeSalle, R; Naro-Maciel, Eugenia.

    In: Molecular Ecology Resources, Vol. 11, 2011, p. 956-967.

    Research output: Contribution to journalArticle

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    AU - Reid, B

    AU - Le, M

    AU - McCord, William

    AU - Iverson, John

    AU - Georges, Arthur

    AU - Bergmann, T

    AU - Amato, George

    AU - DeSalle, R

    AU - Naro-Maciel, Eugenia

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    N2 - Molecular barcoding can serve as a powerful tool in wildlife forensics and may prove to be a vital aid in conserving organisms that are threatened by illegal wildlife trade, such as turtles (Order Testudines). We produced cytochrome oxidase subunit one (COI) sequences (650 bp) for 174 turtle species and combined these with publicly available sequences for 50 species to produce a data set representative of the breadth of the order. Variability within the barcode region was assessed, and the utility of both distance-based and character-based methods for species identification was evaluated. For species in which genetic material from more than one individual was available (n = 69), intraspecific divergences were 1.3% on average, although divergences greater than the customary 2% barcode threshold occurred within 15 species. High intraspecific divergences could indicate species with a high degree of internal genetic structure or possibly even cryptic species, although introgression is also probable in some of these taxa. Divergences between species of the same genus were 6.4% on average; however, 49 species were

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