Comparison of DNA extraction methods for identification of human remains

Cindy Lim, Mojca Keglovic, Dennis McNevin

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    After mass disasters, where the bodies of victims have been degraded, soft tissues are often completely lost, with the result that bones and teeth are all that remain for identification. The aim of this study was to investigate three DNA extraction methods that are currently used with degraded bones in forensic contexts: a silica-based extraction protocol used by the International Commission for Missing Persons (ICMP), a total demineralisation method used by the US Armed Forces DNA Identification Laboratory (AFDIL) and a standard organic DNA extraction method used by the Australian Federal Police (AFP). The methods were compared by real-time PCR quantitation and STR profiling. The silica and organic methods were not significantly different for either of these measures for bone but were both significantly more successful than the demineralisation method. Bovine serum albumin (BSA) was found to significantly improve the DNA yields from real-time PCR and the number of reportable alleles from STR typing, especially when using bone samples
    Original languageEnglish
    Pages (from-to)117-127
    Number of pages11
    JournalAustralian Journal of Forensic Sciences
    Volume44
    Issue number2
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    DNA
    Bone and Bones
    Silicon Dioxide
    Real-Time Polymerase Chain Reaction
    Disaster Victims
    Police
    Bovine Serum Albumin
    Body Remains
    Tooth
    Alleles

    Cite this

    Lim, Cindy ; Keglovic, Mojca ; McNevin, Dennis. / Comparison of DNA extraction methods for identification of human remains. In: Australian Journal of Forensic Sciences. 2012 ; Vol. 44, No. 2. pp. 117-127.
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    Comparison of DNA extraction methods for identification of human remains. / Lim, Cindy; Keglovic, Mojca; McNevin, Dennis.

    In: Australian Journal of Forensic Sciences, Vol. 44, No. 2, 2012, p. 117-127.

    Research output: Contribution to journalArticle

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