Concerning an Article by Ehl et al. False Premise Leads to False Conclusions

In their recent paper, entitled "With or without W? Molecular and cytogenetic markers are not sufficient for identification of environmentally-induced sex reversal in the bearded dragon", Ehl et al. [2021] demonstrate that a published PCR sex test developed for the bearded dragon (F4-F1 marker) [Quinn et al., 2010] is only partially diagnostic for sex because the sex-linked sequence upon which the test is based is subject to recombination with sex and therefore inappropriate for studies of sex reversal. The authors use the deficiencies of this now superseded PCR F1-F4 sex test developed by Quinn et al. [2010] to call into question the more recent and robust sex reversal work of Holleley et al. [2015] and subsequent work on this system in Pogona vitticeps [Castelli et al., 2021].