Constitutive expression of interferon-induced human MxA protein in transgenic tobacco plants does not confer resistance to a variety of RNA viruses

M. Frese, M. Prins, A. Ponten, R. W. Goldbach, O. Haller, P. Zeltz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

MxA is a key component in the interferon-induced antiviral defense in humans. After viral infections, MxA is rapidly induced and accumulates in the cytoplasm. The multiplication of many RNA viruses, including all bunyaviruses tested so far, is inhibited by MxA. These findings prompted us to express MxA in plants in an attempt to create resistance to tospoviruses. Here, we report the generation of transgenic tobacco plants that constitutively express MxA under the control of the 35 S cauliflower mosaic virus promotor. Northern and western blot analysis confirmed the expression of MxA in several transgenic plant lines. MxA expression had no obvious detrimental effects on plant growth and fertility. However, challenge experiments with tomato spotted wilt virus, tomato chlorotic spot virus, and groundnut ringspot virus revealed no increased resistance of MxA-transgenic tobacco plants to tospovirus infections. Neither was the multiplication of tobacco mosaic virus, cucumber mosaic virus and potato vires Y inhibited in MxA-transgenic plants. The results indicate that the expression of human MxA alone does not enhance virus resistance in planta.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalTransgenic Research
Volume9
Issue number6
DOIs
Publication statusPublished - 1 Dec 2000

Fingerprint

Tospovirus
Genetically Modified Plants
RNA Viruses
interferons
Interferons
Tobacco
tobacco
genetically modified organisms
Groundnut ringspot virus
Tomato chlorotic spot virus
transgenic plants
Viruses
Orthobunyavirus
plant fertility
Tomato spotted wilt virus
Cucumber mosaic virus
Tobacco mosaic virus
proteins
Cauliflower mosaic virus
Cucumovirus

Cite this

@article{90070231e225459ea5cd4ccb282c5e93,
title = "Constitutive expression of interferon-induced human MxA protein in transgenic tobacco plants does not confer resistance to a variety of RNA viruses",
abstract = "MxA is a key component in the interferon-induced antiviral defense in humans. After viral infections, MxA is rapidly induced and accumulates in the cytoplasm. The multiplication of many RNA viruses, including all bunyaviruses tested so far, is inhibited by MxA. These findings prompted us to express MxA in plants in an attempt to create resistance to tospoviruses. Here, we report the generation of transgenic tobacco plants that constitutively express MxA under the control of the 35 S cauliflower mosaic virus promotor. Northern and western blot analysis confirmed the expression of MxA in several transgenic plant lines. MxA expression had no obvious detrimental effects on plant growth and fertility. However, challenge experiments with tomato spotted wilt virus, tomato chlorotic spot virus, and groundnut ringspot virus revealed no increased resistance of MxA-transgenic tobacco plants to tospovirus infections. Neither was the multiplication of tobacco mosaic virus, cucumber mosaic virus and potato vires Y inhibited in MxA-transgenic plants. The results indicate that the expression of human MxA alone does not enhance virus resistance in planta.",
keywords = "Interferon, MxA protein, Nicotiana tabacum, Transgenic plants, Virus resistance",
author = "M. Frese and M. Prins and A. Ponten and Goldbach, {R. W.} and O. Haller and P. Zeltz",
year = "2000",
month = "12",
day = "1",
doi = "10.1023/A:1026526314875",
language = "English",
volume = "9",
pages = "429--438",
journal = "Transgenic Research",
issn = "0962-8819",
publisher = "Springer",
number = "6",

}

Constitutive expression of interferon-induced human MxA protein in transgenic tobacco plants does not confer resistance to a variety of RNA viruses. / Frese, M.; Prins, M.; Ponten, A.; Goldbach, R. W.; Haller, O.; Zeltz, P.

In: Transgenic Research, Vol. 9, No. 6, 01.12.2000, p. 429-438.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Constitutive expression of interferon-induced human MxA protein in transgenic tobacco plants does not confer resistance to a variety of RNA viruses

AU - Frese, M.

AU - Prins, M.

AU - Ponten, A.

AU - Goldbach, R. W.

AU - Haller, O.

AU - Zeltz, P.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - MxA is a key component in the interferon-induced antiviral defense in humans. After viral infections, MxA is rapidly induced and accumulates in the cytoplasm. The multiplication of many RNA viruses, including all bunyaviruses tested so far, is inhibited by MxA. These findings prompted us to express MxA in plants in an attempt to create resistance to tospoviruses. Here, we report the generation of transgenic tobacco plants that constitutively express MxA under the control of the 35 S cauliflower mosaic virus promotor. Northern and western blot analysis confirmed the expression of MxA in several transgenic plant lines. MxA expression had no obvious detrimental effects on plant growth and fertility. However, challenge experiments with tomato spotted wilt virus, tomato chlorotic spot virus, and groundnut ringspot virus revealed no increased resistance of MxA-transgenic tobacco plants to tospovirus infections. Neither was the multiplication of tobacco mosaic virus, cucumber mosaic virus and potato vires Y inhibited in MxA-transgenic plants. The results indicate that the expression of human MxA alone does not enhance virus resistance in planta.

AB - MxA is a key component in the interferon-induced antiviral defense in humans. After viral infections, MxA is rapidly induced and accumulates in the cytoplasm. The multiplication of many RNA viruses, including all bunyaviruses tested so far, is inhibited by MxA. These findings prompted us to express MxA in plants in an attempt to create resistance to tospoviruses. Here, we report the generation of transgenic tobacco plants that constitutively express MxA under the control of the 35 S cauliflower mosaic virus promotor. Northern and western blot analysis confirmed the expression of MxA in several transgenic plant lines. MxA expression had no obvious detrimental effects on plant growth and fertility. However, challenge experiments with tomato spotted wilt virus, tomato chlorotic spot virus, and groundnut ringspot virus revealed no increased resistance of MxA-transgenic tobacco plants to tospovirus infections. Neither was the multiplication of tobacco mosaic virus, cucumber mosaic virus and potato vires Y inhibited in MxA-transgenic plants. The results indicate that the expression of human MxA alone does not enhance virus resistance in planta.

KW - Interferon

KW - MxA protein

KW - Nicotiana tabacum

KW - Transgenic plants

KW - Virus resistance

UR - http://www.scopus.com/inward/record.url?scp=0034497319&partnerID=8YFLogxK

U2 - 10.1023/A:1026526314875

DO - 10.1023/A:1026526314875

M3 - Article

VL - 9

SP - 429

EP - 438

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 6

ER -