Construction of a genetic linkage map and QTL analysis in bambara groundnut

Nariman Ahmad; Endah Redjeki; Wai Ho; Siise Aliyu; Katie Mayes; Festo Massawe; Sean Mayes; F. Belzile

doi:10.1139/gen-2015-0153

Construction of a genetic linkage map and QTL analysis in bambara groundnut

Nariman Ahmad, Endah Redjeki, Wai Ho, Siise Aliyu, Katie Mayes, Festo Massawe, Sean Mayes, F. Belzile

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construction of the first genetic linkage map of bambara groundnut using a F3 population derived from a "narrow" cross between two domesticated landraces (Tiga Nicuru and DipC) with marked divergence in phenotypic traits. The map consists of 238 DArT array and SSR based markers in 21 linkage groups with a total genetic distance of 608.3 cM. In addition, phenotypic traits were evaluated for a quantitative trait loci (QTL) analysis over two generations. A total of 36 significant QTLs were detected for 19 traits. The phenotypic effect explained by a single QTL ranged from 11.6% to 49.9%. Two stable QTLs were mapped for internode length and growth habit. The identified QTLs could be useful for marker-assisted selection in bambara groundnut breeding programmes.

Original language	English
Pages (from-to)	459-472
Number of pages	14
Journal	Genome
Volume	59
Issue number	7
DOIs	https://doi.org/10.1139/gen-2015-0153
Publication status	Published - 2016
Externally published	Yes

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abstract = "Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construction of the first genetic linkage map of bambara groundnut using a F3 population derived from a {"}narrow{"} cross between two domesticated landraces (Tiga Nicuru and DipC) with marked divergence in phenotypic traits. The map consists of 238 DArT array and SSR based markers in 21 linkage groups with a total genetic distance of 608.3 cM. In addition, phenotypic traits were evaluated for a quantitative trait loci (QTL) analysis over two generations. A total of 36 significant QTLs were detected for 19 traits. The phenotypic effect explained by a single QTL ranged from 11.6% to 49.9%. Two stable QTLs were mapped for internode length and growth habit. The identified QTLs could be useful for marker-assisted selection in bambara groundnut breeding programmes.",

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author = "Nariman Ahmad and Endah Redjeki and Wai Ho and Siise Aliyu and Katie Mayes and Festo Massawe and Sean Mayes and F. Belzile",

year = "2016",

doi = "10.1139/gen-2015-0153",

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T1 - Construction of a genetic linkage map and QTL analysis in bambara groundnut

AU - Ahmad, Nariman

AU - Redjeki, Endah

AU - Ho, Wai

AU - Aliyu, Siise

AU - Mayes, Katie

AU - Massawe, Festo

AU - Mayes, Sean

AU - Belzile, F.

PY - 2016

Y1 - 2016

N2 - Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construction of the first genetic linkage map of bambara groundnut using a F3 population derived from a "narrow" cross between two domesticated landraces (Tiga Nicuru and DipC) with marked divergence in phenotypic traits. The map consists of 238 DArT array and SSR based markers in 21 linkage groups with a total genetic distance of 608.3 cM. In addition, phenotypic traits were evaluated for a quantitative trait loci (QTL) analysis over two generations. A total of 36 significant QTLs were detected for 19 traits. The phenotypic effect explained by a single QTL ranged from 11.6% to 49.9%. Two stable QTLs were mapped for internode length and growth habit. The identified QTLs could be useful for marker-assisted selection in bambara groundnut breeding programmes.

AB - Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construction of the first genetic linkage map of bambara groundnut using a F3 population derived from a "narrow" cross between two domesticated landraces (Tiga Nicuru and DipC) with marked divergence in phenotypic traits. The map consists of 238 DArT array and SSR based markers in 21 linkage groups with a total genetic distance of 608.3 cM. In addition, phenotypic traits were evaluated for a quantitative trait loci (QTL) analysis over two generations. A total of 36 significant QTLs were detected for 19 traits. The phenotypic effect explained by a single QTL ranged from 11.6% to 49.9%. Two stable QTLs were mapped for internode length and growth habit. The identified QTLs could be useful for marker-assisted selection in bambara groundnut breeding programmes.

KW - Bambara groundnut

KW - Breeding

KW - Genetic mapping

KW - QTL analysis

KW - genetic mapping

KW - bambara groundnut

KW - breeding

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U2 - 10.1139/gen-2015-0153

DO - 10.1139/gen-2015-0153

M3 - Article

SN - 0831-2796

VL - 59

SP - 459

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JO - Genome

JF - Genome

IS - 7

ER -

Construction of a genetic linkage map and QTL analysis in bambara groundnut

Abstract

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