Control of Penicillium roqueforti (Thom) infection in cultures of Drosophila melanogaster (Meigen) (Diptera: Drosophilidae)

Clare Holleley, Ailsa Hocking, Tracey Schubert, Michael Whitehead

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Microbial contamination of artificial insect food media can jeopardise the viability, productivity and survival of many insect cultures, including Drosophila melanogaster. Here we investigated and improved upon control methods for one common contaminant, Penicillium roqueforti. We found that the combined effect of methyl p-hydroxybenzoate (23.7 mM), propionic acid (67.5 mM) and sorbic acid (8.9 mM) (PSNPS treatment) was the most effective of the four candidate treatments, at inhibiting the growth of P. roqueforti. PSNPS treatment inhibited 100% of visible P. roqueforti growth for 21 days (a complete D. melanogaster life cycle) and thus reduced the risk of transmitting infection to the next generation. Although the PSNPS treatment negatively affected the two D. melanogaster fitness components, survivorship (number of adults) and biomass (live weight), it did not prevent successful reproduction and is suitable for short-term treatment of P. roqueforti infections.
Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalAustral Entomology
Volume47
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Drosophilidae
Penicillium
Drosophila melanogaster
insect
acid
survivorship
infection
sorbic acid
insects
viability
fitness
life cycle
microbial contamination
propionic acid
productivity
food
pollutant
control methods
life cycle (organisms)
biomass

Cite this

Holleley, Clare ; Hocking, Ailsa ; Schubert, Tracey ; Whitehead, Michael. / Control of Penicillium roqueforti (Thom) infection in cultures of Drosophila melanogaster (Meigen) (Diptera: Drosophilidae). In: Austral Entomology. 2008 ; Vol. 47. pp. 149-152.
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abstract = "Microbial contamination of artificial insect food media can jeopardise the viability, productivity and survival of many insect cultures, including Drosophila melanogaster. Here we investigated and improved upon control methods for one common contaminant, Penicillium roqueforti. We found that the combined effect of methyl p-hydroxybenzoate (23.7 mM), propionic acid (67.5 mM) and sorbic acid (8.9 mM) (PSNPS treatment) was the most effective of the four candidate treatments, at inhibiting the growth of P. roqueforti. PSNPS treatment inhibited 100{\%} of visible P. roqueforti growth for 21 days (a complete D. melanogaster life cycle) and thus reduced the risk of transmitting infection to the next generation. Although the PSNPS treatment negatively affected the two D. melanogaster fitness components, survivorship (number of adults) and biomass (live weight), it did not prevent successful reproduction and is suitable for short-term treatment of P. roqueforti infections.",
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Control of Penicillium roqueforti (Thom) infection in cultures of Drosophila melanogaster (Meigen) (Diptera: Drosophilidae). / Holleley, Clare; Hocking, Ailsa; Schubert, Tracey; Whitehead, Michael.

In: Austral Entomology, Vol. 47, 2008, p. 149-152.

Research output: Contribution to journalArticle

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AU - Whitehead, Michael

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