Cryopreservation and other assisted reproductive technologies for the conservation of threatened amphibians and reptiles: Bringing the ARTs up to speed

John Clulow, Simon Clulow

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Amphibians and reptiles are experiencing serious declines, with the number of threatened species and extinctions growing rapidly as the modern biodiversity crisis unfolds. For amphibians, the panzootic of chytridiomycosis is a major driver. For reptiles, habitat loss and harvesting from the wild are key threats. Cryopreservation and other assisted reproductive technologies (ARTs) could play a role in slowing the loss of amphibian and reptile biodiversity and managing threatened populations through genome storage and the production of live animals from stored material. These vertebrate classes are at different stages of development in cryopreservation and other ARTs, and each class faces different technical challenges arising from the separate evolutionary end-points of their reproductive biology. For amphibians, the generation of live offspring from cryopreserved spermatozoa has been achieved, but the cryopreservation of oocytes and embryos remains elusive. With reptiles, spermatozoa have been cryopreserved in a few species, but no offspring from cryopreserved spermatozoa have been reported, and the generation of live young from AI has only occurred in a small number of species. Cryopreservation and ARTs are more developed and advanced for amphibians than reptiles. Future work on both groups needs to concentrate on achieving proof of concept examples that demonstrate the use of genome storage and ARTs in successfully recovering threatened species to increase awareness and support for this approach to conservation.

Original languageEnglish
Pages (from-to)1116-1132
Number of pages17
JournalReproduction, Fertility and Development
Volume28
Issue number8
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint Dive into the research topics of 'Cryopreservation and other assisted reproductive technologies for the conservation of threatened amphibians and reptiles: Bringing the ARTs up to speed'. Together they form a unique fingerprint.

Cite this